Premium Essay

Invertase Lab Report

Submitted By
Words 513
Pages 3
During this experiment our group was tasked to answer the question of how many of drops of pH (either HCl or NaOH) it would take to render the enzyme useless and stop the digestion and breaking down of the substrate. The enzyme that we were using to answer this question was invertase. Invertase is an enzyme that splits the substrate sucrose into the component parts glucose and fructose, while sucrose, our substrate, is common table sugar. Throughout this experiment we learned a lot more about the resistance some enzymes have and how they break down their substrates. We conducted this experiment in a very basic and repetitive way. We started off by eight test tubes with all of them with filled with a dropper of invertase and sucrose. Half of the test tubes were filled with HCl and the other half NaOH. They were filled with 5, 10, 15, and 25 drops of each pH. We then put ten drops of Benedict’s Solution into each of the test tubes and boiled them either until …show more content…
We also can argue that invertase is a very resistant enzyme and it can still digest and break down a substrate when put into unknown conditions, considering many enzymes require special conditions to work properly. We came up with these arguments because in every test the enzyme and substrate reacted positively and turned orange. Throughout this experiment we learned many things about the enzyme Invertase. One thing we learned was that some enzymes don’t necessarily need extremely specific conditions to still react with its substrate. From this we can also conclude that the substrate sucrose is also very resistant as it is also reacting with the enzyme and still binding to the enzyme’s active

Similar Documents

Free Essay

Lab Report Instructions

...London School of Engineering and Materials Science Laboratory report writing instructions DEN101 - Fluid Mechanics 1 Flow Rate Measurement Experiment A. Student Student Number: 1234567 Version 2.0, 27 November 2010 Template for Word 97-2003 Abstract This document explains what is expected in your Fluids 1 lab report. The sections that should be covered are outlined and a structure you could follow is proposed. Detailed advice on how to edit the report is given. The document concludes with the marking criteria for this lab report. Table of Contents Abstract 2 1. Introduction 3 1.1. Writing 3 1.2. Editing and formatting 3 1.3. Content of the introduction 4 2. Background and theory 4 3. Apparatus 4 4. Test 4 5. Experimental procedure 4 6. Results 5 7. Discussion 5 8. Conclusions 5 9. References 5 10. Appendix A: Marking criteria 6 Introduction Before starting to write a report, you should think about what is your audience. Am I writing for colleagues who want a lot of detail how it is done, or am I writing for my boss who just wants an executive summary as he has no time for details? In general, there is not a single type of audience and we have to make our writing suitable for the detailed read, as well as the fast perusal. To understand what is required from you in this report, please have a look at the marking criteria in the Appendix. 1 Writing To limit...

Words: 2017 - Pages: 9

Free Essay

Invertase/ Phosphatase Results

...Lindsey M. Maurice-Walker Week 6/7 Results Biochemistry Lab March 14, 2013 Results: Table 1 and Table 2, given below, display the average absorbance of the samples, the amount of protein in each sample, the activity value, the specific activity, and the molar absorptivity calculated from the above equations for Phosphatase and Invertase. Table 1. Value Summary for Phosphatase | | Abs400 | mg of protein | Units (activity)(mg/min) | Specific Activity(ug/min/mg) | CYE | 0.296333333 | 0.003753 | 3.7079E-07 | 0.000099 | F1 | 0.832 | 0.000732 | 1.23686E-06 | 0.001689704 | F2 | 0.365333333 | 0.002678 | 4.8235E-07 | 0.000180116 | F3 | 0.152666667 | 0.0003375 | 1.38507E-07 | 0.000410 | Negative | 0.067 | | | | Positive | 1.304 | | | | Molar Abs. (M-1 cm-1) | 20616.67 | | | | The values located in the column labeled, “Abs400,” were obtained by averaging the four absorbance readings of the certified yeast extract (CYE), the controls, and samples F1-F3 at 400nm. The other values were found using the equations listed in the Methods section of this report. Table 2. Value Summary for Invertase | | Abs540 | mg of protein | Units (activity)(mmol/min) | Specific Activity (umol/min/mg) | CYE | 1.33225 | 0.003753 | 3.36E-06 | 0.000894706 | F1 | 2.782 | 0.000732 | 7.29E-06 | 0.009953591 | F2 | 2.84875 | 0.002678 | 7.47E-06 | 0.002788235 | F3 | 2.874 | 0.0003375 | 7.54E-06 | 0.022326842 | Negative | 0.093 | | | | Positive | 3.0455 | | | ...

Words: 293 - Pages: 2

Free Essay

Chem 222

...DETERMINATION OF CONCENTRATION OF MONOSACCHARIDES BY POLARIMETRY Background Polarimetry is is a very useful method to analyze chiral substances. The magnitude and direction of rotation of the plane of lineraly polarized light by a chiral compound is a specific physical property of the compound that can be used to characterize it. Most biomolecules are chiral and hence rotate polarized light. In this experiment you will study the optical rotation of a series of mixed monosaccharide solutions and determine the relative concentrations of them. The instrument used to study optical rotation in chiral molecules is called a polarimeter. A polarimeter takes light vibrating in all planes, isolates the light vibrating in a single plane, projects the light through a tube filled with a solution of chiral compound, and measures the amount of rotation. The observed angle of rotation of the plane of polarization by an optically active liquid, solution, or (more rarely) gas or solid is usually denoted by the symbol α. The angle may be either positive (+) or negative (-) depending on whether the rotation is clockwise, that is, to the right (dextro) or counterclockwise, that is, to the left (levo) as seen by an observer towards whom the beam of polarized light travels (this is opposite from the direction of rotation viewed along the light beam). Biot discovered that the observed rotation is proportional to the length l of the cell or tube containing the optically active liquid or solution...

Words: 1450 - Pages: 6

Free Essay

Registered General Nursing Student

...Section A: Basic Microbiology 1 SCOPE AND HISTORICAL DEVELOPMENTS IN MICROBIOLOGY “Science contributes to our culture in many ways, as a creative intellectual activity in its own right, as a light which has served to illuminate man’s place in the uni-verse, and as the source of understanding of man’s own nature” —John F. Kennedy (1917–63) The President of America The bacterium Escherichia coli INTRODUCTION AND SCOPE MICROBIOLOGY is a specialized area of biology (Gr. bios-life+ logos-to study) that concerns with the study of microbes ordinarily too small to be seen without magnification. Microorganisms are microscopic (Gr. mikros-small+ scopein-to see) and independently living cells that, like humans, live in communities. Microorganisms include a large and diverse group of microscopic organisms that exist as single cell or cell clusters (e.g., bacteria, archaea, fungi, algae, protozoa and helminths) and the viruses, which are microscopic but not cellular. While bacteria and archaea are classed as prokaryotes (Gr. pro-before+ karyon-nucleus) the fungi, algae, protozoa and helminths are eukaryotes (Gr. eu-true or good+ karyon-nucleus). Microorganisms are present everywhere on earth, which includes humans, animals, plants and other living creatures, soil,water and atmosphere. Microorganisms are relevant to all of our lives in a multitude of ways. Sometimes, the influence of microorganisms on human life is beneficial, whereas at other times, it is detrimental. For example...

Words: 9515 - Pages: 39