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Jatropha Gossypiifolia L. (Euphorbiaceae): a Review of Traditional Uses, Phytochemistry, Pharmacology, and Toxicology of This Medicinal Plant

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Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2014, Article ID 369204, 32 pages http://dx.doi.org/10.1155/2014/369204

Review Article Jatropha gossypiifolia L. (Euphorbiaceae): A Review of Traditional Uses, Phytochemistry, Pharmacology, and Toxicology of This Medicinal Plant
Juliana Félix-Silva,1 Raquel Brandt Giordani,2 Arnóbio Antonio da Silva-Jr,1 Silvana Maria Zucolotto,2 and Matheus de Freitas Fernandes-Pedrosa1
1

Laborat´rio de Tecnologia & Biotecnologia Farmacˆutica (TecBioFar), Programa de P´ s-graduacao em Ciˆncias o e o ¸˜ e Farmacˆuticas (PPgCF), Universidade Federal do Rio Grande do Norte (UFRN), Rua General Cordeiro de Farias, s/n, e Petr´ polis, 59012-570 Natal, RN, Brazil o 2 Laborat´rio de Farmacognosia, Departamento de Farm´ cia, Universidade Federal do Rio Grande do Norte (UFRN), o a Rua General Cordeiro de Farias, s/n, Petr´ polis, 59012-570 Natal, RN, Brazil o Correspondence should be addressed to Matheus de Freitas Fernandes-Pedrosa; mpedrosa31@uol.com.br Received 24 February 2014; Revised 1 May 2014; Accepted 1 May 2014; Published 5 June 2014 Academic Editor: Shi-Biao Wu Copyright © 2014 Juliana F´lix-Silva et al. This is an open access article distributed under the Creative Commons Attribution e License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Jatropha gossypiifolia L. (Euphorbiaceae), widely known as “bellyache bush,” is a medicinal plant largely used throughout Africa and America. Several human and veterinary uses in traditional medicine are described for different parts and preparations based on this plant. However, critical reviews discussing emphatically its medicinal value are missing. This review aims to provide an up-to-date overview of the traditional uses, as well as the phytochemistry, pharmacology, and toxicity data of J. gossypiifolia species, in view of discussing its medicinal value and potential application in complementary and alternative medicine. Pharmacological studies have demonstrated significant action of different extracts and/or isolated compounds as antimicrobial, anti-inflammatory, antidiarrheal, antihypertensive, and anticancer agents, among others, supporting some of its popular uses. No clinical trial has been detected to date. Further studies are necessary to assay important folk uses, as well as to find new bioactive molecules with pharmacological relevance based on the popular claims. Toxicological studies associated with phytochemical analysis are important to understand the eventual toxic effects that could reduce its medicinal value. The present review provides insights for future research aiming for both ethnopharmacological validation of its popular use and its exploration as a new source of herbal drugs and/or bioactive natural products.

1. Introduction
The Euphorbiaceae family, which is considered one of the largest families of Angiosperms, covers about 7,800 species distributed in approximately 300 genera and 5 subfamilies worldwide. These species occur preferentially in tropical and subtropical environments [1, 2]. Among the main genera belonging to this family, there is Jatropha L., which belongs to the subfamily Crotonoideae, Jatropheae tribe and is represented by about 200 species. This genus is widely distributed in tropical and subtropical regions of Africa and the Americas [1]. The name “Jatropha”

is derived from the Greek words “jatros,” which means “doctor” and “trophe,” meaning “food,” which is associated with its medicinal uses [3]. The Jatropha genus is divided into two subgenera, Jatropha and curcas, from which the subgenus Jatropha has the widest distribution, with species found in Africa, India, South America, West Indies, Central America, and the Caribbean [4]. Jatropha species are used in traditional medicine to cure various ailments in Africa, Asia, and Latin America or as ornamental plants and energy crops [3]. Several known species from genus Jatropha have been reported for their medicinal uses, chemical constituents, and biological activities such as Jatropha curcas, Jatropha elliptica,

2 Jatropha gossypiifolia, and Jatropha mollissima, among others [3]. From these species, Jatropha gossypiifolia L. (Figure 1) is discussed here. It is a vegetal species widely known as “bellyache bush” and is a multipurpose medicinal plant largely used in folk medicine for the treatment of various diseases [3, 5, 6]. It is widely distributed in countries of tropical, subtropical, and dry tropical weather and tropical semiarid regions of Africa and the Americas [7]. In Brazil, it predominates in the Amazon, Caatinga, and Atlantic Forest and is distributed throughout the country in the North, Northeast, Midwest, South, and Southeast regions [8]. Several human and veterinary uses in traditional medicine are described for different parts (leaves, stems, roots, seeds, and latex) and preparations (infusion, decoction, and maceration, among others) based on this plant, by different routes (oral or topical). The most frequent reports concern its antihypertensive, anti-inflammatory, antiophidian, analgesic, antipyretic, antimicrobial, healing, antianemic, antidiabetic, and antihemorrhagic activities, among many other examples [3, 5, 7, 9]. Other uses are also related to this plant, such as biodiesel production, pesticide, insecticide, vermifuge, ornamentation, and even its use in religious rituals [3, 6, 10–13]. An important feature of J. gossypiifolia species is that, due to its important potential medicinal applications, in Brazil, it is included in the National List of Medicinal Plants of Interest to the Brazilian Public Health System (Relacao Nacional de ¸˜ ´ u Plantas Medicinais de Interesse ao Sistema Unico de Sa´ de Brasileiro—RENISUS), which is a report published by the Brazilian Health Ministry in February 2009 that includes 71 species of medicinal plants that have the potential to generate pharmaceutical products of interest to public health of Brazil [14]. Regarding its phytochemical constitution, alkaloids, coumarins, flavonoids, lignoids, phenols, saponins, steroids, tannins, and terpenoids were already detected in different extracts from different parts of this plant [15]. Among the main activities already studied for this species (including various types of extracts from different parts of the plant), the antihypertensive, antimicrobial, anti-inflammatory, antioxidant, and antineoplasic activities mainly stand out, supporting some of its popular uses [3, 16]. Some toxicity studies have shown that despite the known toxicity of Jatropha species, J. gossypiifolia presented low toxicity in some in vitro and in vivo experiments. However, some studies have indicated that ethanolic extract from the leaves, in acute oral use, is safe for rats, but with chronic use, it could be toxic [17–19]. So, in view of the potential applications of this plant, this review aims to provide an up-to-date overview of the traditional uses, phytochemistry, pharmacology, and toxicity data of different parts from J. gossypiifolia, which could be significant in providing insights for present and future research aimed at both ethnopharmacological validation of its popular use, as well as its exploration as a new source of herbal drugs and/or bioactive natural products. The medicinal value and pharmacological and/or biotechnological potential of this species are also discussed in this paper.

