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How Enzymes Function

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HOW ENZYMES FUNCTION

SHANITA HAMLIN
BIOL1402L-165
DR. MERCEDES MONDECAR
09/27/2012

INTRODUCTION:
Enzymes are proteins produced by living cells, which regulate chemical reactions in the body.
Enzyme activity can be affected by

The experiments conducted were done to show that under optimum conditions; such as the ideal temperature, the right pH, and a higher enzyme or substrate concentration, the activity of the catalase enzyme would increase.
HYPOTHESIS:
Exercise 5.1 Catalase Activity-Test tube one, which contains catalase buffered at pH 7 and hydrogen peroxide, will produce the highest bubble height because it contains the proper substrate/enzyme combo.
Exercise 5.2 Effect of temperature on Enzyme Activity-The test tube that is place in the incubator will produce the highest bubble height, because warm temperatures speed chemical reactions.
Exercise 5.3 Effect of Concentration on Enzyme Activity-Test tube three will produce the greatest bubble height, because it has highest enzyme concentration out of all of the tubes.
Exercise 5.4 Effect of pH on Enzyme Activity-The test tube with a pH 7, will produce the highest bubble column, since pH 7 is the optimum pH for catalase.

EQUIPMENT:
Exercise 5.1 Catalase Activity- Materials used were three clean test tubes, a black sharpie, and a ruler. Additional materials included 10mm of catalase buffered at pH 7.0, and 40mm of hydrogen peroxide for test tube. Ten mm of water, and 40mm of hydrogen peroxide were used for test tube 2. And, 10 mm of catalase, and 40mm of sucrose solution were used for test tube 3.
Exercise 5.2 Effect of Temperature- Materials used included three clean test tubes, a black sharpie, 10mm catalase buffered at pH 7.0, refrigerator, incubator, warm water bath, and 40 mm hydrogen peroxide, and a ruler.
Exercise 5.3 Effect of Concentration on Enzyme Activity-

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