...Results of Gel Electrophoresis For the gel electrophoresis of the genomic DNA, a band of genomic DNA (roughly 23kbp) is observed near the top of the gel. Further down the gel is another block of bands (ranging from 100bp ~ 2000bp) which is the degraded RNA. My group’s sample (lanes 16&17) shows similar band patterns to that of other groups where genomic DNA and RNA bands are seen but in differing quantities of DNA since some bands appear more faint than the others. In the gel electrophoresis of the plasmid DNA, 4 distinct bands of DNA are observed. The top band of roughly 23kbp is the nicked/relaxed circular plasmid. Below it is another band of roughly 9500bp of linear plasmid DNA. The following band of around...
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...Taster Genomic Analysis Lab Report Laboratory Goals: 1. Determine Taster Phenotype 2. Isolate DNA from each individual 3. Determine Taster Genotype Hypothesis: If I am a taster, then my genotype for PTC taster must be either TT (homozygous dominant) or Tt (heterozygous) I – Results: This experiment aimed to investigate the allele frequency of the PTC taster gene (TAS2R38) in a small population, represented by the students in class. The genotype obtained from genomic analysis (via PCR and gel electrophoresis) confirmed that the genotypic result is consistent with the phenotypic result observed at the beginning of the lab. However, DNA fragments of 3 lab subjects didn’t show up on the gel. The allele frequencies can’t be calculated because the data is insufficient to apply the Hardy-Weinberg equation. There are many factors that might be contributed to the invisibility of these DNA fragments, most likely accidental errors. For example, the DNA wasn’t loaded onto the gel probably, or the DNA for some reason didn’t sink to the bottom of the well, or just simply there was not enough DNA. To determine the genotypic profile of the students PTC gene, DNA samplers from each individual was collected from saliva. Using premade PTC primers (short oligonucleotides), a DNA template that encoded the PTC gene (approximately, 303 bp) was amplified by PCR. After amplification, the produced DNA fragments were digested with Fnu4H1 to identify if the lab subjects have a C...
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...DNA Fingerprinting Using Agarose Gel S. Aaron Sowards Bio 122 Lab 04 Brianna Adanitsch Jakob Lester Minhenga Ngijoi 2/21/18 Dr. Chad R. Sethman Abstract DNA fingerprinting is the process of analyzing an individual’s DNA base-pair patterns. The DNA fingerprinting lab involved identifying the suspect using Agarose Gel and Polymerase Chain Reaction. It was found that suspect two s DNA matched the crime scene DNA. This is known because suspect twos DNA traveled the same distance as the crime scene DNA. DNA Fingerprinting Using Agarose Gel Introduction In 1984 Dr. Alex Jeffreys came up with deoxyribonucleic acid (DNA) fingerprinting, which is also known as DNA profiling or DNA typing. DNA fingerprinting is the analyzing...
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...Introduction The profound importance for microorganisms to operate at a maximum efficiency has lead to adaptations allowing for groups of processes to be functional when resources are available, while on the contrary remaining “dormant” when not in need. This has been accomplished at the molecular level by configuring clusters of genes together on the genome into operons that elicit a processive response in the presence of a specific metabolite. The Lac operon is responsible for the cleaving of the disaccharide lactose into two products. A myriad of components control the expression of the Lac operon when two conditions are met. First, the substrate, lactose, must be present. Second, no better substrate for example, glucose, is present (2). The three structural genes in the Lac operon are lacZ, lacY, and lacA. The gene lacZ encodes the tetramer, ß-galactosidase, which is responsible for hydrolyzing the ß-1,4 glycosidic linkage between galactose and glucose in lactose. The transport of lactose into the cell via the enzyme lactose permease is encoded by the gene lacY. The lacA gene encodes the enzyme, galactoside transacetylase, a trimer that transfers an acetyl group from acetyl-CoA to galactosides. Activation of these genes is dependent on the activity of a promoter and three operators based on the nutritional and environmental conditions available to the cell. The lac operon is a negatively controlled inducible operon that utilizes the product of the regulator gene lacI, to...