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2. Methodology
An extensive review of the literature was undertaken in different national and international scientific sources, such as Centre for Reviews and Dissemination (http://www.crd .york.ac.uk/CRDWeb/), The Cochrane Library (http://www .thecochranelibrary.com), PubMed (http://www.ncbi.nlm .nih.gov/pubmed/), Science Direct (http://www.sciencedirect.com/), Scopus (http://www.scopus.com/), Lilacs (http://lilacs.bvsalud.org/), Scielo (http://www.scielo.org/ php/index.php), Web of Knowledge (http://apps.webofknowledge.com), and the Brazilian database of thesis and dissertations “Dom´nio P´ blico” (http://www.dominiopubliı u co.gov.br/pesquisa/PesquisaPeriodicoForm.jsp). The study database included original articles, theses, books, and other reports that preferentially had been judged for academic quality (peer-reviewed), covering several aspects of the vegetal species (botany, phytochemistry, traditional uses, pharmacology, or toxicology), dating from 1967 (first scientific report) to November 2013, without language restriction. The search strategy was constructed based on the scientific name, synonyms, and main popular names of the species identified by the botanical databases “Flora do Brasil” (http://floradobrasil.jbrj.gov.br), Tropicos (http://www.tropicos.org), The Plant List (http:// www.theplantlist.org), and NCBI Taxonomy Browser (http:// www.ncbi.nlm.nih.gov/taxonomy). The search strategy contained the combination of the following terms: “Jatropha gossypiifolia” OR “Jatropha gossypifolia” OR “Jatropha gossipyifolia” OR “Manihot gossypiifolia” OR “Adenoropium gossypiifolium” OR “Adenoropium elegans” OR “Jatropha a elegans” OR “Jatropha staphysagriifolia” OR “pinh˜ o roxo” OR “pinh˜ o-roxo” OR “pi˜ o roxo” OR “pi˜ o-roxo” OR “pe˜ oa a a a a roxo” OR “pe˜ o roxo” OR “batata-de-teu” OR “bata de teu” OR “erva-purgante” OR “erva purgante” OR “jalap˜ o” OR a “mamoninha” OR “raiz-de-tei´ ” OR “raiz de tei´ ” OR “pe˜ ou u a curador” OR “pe˜ o curador” OR “pe˜ o-paj´” OR “pe˜ o paj´” a a e a e OR “pi˜ o-caboclo” OR “pi˜ o caboclo” OR “black physicnut” a a OR “bellyache bush”. The Endnote X.3.0.1 reference manager was used. The software ACD/ChemSketch Freeware Version 12.01 was used to draw the chemical structures.

3. Botanic Information
Jatropha gossypiifolia Linneus is a Euphorbiaceae plant popularly known worldwide as “bellyache bush” or “black physicnut”. It is a pantropical species originating from South America that is cultivated in tropical countries throughout the world [20–22]. In Brazil, it is known by various popular names and the most common are “pinh˜ o-roxo,” “pi˜ o-roxo,” “pe˜ o-roxo,” a a a “batata-de-teu,” “erva-purgante,” “jalap˜ o,” “mamoninha,” a “raiz-de-tei´ ,” “pe˜ o-curador,” “pe˜ o-paj´,” “pi˜ o-caboclo,” u a a e a and “pi˜ o-preto,” among others [5, 8, 23]. There are also the a following vernacular names for J. gossypiifolia: “frailecillo,” o “frailej´ n,” “purga de fraile” (Colˆ mbia); “frailecillo” (Costa o Rica); “frailecillo,” “San Juan Del Cabre,” “t´ at´ a,” “tuba u u e tuba” (Cuba); “baga” (Malink´ et Dioula); “higuereta cimarrona,” “t´ at´ a” (Puerto Rico); “t´ at´ a” (Santo u u u u

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3

(a)

(b)

Figure 1: Jatropha gossypiifolia L. (a) aerial parts of plant. (b) flowers detail. Photography by Juliana F´lix-Silva. e

Domingo); “frailecillo,” “sibidigua,” “tuatu´ ” (Venezuela); a “pignut,” “fignut,” “lapalapa,” “binidasugu,” “oluluidi,” “botuje red,” “botuge pupa” (Nigeria); “athalai,” “lal bherenda” (´ India); “parroty grass” (Nicaragua); “babatidjin” (Africa); “pi˜ on,” “pi˜ on-colorado,” “pi˜ on negro,” “pi˜ on-rojo,” n´ n n´ n ` “purga de huane” (Spanish); “herbe a mal de ventre,” “medicinier cathartique,” “medicinier sauvage” (French); “bellyachebuhs,” “bellyache bush,” “bellyache nettlespurge,” “black physicnut,” “purge nut,” “red fig-nut flower,” “wild cassada” (English); “babatidjin,” “balautandoiong,” “cassava marble,” “cotton-leaf physicnut,” “figus nut,” “kishka,” e a e “lansi-lansinaan,” “m´dicinier bˆ tard,” “m´dicinier noir,” “m´dicinier rouge,” “quelite de fraile,” “sosori,” “tagumbau-ae nalabaga,” “tatua,” “tauataua,” “tautuba,” “tuat´ a blanca,” u “tuat´ a morada,” “tubang morado,” “tuba sa buaia,” u “tuba-tuba” (Achanti); “satamˆ n” (Bambara) [22, 24–31]. a The complete taxonomy of the species is Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; fabids; Malpighiales; Euphorbiaceae; Crotonoideae; Jatropheae; Jatropha; and Jatropha gossypiifolia [32]. Adenoropium gossypiifolium (L.) Pohl, Manihot gossypiifolia (L.) Crantz, Adenoropium elegans Pohl, Jatropha elegans Kl., Jatropha staphysagriifolia Mill., Jatropha gossypifolia, and Jatropha gossipyifolia are botanical synonymous of J. gossypiifolia species [8, 32–34]. J. gossypiifolia is a small shrub with dark green or more frequently purplish-red dark leaves, with 16–19 cm of length per 10–12.9 cm of width; they are alternate, palmate, and pubescent, with an acuminate apex, cordate base, and serrated margin. The flowers are unisexual, purple, and in cymose summits, with the calyx having five petals, which in male flowers may form a petaloid tube. The fruit is capsular, with three furrows, containing a dark seed with black spots [5, 35, 36]. Regarding the microscopic aspect of the plant leaves, some studies have shown key and important features for botanical identification of this species among other Jatropha species [21, 35–37].

reported, in general, the presence of fatty acids, sugars, alkaloids, amino acids, coumarins, steroids, flavonoids, lignans, proteins, saponins, tannins, and terpenoids, as can be seen in Table 1. Accordingly reviewed by Zhang et al. [15], the main compounds isolated from Jatropha genus are the terpenoids. In fact, many of them were isolated from different parts of J. gossypiifolia. Another very important class from J. gossypiifolia is the lignoids, since a good number of them was already isolated and identified. However, it is important to note that most of the phytochemical studies found in literature are not about isolation of compounds, but only about the phytochemical screening of the major classes through chemical qualitative reactions or more sensitive and specific methods such as thin layer chromatography (TLC). Relative to other Jatropha species, few studies have isolated chemical compounds from J. gossypiifolia (Table 2). In addition, up till now it is not clear which are the major bioactive compounds in the plant, since only a few studies were conducted by bioassay-guided isolation. Additionally, to the best of our knowledge, there are no phytochemical studies regarding the use of water as solvent for the extraction of J. gossypiifolia constituents. This is important to note since popular use occurs more frequently with infusions or decoctions, and little is known about the constitution of this type of extract. In this context, it is important to conduct studies to evaluate the phytochemical constitution of these extracts. More commonly, the studies use solvents or mixtures of solvents with nonpolar characteristics, which could contribute to further characterization of nonpolar compounds, such as terpenoids and lignoids. Polar compounds such as flavonoids, tannins, and sugars are poorly described in the species so far, probably due to this fact.

