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Glomerular Diffusion Lab Report

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AT THE CELLULAR LEVEL:-
Glucose is a hydrophilic molecule and, as such, cannot diffuse across cell membranes. The two families of glucose transporters are the sodium-glucose contransporters (SGLTs) and the facilitated-diffusion GLUT transporters. SGLTs are localized in the apical membranes of simple epithelia (intestine and proximal tubules of the kidney) and are involved in the transepithelial transport of glucose. GLUTs provide for sodium-independent transmembrane transport of glucose by facilitated diffusion. GLUT1 and GLUT3 are widely expressed and are high-affinity, low-capacity transporters. These GLUT isoforms are linked to high-affinity hexokinases. Hexokinases phosphorylate glucose to form glucose-6-phosphate (G6P). Because G6P does not bind to GLUTs, G^P cannot leave the cell. Consequently, the hexokinase reaction commits glucose to metabolic pathway. GLUT2 is a low-affinity, high-capacity isoform expressed in the liver, pancreatic islet beta cell, and basolateral side of intestinal and renal tubule cells. In the liver and beta cells, GLUT2 …show more content…
Acetyl CoA is then further metabolized in the TCA cycle and the closely coupled process of oxidative phosphorylation via the electron transport chain. This second stage of oxidation yields almost 20 times more ATP than glycolysis does. Thus, the TCA cycle and oxidative phosphorylation are very efficient means of generating ATP from glucose. However, molecular O2 is required. This is the reason that humans need to breathe air, and oxidative phosphorylation can proceed only as fast as the respiratory and cardiovascular systems can deliver O2 to tissues. Therefore, even tissues with mitochondria rely on anaerobic glycolysis for some needs. The process of oxidative phosphorylation is also a major contributor to the generation of reactive oxygen species (ROS), which impose oxidative stress that is harmful to

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