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...differentiation of cardiac tissue, and the study of cytokines in the differentiation of cardiac tissue. I hope that one day it will be possible to grow whole hearts from a patients' own cells to replace their damaged or diseased hearts. This could effectively eliminate organ transplant waitlists for hearts. My interest in biomedical engineering over other science related fields stems from the fact that my mother is a nurse and my father was a lab technician while he was in the military. Sometimes he would take me to his work and I could see how the lab was set up. He would let me look through the microscopes and sometimes explain what he was doing. He would often tell me that he was surprised that I actually understood most of what he told me. Before attending UT, I earned a biotechnology certificate at Temple College. During the program I gained hands-on experience in many...
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...Rebekah Wells HBS 1st Period Case Report 1.2.3 Bone Detectives Introduction: While a couple was out for their morning run, they happened to stumble upon what looked like a skull. They called the police and the police discovered two skeletons lying side by side. The bones included the skull, pelvis, humorous, and tibia. With these, the forensic anthropologists have determined the person to be a healthy white woman from 5 feet 6 inches to 6 feet tall using measurements and calculations of the bones. Summary of Findings: The bones found were labeled as female for many reasons. A lot of the evidence we acquired pointed in this direction. The pelvis was a huge indicator because the sub-pubic angle measured 95⁰, and in the identification...
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...Introduction: Vitamin D is critical for embryonic skeletal formation and long bone growth. Without vitamin D, the hypertrophic cell zone will expand because the cartilage fails to calcify leading to rickets. Protein disulfide isomerase A3 (PDIA3), also known as ERp60, ERp57, Grp58, and 1,25-MARRS, is a multifunctional protein that has been associated with rapid membrane-initiated signaling by 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3) in several cell types. Knockdown of PDIA3 in osteoblast-like cell line MC3T3 showed that reduction of PDIA3 expression led to remarkable decrease of PKC activation with 1,25 (OH) 2VitD3 treatment as well as a decrease in expression of several osteogenic genes compared to wild type cells, which indicated the important role of PDIA3 in vitamin D-regulated cartilage and bone development. However, the mechanism involved still remains unclear. The fact motivate us to generate the PDIA3 knockout mice which will serve as a good model to further explore the physiological function of PDIA3 and to understand the mechanism involved in PDIA3-mediated 1,25VitD3 signal pathway. To date, the major problems we are facing during the generation an ERp60 Knockout mice is the lack of a liable genotyping method to distinguish the Knockout mice from the heterozygous mice. No knockout mice available from the previous breeding along with very lower percentage of wild type mice which did not match to the Mendel’s rule of Segregation. Therefore, the object...
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...Eyeless mutation gene located within the second intron of Drosophila melanogaster Justin Lazarus Genetic 300 Abstract The following experiment was conduct over a several week time span to determine and identify the mutation that is causing the eyeless mutation within the Drosophila melanogaster fruit flies. The experiment included genome sequencing and comparison between the Drosophila melanogaster wild type and the Drosophila melanogaster eyeless type. After combining the two different phenotypes. We determined that we were unable to visualize the mutation at a chromosomal level, as both wild-type and eyeless flies looked similar. The experiment involved electrophoresis and Polymerase Chain Reaction (PCR) through which we were able to isolate and amplify the needed DNA eyeless DNA. The difference between the wild-type Drosophila melanogaster and the eyeless Drosophila melanogaster is approximately only 500-nucleotide base pairs. As we see the eyeless phenotype is approximately 3000 base pairs in length while the wild-type phenotype is approximately 2500 nucleotides base pairs in length, a difference of about 500 base pairs. After completing nucleotide sequencing and comparing our data on the blast website, we determined that the eyeless mutation has being interest exons two and three, but more specifically the mutation itself was located within the second intron at base pairs 8264 to 9212. Introduction In the early 20th century scientists had already been acquainted with...
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...BTEC Level 3 Extended Diploma in Science Student Name: | | | | | | | | Date Submitted: | | | | Unit: | 18 – | | | | | | | I certify that the attached work is original and my own:…………………………………………… Student Signature | | | Assessment Title: | Genetics & Genetic Engineering | | | | | | | | | Lecturer: | | | | | | | | | IV Name | M. Silverwood | | | | | | | | IV date: | 3/2/13 | Assessment Criteria: | Task (criteria) Completed:- P1 | P2 | P3 | P4 | P5 | P6 | P7 | M1 | M2 | | M3 | | M4 | | D1 | D2 | | | | D3 | | | Guidance for this Assignment : | Date set: Task 1 27/1/14 | Date set: Task 2 10/2/14 | Date set: Task 3 24/2/14 | Date set: Task 4 10/3/14 | Remember! | * Your attention is drawn to the comments made in the Course Handbook with regard to both the completion and submission of work * Assessment deadlines must be met * Any student found guilty of cheating or plagiarism may be withdrawn from the unit at the discretion of the Assessment Board. | Internal verification: | Date: | | Name | | | | Signature | | Aim and purpose:-To develop understanding of the principles of Mendelian genetics and to develop knowledge and practical techniques used in commercial, analytical and research laboratories | | GRADING CRITERIA To achieve a pass grade the evidence must show that the learner is able to: | To achieve...