5. Traditional Uses
Various medicinal properties for the species J. gossypiifolia are reported by traditional medicine, as shown in Table 3. Some properties related to J. gossypiifolia are also common to other species of the Jatropha genus [3, 9, 25], where human and veterinary uses are described. Different parts of this plant, such as leaves, stems, roots, seeds, and latex, are used

4. Chemical Constituents
Various chemical constituents have been detected in extracts from different parts of J. gossypiifolia, the literature having

4

Table 1: Chemical constituents of Jatropha gossypiifolia L. described in the literature. Compound Propacin Venkatasin Citlalitrione Jatrophenone [38] [39] [40] [41] Reference

Plant part

Classification

Coumarin-lignoids

Whole plant

Diterpenes

Stem, roots, and seeds Coumarin-lignoids

Flavonoids Gossypiline —

Lignans

Arylnaphthalene lignan Gadain Jatrophan — Gossypifan

[42] [43] [44] [19, 45] [46] [47] [48]

Aerial parts

Phenols

Steroids Tannins Triterpenoids

Alkaloids

Cardiac glycosides

Flavonoids

Leaves

Phenols

Phlobotannins

— — — Ricinine — — — — Apigenin Isovitexin Orientin/isoorientin Schaftoside/isoschaftoside Vitexin Vitexin/isovitexin — — — — — — — —

[19, 45] [23, 45] [45] [49] [50] [51] [30] [52] [53] [53] [54] [54] [53] [54] [52] [55] [51] [55] [51] [50] [52] [56]

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Proteins

Extract type and/or preparation Isolated from dichloromethane : methanol (1 : 1, v/v) extract after successive column chromatography on silica gel Not specified∗ Isolated from dichloromethane : methanol (1 : 1, v/v) extract after successive column chromatography on silica gel Isolated from dichloromethane : methanol (1 : 1, v/v) extract after successive column chromatography on silica gel Isolated from petrol ether extract after successive column chromatography on silica gel Isolated from petrol ether extract after successive column chromatography on silica gel Isolated from petrol ether extract Detected by phytochemical screening reactions of ethanol extract Isolated from petrol ether extract after successive column chromatography on silica gel Isolated from dichloromethane : methanol (1 : 1, v/v) extract after successive column chromatography on silica gel Quantitative analysis showed that the petrol ether, chloroform, ethyl acetate, and n-butanol extracts presented, respectively, 45.0 ± 1.0, 106.0 ± 2.3, 296.0 ± 3.5, and 128.5 ± 1.1 mg of gallic acid equivalents/g of crude extract Detected by phytochemical screening reactions of ethanol extract Detected by phytochemical screening reactions of ethanol extract Detected by phytochemical screening reactions of ethanol extract Compound isolated from ethyl acetate extract from senescent leaves Detected by phytochemical screening reactions of chloroform and methanol extracts Quantitative analysis showed 2.81% on leaves Not specified Identified on leaves by qualitative phytochemical screening reactions Identified in ether fraction from ethanol extract Identified in ethyl acetate and methyl ethyl ketone fractions from ethanol extract Isomers identified in different types of extracts from leaves Isomers identified in different types of extracts from leaves Identified in ethyl acetate fraction from ethanol extract Isomers identified in different types of extracts from leaves Identified on leaves by qualitative phytochemical screening reactions Quantitative analysis showed 7.4% on leaves Quantitative analysis showed 2.41% on leaves Quantitative analysis showed 8.6% on leaves Quantitative analysis showed 0.26% on leaves Detected by phytochemical screening reactions of chloroform and methanol extracts Identified on leaves by qualitative phytochemical screening reactions Leaves obtained by micropropagation were macerated in liquid nitrogen and extracted at 4∘ C for 6 h with 0.1 M NaCl. The material was centrifuged and the limpid supernatant was dialyzed against water at low temperature in a cellulose membrane to remove nonprotein compound with molecular mass below 3.5 kDa

Table 1: Continued.

Plant part

Classification Reducing sugars

Saponins

Steroids

Tannins

Terpenoids

Reference [52] [52] [51] [52] [50] [52] [51] [52] [57] [57] [51] [58] [59] [60] [61] [62] [63] [51] [51] [51] [51] [51] [64] [64] [64] [13] [13]

Triterpenes

Alkaloids Cleomiscosin A Gossypidien Isogadain Jatrodien Prasanthaline — — — — — 2������-Hydroxyjatrophone 2������-Hydroxy-5,6-isojatrophone 2������-Hydroxyjatrophone Citlalitrione Falodone Jatropholone A Jatropholone B Jatrophone — — — —

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Compound — — — — — — — — (2������, 13������, 14������, 20S)-2,24,25Trihydroxylanost-7-en-3-one (13������, 14������, 20S)-2,24,25Trihydroxylanosta-1,7-dien-3-one — 4������ -O-Demethyl retrochinensin

Coumarin-lignoids

Stems

Flavonoids Phenols Saponins Tannins Alkaloids

Diterpenes

Roots

Flavonoids Phenols Saponins Tannins

Extract type and/or preparation Identified on leaves by qualitative phytochemical screening reactions Identified on leaves by qualitative phytochemical screening reactions Quantitative analysis showed 4.15% on leaves Identified on leaves by qualitative phytochemical screening reactions Detected by phytochemical screening reactions of methanol extract Detected on leaves by qualitative phytochemical screening reactions Quantitative analysis showed 5.14% on leaves Detected on leaves by qualitative phytochemical screening reactions Isolated from the ethanol extract after successive partitions procedures and column chromatography on silica gel and preparative TLC Isolated from the ethanol extract after successive partition procedures and column chromatography on silica gel and preparative TLC Quantitative analysis showed 2.16% of alkaloid on stems Not specified Compound isolated from ethyl acetate fraction stems after successive column chromatography on silica gel Compound isolated from hexane extract from dried stems after successive column chromatography on silica gel Not specified∗ Compound isolated from petrol ether extract after successive column chromatography on silica gel Not specified∗ Quantitative analysis showed 1.2% on stems Quantitative analysis showed 0.13% on stems Quantitative analysis showed 2.18% on stems Quantitative analysis showed 1.36% on stems Quantitative analysis showed 1.6% on roots Isolated from petrol ether extract after successive column chromatography on silica gel Isolated from petrol ether extract after successive column chromatography on silica gel Isolated from petrol ether extract after successive column chromatography on silica gel Isolated from petrol ether fraction from the methanol extract after successive column chromatography on silica gel Isolated from petrol ether fraction from the methanol extract after successive column chromatography on silica gel Not specified∗ Not specified∗ Isolated from ethanol extract Quantitative analysis showed 1.75% on roots Quantitative analysis showed 0.24% on roots Quantitative analysis showed 2.83% on roots Quantitative analysis showed 2.73% on roots

[65] [65] [66] [51] [51] [51] [51]

5

6

Table 1: Continued.

Plant part

Classification Alkaloids Amino acids

Carbohydrates 12-Deoxy-16-hydroxylphorbol

Compound — — — —

Reference [51] [67] [68] [67] [69]

Esters

Seeds

Fatty acids

Fibers Flavonoids Phenols Proteins —

Arachidic acid Caprilic acid Lauric acid Lignoceric acid Linoleic acid Myristic acid Oleic acid Palmitic acid Palmitoleic acid Ricinoleic acid Stearic acid Vernolic acid — — — —

Saponins

Tannins

Latex

Proteins

Not specified

Alkaloids

Diterpenoids

— Cyclogossine A Cyclogossine B Imidazole alkaloid Piperidine Abiodone

Extract type and/or preparation Quantitative analysis showed 2.36% on seeds Not specified∗ Quantitative analysis showed 30.32% on seeds Not specified∗ Isolated from hydrophilic fraction from the ether extract, by countercurrent chromatography Identified in petrol ether extract Identified in petrol ether extract Identified in petrol ether extract Identified in petrol ether extract Identified in petrol ether extract Identified in petrol ether extract Identified in petrol ether extract Identified in petrol ether extract Identified in petrol ether extract Identified in petrol ether extract Identified in petrol ether extract Identified in petrol ether extract Quantitative analysis showed 9.25% on seeds Quantitative analysis showed 2.26% on seeds Quantitative analysis showed 0.18% on seeds Quantitative analysis showed 13.40% on seeds Quantitative analysis showed 2.37 on seeds Quantitative analysis showed 6 g/kg on seeds Quantitative analysis showed 3.52% on seeds Not specified Isolated from ethyl acetate extract by gel filtration column chromatography Isolated from the plant exudates∗ Isolated from the plant exudates∗ Not specified∗

[68, 70] [68, 70] [68, 70] [68, 70] [68, 70] [68, 70] [68, 70] [68, 70] [68, 70] [68, 70] [68, 70] [68, 70] [68] [51] [51] [68] [51] [68] [51] [71] [20] [72] [72] [73]

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The complete version of the paper was not accessible, so the information was obtained from its abstract.

Table 2: Main isolated compounds from Jatropha gossypiifolia L. described in the literature. Plant part Biological activity Reference

Classification

Compound Ricinine O N C Leaves Insecticide in vitro

[49]

Alkaloids Not specified —

O

N
[72]

Imidazole alkaloid Piperidine Not specified —

N H
Stems

[72]

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4������ -O-demethyl retrochinensin Arylnaphthalene lignan



[58]

O OH OH

O

Stems, roots, and seeds



[42]

OMe OMe

Cleomiscosin A MeO O O
Stems — [59]

O O CH2 OH OMe

HO

7

8

Table 2: Continued. Plant part Biological activity Reference

Classification

Compound Gadain

O O H
— Stems, roots, and seeds [43]

O

O

O O COOMe O
Stems — [60]

Gossypidien

O O MeOOC O O H
Stems

O

Coumarin-lignoids

Isogadain

O

O



[61]

O O MeOOC OMe
Stems — [62]

Jatrodien

O MeOOC OMe

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O

Table 2: Continued. Plant part Biological activity Reference

Classification

Compound Jatrophan

O O H
— Stems, roots, and seeds [44]

O

O

OMe OMe OAc OAc H
Stems — [63]

Prasanthaline

O

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O

OMe OMe

Propacin MeO O O Me OMe
Whole plant — [39]

O O

Whole plant



[38]

HO

Venkatasin 2������-Hydroxyjatrophone

HO H3 C O CH3 O CH3 CH3 H3 C O

Roots

Antileukemic in vitro and in vivo

[64]

9

10

Table 2: Continued. Plant part Biological activity Reference

Classification

Compound 2������-Hydroxy-5, 6-isojatrophone

H3C HO O CH3 O CH3
Roots Antileukemic in vitro and in vivo

[64]

CH3

O

2������-Hydroxyjatrophone

H3C O
Roots

CH3
[64]

HO O CH3 CH3 O
Not specified

Antileukemic in vitro and in vivo

Diterpenes

H3C

Abiodone Citlalitrione O

Anticancer in vitro

[73]

O H

H O O

Roots



[13]

Whole plant



[40]

Falodone O
Roots Anticancer in vitro [13]

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OH

Table 2: Continued. Plant part Biological activity Reference

Classification

Compound Jatrophenone H O H H

H
Whole plant

Antibacterial in vitro

[41]

AcO H O OH
Roots —

Jatropholone A

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[65]

O

Jatropholone B OH
Roots — [65]

O O O
Roots

Jatrophone

Anticancer in vitro and in vivo

[66]

O

11

12

Table 2: Continued. Plant part Biological activity Reference

Classification

Compound 12-Deoxy-16-hydroxylphorbol

OH OH
Seeds Irritant to mouse ear

Esters

HO H

[69]

O HO O O H
Aerial parts —

OH

Gossypifan

MeO

MeO

[46]

O O

Lignans

Gossypiline OCOMe OCOMe H
Aerial parts — [47]

O

O

O O Ala2 Thr 3 Trp 4 Gly6 Leu5

Cyclogossine A
Latex — [15, 71]

Leu1

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Proteins

Val 7

Table 2: Continued. Plant part Biological activity Reference

Classification
Leu2

Compound Cyclogossine B

Gly1 Trp 3 Leu4
Latex —
Ile7

[15, 20]

Leu8 Ala6

Ala5

(13������, 14������, 20S)-2,24,25-Trihydroxylanosta-1, 7-dien-3-one HO H

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OH
Leaves — [57]

HO H

Triterpenes

O

(2������, 13������, 14������, 20S)-2,24,25-Trihydroxylanost-7-en-3-one HO H

OH
Leaves — [57]

HO H

O

13

14 in different forms of preparation (infusion, decoction, and maceration, among others), by different routes and forms (oral, topical, baths, etc.). The most frequent reports refer to its anti-inflammatory, antidiarrheal, antiophidian, analgesic, antipyretic, antimicrobial, healing, antianemic, antidiabetic, and antihemorrhagic activities, among many other examples [3, 5, 7, 9]. Some properties are attributed to specific parts of the plant, while others are assigned to different parts. Interestingly, in some cases certain uses may appear contradictory, such as antidiarrheal and laxative or its use as anticoagulant and antihemorrhagic. One hypothesis is that this difference may be related with the dose used, since, for example, the laxative effect is an effect commonly related with toxic events with this plant.