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...DNA profiling (also called DNA testing, DNA typing, or genetic fingerprinting) is a technique employed by forensic scientists to assist in the identification of individuals by their respective DNA profiles. DNA profiles are encrypted sets of numbers that reflect a person's DNA makeup, which can also be used as the person's identifier. DNA profiling should not be confused with full genome sequencing.[1] It is used in, for example, parental testing and criminal investigation. Although 99.9% of human DNA sequences are the same in every person, enough of the DNA is different to distinguish one individual from another, unless they are monozygotic twins.[2] DNA profiling uses repetitive ("repeat") sequences that are highly variable,[2] called variable number tandem repeats (VNTR), particularly short tandem repeats (STR)s. VNTRs loci are very similar between closely related humans, but so variable that unrelated individuals are extremely unlikely to have the same VNTRs. The DNA profiling technique was first reported in 1984[3] by Sir Alec Jeffreys at the University of Leicester in England,[4] and is now the basis of several national DNA databases. Dr. Jeffreys's genetic fingerprinting was made commercially available in 1987, when a chemical company, Imperial Chemical Industries (ICI), started a blood-testing centre in England.[5] Contents [hide] 1 DNA profiling process 1.1 RFLP analysis 1.2 PCR analysis 1.3 STR analysis 1.4 AmpFLP ...
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...experiments in this paper done through instruction in graduate course: Biotechnology laboratory in the natural sciences and mathematics department at the university of texas at dallas, RICHARDSON, TX 75080 march 2016 [Company name] | [Company address] ------------------------------------------------- γ-globin analysis by expression profiling through RT-qPCR, quantification through ELISA, and oxidative stress management analysis by FACS from KU812F cells under treatment by δ aminolevulinic acid, succinylacetone, and N-methyl mesoporphyrin Shaan Sarode, Jose Cordero, and Dr. Li Liu experiments in this paper done through instruction in graduate course: Biotechnology laboratory in the natural sciences and mathematics department at the university of texas at dallas, RICHARDSON, TX 75080 march 2016 [Company name] | [Company address] ------------------------------------------------- γ-globin analysis by expression profiling through RT-qPCR, quantification through ELISA, and oxidative stress management analysis by FACS from KU812F cells under treatment by δ aminolevulinic acid, succinylacetone, and N-methyl mesoporphyrin Shaan Sarode, Jose Cordero, and Dr. Li Liu ABSTRACT Hemoglobinopathies refer to a group of blood related disorders that encompass important disease such as thalassemia and sickle cell disease. Because many of these disease are hereditary more aggressive genetic therapies are showing promise as possible avenues of treatment. One such method is to re-express...
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...Name: Sabina Shrestha Name partner: Marieke RoosStudent Numbers: 1215671/4228073 Practicum assistants: Brijith Thomas,Room: 1 Joanna Pawlak, Lara van der Woude, Date: 12/12/2014 Valerie SelsEmail: saburo_aikini441@yahoo.com | Lab Report DNA: Plasmids and Nucleases 1. Abstract The goal of this practicum was to isolate plasmid DNA from Escherichia coli (E. coli), to identify it, to prove that the plasmid is circular and double-stranded and to give bacterial cells new genetic properties via transformation. An unknown plasmid S was isolated from the bacterial stain Escherichia coli (E. coli). Then its purity was determined by calculating the ratio A260/A280. After that, the unknown plasmid S was identified, followed by the determination of its antibiotic resistance gene. Subsequently, a new culture of E. coli DH5α was transformed by using the isolated plasmids where only the transformed cells survived. Finally, the plasmid-DNA was treated with exonuclease V to prove the circular and double-stranded property of plasmid. The isolated plasmid S was identified to be pCTB2 which was found to be have two forms: (I) relaxed & (II) supercoiled. The ratio A260/A280 gave the value of 1.65 indicating the presence of proteins (RNA/contaminated DNA). Furthermore, the plasmids were partially digested...