Evidence-Based Complementary and Alternative Medicine at 500 and 1000 mg/kg oral doses, was able to inhibit the acute carrageenan-induced paw edema in rats and at 50 and 100 mg/kg oral doses inhibited the chronic cotton pelletinduced granuloma formation in rats. Additionally, the J. gossypiifolia leaf paste (0.5 and 1 mg/ear) showed significant reduction in TPA-induced local inflammatory changes in mouse ear edema model [28]. In another study, the anti-inflammatory and analgesic properties of the methanol and petrol ether extracts of aerial parts of J. gossypiifolia were demonstrated in mice [92]. At 100 and 200 mg/kg/day, during 7 days, by oral route, only the methanol extract presented significant analgesic activity in Eddy’s hot plate and tail-flick models and antiinflammatory activity in carrageenan-induced paw edema [92]. The anti-inflammatory activity of the bark from J. gossypiifolia (methanol and petrol ether extracts) was also demonstrated in carrageenan-induced paw edema in rats [115]. In a recent study, using the in vitro human red blood cell membrane stabilization method, Nagaharika et al. [118] suggested that ethanol and water extracts from J. gossypiifolia leaves have anti-inflammatory activity. According to the authors, since human red blood cell membranes are similar to the lysosomal membrane components, the prevention of hypotonicity-induced membrane lysis of these cells could be taken as a measure in estimating the anti-inflammatory property of compounds [118]. The analgesic activity of the methanol extract from the leaves of J. gossypiifolia was evaluated in acetic acid-induced writhing test in mice, where highly significant inhibition was seen of 67.56 and 65.14% at 200 and 400 mg/kg oral doses, respectively [111]. Similar results were observed in the methanolic extract from fruits [110]. 6.4. Healing Action. The healing action of the ethanol crude extract of J. gossypiifolia (plant part not specified) was evaluated in suture healing of ventral abdominal wall of rats, through tensiometric measurement and macro- and microscopic aspect of postoperative period. The extract, which was administered by an intraperitoneal instillation of 100 mg/kg single dose in the peritoneal cavity, presented more intense adhesion on macroscopic examination and greater strain evaluation and vascular neoformation. However, a greater inflammatory process was also observed, and other histological parameters were similar to the control group, indicating that, in general, the extract presented poor wound healing properties in the used model [124]. Another study evaluated the healing action of the hydroethanolic crude extract from leaves of J. gossypiifolia in the healing process of sutures performed on the bladder of rats, and similar results were presented, although some improvement might have been observed in some parameters. In general, the authors concluded that no favorable healing effect was observed with the administration of single intraperitoneal dose of J. gossypiifolia L. [108]. In another study analyzing the morphological aspects of the healing process occurring in open skin lesions in rats under topical administration of raw extract from J. gossypiifolia (details

6. Pharmacological Activities
Despite the grand variety of popular uses and the data from Jatropha species, J. gossypiifolia has been scarcely studied regarding biological activities (Table 4). Studies showing the biological potential of aqueous extract are rare so far, which is important to be mentioned since the most popular use of this plant is as a tea (decoction or infusion). Among the main activities that have been studied the antihypertensive, anticancer, antimicrobial, healing, anti-inflammatory, and analgesic activities stand out. 6.1. Antihypertensive Action. Based on popular use of teas from J. gossypiifolia roots and aerial parts, the hypotensive and vasorelaxant effects of the ethanolic extract of aerial parts of the plant were tested by Abreu et al. [45]. The study revealed that the extract (125 and 250 mg/kg/day, over 4 weeks, by oral route in rats), in a dose-dependent manner, produced a reduction of systolic blood pressure in conscious normotensive animals. This hypotensive effect could be attributed to its vasorelaxant action, since it produced concentration-dependent relaxant effect in rat isolated endothelium-deprived mesenteric artery precontracted with norepinephrine or calcium. Moreover, it inhibited, in a concentration-dependent and noncompetitive manner, the contractile response induced by norepinephrine or CaCl2 in the same preparation [45]. 6.2. Antimicrobial Action. The antibiotic activity of different extracts from J. gossypiifolia is frequently reported, as observed in Table 4. In general, some extent of antibacterial, antifungal, antiparasitic, and antiviral activity was observed. The only report of J. gossypiifolia isolated compound with antimicrobial activity is of the macrocyclic diterpene jatrophenone, which presented significant in vitro antibacterial activity against Staphylococcus aureus [41]. 6.3. Anti-Inflammatory and/or Analgesic Action. Many important popular uses of J. gossypiifolia are related to inflammatory process. Bhagat et al. [28] showed that the methanolic extract of leaves of this species has significant systemic acute and chronic anti-inflammatory activity. The extract,

Table 3: Popular medicinal uses of Jatropha gossypiifolia L. described in the literature. Reference [5]

Plant part

Popular use

Analgesic (headache)

Whole plant

Aerial parts

Analgesic (toothache) Antimicrobial Antipyretic Dyscrasia Dysphonia Wound healing Antianemic (malaria treatment) Abscess Alopecia Analgesic (eye pain) Analgesic (headache) Analgesic (headache and otitis) Analgesic (pain in general) Analgesic (toothache)

Evidence-Based Complementary and Alternative Medicine

Antianemic

[3] [3] [20] [3] [3] [74, 75] [76] [77] [25] [78] [78] [79] [80] [80] [81] [82] [25] [29]

Anticancer

Anticonvulsivant

Antidiabetic

Antidiarrheal

Antihemorrhagic

Anti-infective

Anti-inflammatory

Antipyretic

Antiseptic Antithrombotic

Antiulcerogenic

Boils

Preparation and/or mode of use Leaves anointed with “Sebo de Holanda” (mutton tallow) and heated in the fire are used as compress for headaches Not specified Not specified Decoction Not specified Not specified Not specified Decoction, used by oral route Bath Ash leaves Not specified Not specified Not specified Decoction or infusion Decoction or infusion Decoction Decoction by oral route Ash of leaves Decoction of the association of leaves of J. gossypiifolia with leaves of Petiveria alliacea and aerial parts of Stachytarpheta jamaicensis, by oral route Not specified Not specified Decoction Decoction by oral route Decoction by oral route Not specified Decoction by oral route Fresh crushed leaves are used in cases of cutaneous and nasal bleeding Decoction by oral route Not specified Not specified Decoction “Tea” Not specified Bath prepared from the leaves Decoction or infusion Decoction by oral route Leaf juice Application of the pounded leaves

[3] [83] [84, 85] [30] [30] [3] [30] [86] [30] [87, 88] [78] [81] [5] [88] [5] [80] [30] [89] [90]

15

16

Table 3: Continued. Reference [25] [29]

Plant part

Popular use

Burns

Contraceptive and oxitotoxic Depurative Detoxificant Eczema Emetic Gastrointestinal disorders Gingivitis Gonorrhoea

Healing

[79] [91] [92] [25] [91] [79] [89] [25] [5] [30] [80] [93] [80] [12] [90] [89] [81] [82] [94] [94]

Hemorrhoids

Hemostatic Hepatitis Itching skin Leprosy

Leaves

Malaria

Mastitis Mycosis Psychoactive Purgative Rheumatism Scabies Skin diseases

Stomachic

Preparation and/or mode of use Ash of leaves Used in association with seeds of Gossypium arboreum, sugar, honey bee, and fat of ram, prepared by grinding, applied topically Not specified Squeezed, the juice obtained is drunk Not specified Ash of leaves Squeezed, the juice obtained is drunk Not specified Leaf juice Ash of leaves Bath prepared from the leaves Decoction Decoction or infusion Used in association with leaves of Nicotiana tabacum and copper sulphate, boiled in water, and used as steam directed at the anal region Decoction or infusion Not specified Application of the pounded leaves Leaf juice Decoction Decoction by oral route Used in association with leaves of Azadirachta indica and Combretum sp., boiled, for steam baths and by oral route Used in association with leaves of Combretum ghasalense and whole plant of Ocimum canum, by oral route or for steam baths Pounded leaves applied on swollen breasts Ash of leaves Not specified Not specified Ash of leaves Ash of leaves Not specified Decoction by oral route Not specified Ash of leaves Ash of leaves [90] [25] [79] [3, 88] [25] [25] [3] [30] [88, 92] [25] [25] Not specified Slightly boiled, used as vaginal wash Not specified Ash of leaves Not specified [79] [91] [92] [25] [3]

Evidence-Based Complementary and Alternative Medicine

Syphilis Thrush (oral candidiasis) Treatment of “cultural syndromes,” “derrame,” “quebrante,” “espante,” “vento-ca´do,” “panema,” ı “doenca-do-ar,” “m˜ e-do-corpo” ¸ a Vaginal infection Veneral diseases Vermifuge Vertigo

Table 3: Continued.

Plant part

Popular use

Wounds and rashes

Stem

Wound disinfectant Analgesic (toothache) Antianemic Anticancer Emmenagogue Malaria Rheumatism Thick blood

Anticancer

Anticonvulsivant Antidiarrheal Antimicrobial

Reference [24] [30] [95] [91] [96] [82] [26] [70, 92] [82] [77] [77] [26] [73] [83] [89] [73]

Evidence-Based Complementary and Alternative Medicine

Roots

Impotence

[29]

Seeds

Preparation and/or mode of use Bath of the leaves Decoction by oral route Decoction used as baths for cleaning wounds in dogs Slightly boiled, used as wound wash Not specified Decoction by oral route Decoction by oral or topical route Decoction of barks Decoction by oral route Not specified Not specified Decoction by oral or topical route Root bark used for cancer of the lungs Not specified Not specified Root bark used in bacterial infections Decoction of the association of roots of J. gossypiifolia, Chiococca alba, Citrus aurantifolia, Desmodium canum, Roystonea regia, Senna occidentalis, Stachytarpheta jamaicensis, and Waltheria indica with the whole plant of Commelina erecta, Cyperus rotundus, and sugar, by oral route Not specified Not specified Not specified Decoction by oral route Not specified Not specified Used in strong colds Not specified Seed oil Not specified Not specified Not specified Not specified Seed oil Seed oil Not specified Not specified Not specified Slightly boiled, used as vaginal wash Slightly boiled, used as wound wash [79] [91] [91]

Leprosy Snakebites Urinary pain Uterus diseases Analgesic (body pain) Analgesic (headache) Antigripal Antihemorrhagic Antiulcerogenic Contraceptive and oxitotoxic Depurative Emetic Gastrointestinal disorders Leprosy Mycosis Psychoactive Purgative Treatment of “cultural syndromes,” “derrame,” “quebrante,” “espante,” “vento-ca´do,” “panema,” ı “doenca-do-ar,” “m˜ e-do-corpo” ¸ a Vaginal infection Wound infection

[3, 92] [22, 92, 97, 98] [92] [99, 100] [101] [79] [5] [9] [3] [79] [91] [70, 91, 101] [79] [3] [3] [79] [3, 9, 101, 102]

17

18

Table 3: Continued. Reference [77]

Plant part

Popular use

Analgesic

Fruits

Analgesic (headache) Analgesic (toothache) Laxative Numbness after bug stings Alopecia Analgesic (eye pain) Analgesic (pain in general)

Analgesic (toothache)

Latex

Anticancer Antihemorrhagic Antithrombotic Antiulcerogenic Bite of venomous animals Diuretic Eczema Gingivitis Gonorrhea Hemostatic Infected wounds Leprosy Mycosis

Purgative

Rheumatism Scabies

Skin burns

Stop of itching of cuts and scratches Syphilis Thrush (oral candidiasis) Vermifuge

Wound healing

Resin

Evidence-Based Complementary and Alternative Medicine

Oil

Toothache Wounds in lips and tongue Arthritis Purgative Skin disease

Preparation and/or mode of use Massaging pregnant women’s bellies with tea or garrafada∗ when they are in pain Tea or garrafada∗ Tea or garrafada∗ Ingestion in natura of the powder fruit Tea or garrafada∗ Not specified Not specified Drink or massage the affected area with latex Cotton soaked with latex kept in contact with the sore tooth Drink or massage the affected area with latex Not specified Not specified Oral route Not specified Application of fresh latex at the affected site A few drops of fresh latex in water Not specified Not specified Not specified Not specified Application of fresh latex at the affected site Not specified Not specified A few drops of fresh latex in water Not specified Not specified Not specified Application of fresh latex at the affected site Not specified Not specified Not specified Not specified Not specified Application of latex at the affected site Drink or massage the affected site with latex Not specified Toothpowder Topical application Applied locally Not specified Applied locally

[77] [77] [102] [77] [25] [78] [80] [103] [80] [25] [9, 24, 86, 95] [80] [20, 89] [5] [6] [25] [89] [25] [25, 80] [5, 20] [89] [25] [6] [9] [25] [25] [104] [25] [95] [25] [25] [25] [5] [80] [74, 75] [27] [27] [89] [89] [89]

Table 3: Continued.

Plant part

Popular use Alopecia

Evidence-Based Complementary and Alternative Medicine

Analgesic

Not specified

Anticancer Antidiarrheal Antihypertensive Anti-inflammatory Antipyretic Antiseptic Antiulcerogenic Coughs and colds Detoxication Diuretic Eczema Gum infection Healing Hydropsy Leprosy Obstructions of the abdominal tract Purgative Regulate menses Rheumatism Snake and scorpion bites Stomach pain Venereal diseases

Wounds

Preparation and/or mode of use Tea applied locally in dogs Not specified Poultices Not specified Not specified Not specified Not specified Not specified Not specified Not specified Bark juice (4 spoonfuls, 3 times a day) by oral route Not specified Not specified Not specified Not specified Not specified Not specified Not specified Not specified Not specified Not specified Not specified Not specified Not specified Not specified Poultices Used as bath

Reference [105] [13] [95] [13, 66] [28, 45, 106] [45] [13, 28] [28] [45] [28] [107] [28] [45] [28] [28] [45, 108] [5] [28] [5] [5] [109] [5] [3, 22] [28] [28] [95] [28]



Garrafada: preparation done by macerating plant parts in alcohol or hydroalcoholic mediums, in general, brandies.