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...Chemistry Modern Analytical Chemistry David Harvey DePauw University Boston Burr Ridge, IL Dubuque, IA Madison, WI New York San Francisco St. Louis Bangkok Bogotá Caracas Lisbon London Madrid Mexico City Milan New Delhi Seoul Singapore Sydney Taipei Toronto McGraw-Hill Higher Education A Division of The McGraw-Hill Companies MODERN ANALYTICAL CHEMISTRY Copyright © 2000 by The McGraw-Hill Companies, Inc. All rights reserved. Printed in the United States of America. Except as permitted under the United States Copyright Act of 1976, no part of this publication may be reproduced or distributed in any form or by any means, or stored in a data base or retrieval system, without the prior written permission of the publisher. This book is printed on acid-free paper. 1 2 3 4 5 6 7 8 9 0 KGP/KGP 0 9 8 7 6 5 4 3 2 1 0 ISBN 0–07–237547–7 Vice president and editorial director: Kevin T. Kane Publisher: James M. Smith Sponsoring editor: Kent A. Peterson Editorial assistant: Jennifer L. Bensink Developmental editor: Shirley R. Oberbroeckling Senior marketing manager: Martin J. Lange Senior project manager: Jayne Klein Production supervisor: Laura Fuller Coordinator of freelance design: Michelle D. Whitaker Senior photo research coordinator: Lori Hancock Senior supplement coordinator: Audrey A. Reiter Compositor: Shepherd, Inc. Typeface: 10/12 Minion Printer: Quebecor Printing Book Group/Kingsport Freelance cover/interior designer: Elise Lansdon Cover image: © George Diebold/The...
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...Unit II: Genetics Brief Overview Reading: Chapters 3, 4, 9-12, 14 (Note: you have reviewed much of this already) The earth is teeming with living things. We can easily see some of the larger organisms—trees, grass, flowers, weeds, cats, fish, squirrels, dogs, insects, spiders, snails, mushrooms, lichens. Other organisms are everywhere, in the air, in water, soil and on our skin, but are too small to see with the naked eye—bacteria, viruses, protists (single celled eukaryotes such as amoebae), and tiny plants and animals. Life is remarkable in its complexity and diversity, and yet it all boils down to a very simple idea—the instructions for making all this life are written in nucleic acids, usually DNA. Most organisms have a set of DNA that contains the instructions for making that creature. This DNA contains four “letters” in which these instructions are written—A, T, G, and C. The only difference between the code for a dog and the code for a geranium is in the order of those letters in the code. If you took the DNA from a human and rearranged the letters in the right way, you could produce an oak tree—arrange them slightly differently and you would have a bumble bee—arrange them again and you would have the instructions for making a bacterium. Acting through more than two billion years, the process of evolution has taken one basic idea—a molecular code that uses four letters—and used it over and over, in millions of combinations to produce a dazzling array of life forms...
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...Clostridium difficile, also known as "CDF/cdf", or "C. diff", is a species of Gram-positive bacteria of the genus Clostridium that causes severe diarrhea and other intestinal disease when competing bacteria in the gut flora have been wiped out by antibiotics. Clostridia are anaerobic, spore-forming rods. C. difficile is the most serious cause of antibiotic-associated diarrhea (AAD) and can lead to pseudomembranous colitis, a severe inflammation of the colon, often resulting from eradication of the normal gut flora by antibiotics. In a very small percentage of the adult population, C. difficile bacteria naturally reside in the gut. Other people accidentally ingest spores of the bacteria while they are patients in a hospital, nursing home, or similar facility. When the bacteria are in a colon in which the normal gut flora has been destroyed (usually after a broad-spectrum antibiotic such as clindamycin has been used), the gut becomes overrun with C. difficile. This overpopulation is harmful because the bacteria release toxins that can cause bloating and diarrhea, with abdominal pain, which may become severe. C. difficile infections are the most common cause of pseudomembranous colitis, and in rare cases this can progress to toxic megacolon, which can be life-threatening. Latent symptoms of C. difficile infection often mimic some flu-like symptoms and can mimic disease flare in patients with inflammatory bowel disease-associated colitis.[4] Mild cases of C. difficile...
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