19

20

Table 4: Pharmacological studies of Jatropha gossypiifolia L. described in the literature. Extract/compounds Methanol and petrol ether extracts Methanol extract Methanol extract Crude latex Not specified [92] [110] [111] [112] [113] Reference

Pharmacological activity

Plant part

Aerial parts

Analgesic

Fruits

Leaves

Latex

Latex

[52]

Antibacterial

Leaves

Fractions obtained by sequential extraction of the vegetal material with petrol ether, benzene, chloroform, acetone, ethanol, methanol, and water

Detail At 100 and 200 mg/kg/day, over 7 days, by oral route in mice, only the methanol extract presented significant analgesic activity in Eddy’s hot plate and tail-flack models At 200 and 400 mg/kg, by oral route in mice, highly significantly inhibited the writhing responses induced by acetic acid At 200 and 400 mg/kg, by oral route in mice, significantly inhibited the writhing responses induced by acetic acid At 100 ������L volume inhibited in vitro Listeria monocytogenes, Salmonella tyhimurium, Salmonella typhi, and Staphylococcus aureus Presented bactericidal effect in vitro against Shigella dysenteriae and Staphylococcus aureus∗ Petrol ether fraction was inactive against Escherichia coli and Bacillus subtilis. Benzene fraction was the most active, against both microorganisms. Chloroform and methanol fractions were active only against Bacillus subtilis. Acetone and ethanol fractions were active only against Escherichia coli. Aqueous fraction was active against both microorganisms, although to a much lesser degree than the other fractions. All extracts were active in vitro against Shigella dysenteriae∗ [113]

Methanol, chloroform, and water extracts Petrol ether and ethyl acetate fractions from ethanol : dichloromethane (1 : 1, v/v) extract Jatrophenone Water and ethyl acetate fractions from methanol extract

[87]

Whole plant

[41] [114]

Aerial parts

Leaves

Chloroform extract

[50]

Dichloromethane : methanol (1 : 1, v/v) extract

[88]

Leaves Methanol extract

The petrol ether fraction (1 mg/mL) inhibited in vitro Pseudomonas aeruginosa, Staphylococcus epidermidis, and Salmonella typhimurium. The ethyl acetate fraction (1 mg/mL) was active against Staphylococcus aureus Presented in vitro antibacterial activity against Staphylococcus aureus comparable to penicillin Both fractions, at 1 mg, did not produce zones of inhibition for Escherichia coli, Staphylococcus aureus, Saccharomyces cerevisiae,nor Candida albicans Presented antibacterial activity against Salmonella typhi, Pseudomonas aeruginosa,,and Staphylococcus aureus and antifungal activity against Candida albicans. Did not produce inhibition zones against Escherichia coli, Bacillus subtilis, Proteus mirabilis, Corynebacterium diptheriae, Shigella dysenteriae,and Streptococcus penumoniae At 0.5 and 1 mg/mL, showed significant antibacterial activity in vitro against Bacillus cereus var mycoides, Bacillus pumilus, Bacillus subtilis, Bordetella bronchiseptica, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis, Klebsiella pneumoniae, and Streptococcus faecalis and antifungal activity in vitro against Candida albicans Presented antibacterial activity against Salmonella typhi, Pseudomonas aeruginosa, and Staphylococcus aureus and antifungal activity against Candida albicans. Did not produce inhibition zones against Escherichia coli, Bacillus subtilis, Proteus mirabilis, Corynebacterium diptheriae, Shigella dysenteriae,and Streptococcus penumoniae

Evidence-Based Complementary and Alternative Medicine

[50]

Table 4: Continued. Extract/compounds Reference

Pharmacological activity

Plant part

Evidence-Based Complementary and Alternative Medicine

Antibacterial and antifungal

Not specified

Extracts obtained by sequential extraction of the vegetal material with n-hexane, chloroform, acetone, methanol, and water

[89]

Methanol and petrol ether extracts from bark

[115]

Latex

Lyophilized latex

Detail n-Hexane extract had inhibitory activity in vitro against Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Bacillus cereus, Klebsiella aerogenes, and Candida albicans but was inactive against Shiguella boydi, Aspergillus fumigatus, Aspergillus flavus, and Aspergillus niger. Chloroform extract inhibited in vitro Salmonella typhi, Pseudomonas aeruginosa, Bacillus cereus,and Candida albicans but was inactive against Escherichia coli, Staphylococcus aureus, Shiguella boydi, Aspergillus fumigatus, Aspergillus flavus, and Aspergillus niger. Acetone extract inhibited in vitro Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella aerogenes, Proteus vulgaris,and Candida albicans but was inactive against Salmonella typhi, Aspergillus fumigatus, Aspergillus flavus,and Aspergillus niger. Methanol extract inhibited in vitro Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus,and Candida albicans but was inactive against Aspergillus fumigatus, Aspergillus flavus,and Aspergillus niger. Water extract was active in vitro against Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, Klebsiella aerogenes, Proteus vulgaris,and Candida albicans but was inactive against Aspergillus fumigatus, Aspergillus flavus, and Aspergillus niger At 200 ������g/100 ������L, only the methanol extract showed in vitro antibacterial activity upon Staphylococcus aureus, Streptococcus pyogenes, and Escherichia coli and antifungal activity upon Aspergillus niger, Candida albicans, Penicillium notatum, and Saccharomyces cerevisiae Inhibited time- and dose-dependently the acetylcholinesterase enzyme in nervous tissue of freshwater air breathing fish Channa marulius At 2 mg/mL concentration, the ethyl acetate and methanol fractions presented inhibitory activities in vitro of 71 and 100%. The methanol fraction presented IC50 of 0.05 mg/mL

[116]

Anticholinesterase Fractions obtained by sequential extraction of the vegetal material with ethyl acetate and methanol Methanol extract Methanol extract

Leaves

[117]

Fruits

[110] [111]

Antidiarrheal

Leaves

Antifungal (antidermatophytic fungi)

Aerial parts

Water and ethyl acetate fractions from methanol extract

At 200 and 400 mg/kg, by oral route in mice, inhibited the castor oil induced diarrhea At 200 and 400 mg/kg, by oral route in mice, inhibited the castor oil induced diarrhea The minimal concentration producing 75% of inhibition or higher against Microsporus canis, for both fractions, was 1 ������g/mL. For the fungus Microsporus gypseum, Microsporus fulvum,and Microsporus gallinae, none of the fraction presented inhibitory activity

[114]

21

22

Table 4: Continued. Extract/compounds Methanol and petrol ether extracts Aqueous extract Ethanol extract [92] [118] [118] Reference

Pharmacological activity

Plant part

Aerial parts

Leaves

Anti-inflammatory Methanol extract and leaf paste

[28]

Not specified

Antimalarial Dichloromethane extract

Leaves

Methanol and petrol ether extracts from bark Aqueous extract

[115] [31] [81]

Ethanolic extract and jatrophone Falodone

[66]

Antineoplasic

Roots

Detail At 100 and 200 mg/kg/day, over 7 days, by oral route in mice, only the methanol extract presented significant anti-inflammatory activity on carrageenan-induced paw edema At 100 and 200 ������g/mL, significantly prevented the lysis of human red blood cells in membrane stabilization method in vitro At 100 ������g/mL, significantly prevented the lysis of human red blood cells in membrane stabilization method in vitro At 500 and 1000 mg/kg, by oral route in rats, inhibited the carrageenan-induced paw edema. At 50 and 100 mg/kg, by oral route in rats, inhibited the cotton pellet induced granuloma formation in rats. At 0.5 and 1 mg/ear, the leaf paste reduced the inflammation response in mouse ear edema model At 200 mg/kg, by oral route in rats, both extracts reduced the carrageenan-induced paw edema 30 ������g inhibited in vitro the growth of Plasmodium falciparum Active in vitro against Plasmodium falciparum, with IC50 of about 35 ������g/mL The ethanol extract, as well as jatrophone, exhibited significant inhibitory activity in vitro against cells derived from human carcinoma of the nasopharynx and lymphocytic leukemia P-388 and in vivo against four standard animal tumor systems Showed potent proliferation inhibitory activity against A-549 human cancer cell line, with IC50 of 120 ������g/mL [13] Presented antineoplasic activity upon P-388 lymphocytic leukemia test system both in vivo and in vitro, as well as for the Eagle’s carcinoma of the nasopharynx test system in vitro [64]

Not specified

[73] [55]

Leaves

2������-Hydroxyjatrophone, 2������hydroxy-5,6-isojatrophone and 2������-hydroxyjatrophone, diterpenes isolated from petrol ether extract Abiodone Methanol, ethyl acetate, and aqueous extract

Antioxidant

Whole plant

Petrol ether, chloroform, ethyl acetate, and n-butanol extracts

[48]

Evidence-Based Complementary and Alternative Medicine

Antispasmodic

Aerial parts

Ethanol extract, fractions, and subfractions

Not specified∗ All extracts showed significant antioxidant activity in vitro in DPPH free radical, ferric thiocyanate, and nitric oxide scavenging methods∗ All extracts showed only poor DPPH scavenging activity. The total antioxidant capacity was higher in ethyl acetate and n-butanol extracts, having the petrol ether and chloroform showing only poor activity. The lipid peroxidation was inhibited only partially by the extracts, with the ethyl acetate being the most active and the petrol ether being the least At 500, 1000, and 2000 mg/kg, by oral route in mice, showed significant antispasmodic activity in mouse intestinal transit model and at 0.5, 1.0, and 2.0 mg/mL inhibited in vitro the acetylcholine and calcium-induced contractions of isolated rat jejunum. Only the organic fraction of the extract had a calcium-antagonist effect, whereas both chloroformic and aqueous fractions had anticholinergic effect

[119]

Table 4: Continued. Extract/compounds Reference

Pharmacological activity

Plant part

Antiviral

Aerial parts

Water and ethyl acetate fractions from methanol extract

[114]

Not specified Aqueous extract Ethanol extract Ethanol 70% extract Ethanol 70% extract

Methanol extract from barks

[107] [120] [121] [122] [123]

Bronchodilator

Stems

Contraceptive

Leaves

Aerial parts

Evidence-Based Complementary and Alternative Medicine

Aerial parts

Healing

Leaves

Hydroethanol extract

[108]

Not specified

Ethanolic extract

[124]

Not specified Crude fresh latex Petrol ether, methanol, and water extracts

[125] [101] [126]

Hemostatic

Latex

Hepatoprotective

Aerial parts

Hypotensive and vasorelaxant

Aerial parts

Ethanolic extract

[45]

Immunomodulatory

Whole plant

Petrol ether extract Methanol and aqueous extracts

[127] [128]

Local anesthetic

Not specified

Detail At 1, 10, and 100 ������g/mL, both fractions presented 100% of inhibition of plaque-forming ability of Sindbis virus in treatment preinfection protocol (IC50 < 1 ������g/mL), while in treatment postinfection, the IC50 of water fraction increased to 512 and acetate fraction increased to 37 ������g/mL. For murine cytomegalovirus, IC50 of 1.7 and 1.5 to water and ethyl acetate fractions were observed, respectively, in treatment preinfection protocol. In the treatment postinfection, however, no inhibition was observed in this microorganism Partially active against Sindbis virus and herpes simplex virus-l. Inactive against human poliovirus The extract was inactive in bronchodilator activity in guinea pigs At 450 mg/kg/day, over 21 days, by oral route, caused an antifertility activity in female mice At 1 mL/kg dose, by intraperitoneal route in rats, presented beneficial activity in healing process of colonic anastomosis At 200 mg/kg, by intraperitoneal route in rats, favored the healing process of gastrorrhaphies and reduced the acute inflammatory reaction in vivo At 200 mg/kg, by intraperitoneal route, decreased the inflammation and increased vascular neoformation and collagen deposition when compared to the control group in healing process of sutures performed on the bladder of rats. However, in general, no favorable healing effect was observed. Although some improvement could be observed in suture healing of ventral abdominal wall of rats treated with 100 mg/kg of extract (intraperitoneal instillation intraperitoneal cavity), in general, only a poor healing activity was observed. At 0.1 mL volume, by topical application, the crude extract presented significant differences concerning the macroscopic and microscopic aspects of healing process occurring in open skin lesions in rats Decreased clotting and bleeding time in healthy subjects At 200 mg/kg/day, over 7 days, by oral route in rats, both extracts presented hepatoprotective activity in carbon tetrachloride induced liver damage, with the petrol ether being the most active and the methanol being the least At 125 and 250 mg/kg/day, over 4 weeks, by oral route in rats, in a dose-dependent manner, reduced the systolic blood pressure and produced a concentration-dependent relaxant effect in rat isolated (ex vivo) endothelium-deprived mesenteric artery precontracted with norepinephrine or CaCl2 At 100, 200, and 400 ������g/mL increased the proliferation of mouse spleen cell in vitro Both extracts presented significant local anesthetic activity by plexus anesthesia in frogs∗

23

24

Table 4: Continued. Extract/compounds Ethanolic extract and chloroformic and aqueous fractions Reference [129]

Pharmacological activity

Plant part

Relaxant effect on uterine smooth muscle (tocolytic activity)

Aerial parts

Fruits

Methanol extract

[110]

Sedative and anxiolytic Methanol extract

Leaves

Detail At 0.5 and 1.0 mg/mL, the ethanolic extract reduced the calcium-evoked contractile response of the uterine smooth muscle, as well as the chloroformic fraction. The aqueous fraction presented only slight effect At 200 and 400 mg/kg, by oral route in mice, presented sedative effect in the hole cross test; At 200 mg/kg, presented anxiolytic activity in hole board test; At 400 mg/kg, presented anxiolytic activity in elevated plus-maze test At 200 and 400 mg/kg, by oral route in mice, presented sedative effect in the hole cross test; At 200 mg/kg, presented anxiolytic activity in hole board test; At 400 mg/kg, presented anxiolytic in elevated plus-maze test

[111]

Evidence-Based Complementary and Alternative Medicine

∗ The complete version of the paper was not accessible, so the information was obtained from its abstract. IC50 : concentration that inhibits 50% of the referred activity.

Evidence-Based Complementary and Alternative Medicine about extract preparation and plant part not specified), the authors also observed an absence of healing action, although some histological improvement was shown [125]. However, studying the influence of J. gossypiifolia on the healing process of colonic anastomosis in rats, Servin et al. showed that the administration of 1 mL/kg single dose of the hydro alcoholic extract from aerial parts has beneficial effect on the healing process [122]. However, according to these authors, on the seventh day of the experiment, there was a decrease in the action of the extract, suggesting that the extract, in this experiment, was less active in later stages of healing process [122]. A plausible hypothesis, not raised by the authors, could be the fact that the extract was administered in a single dose, which may not have been sufficient to maintain the effect throughout the time of the experiment. Additionally, Vale et al. showed that the ethanolic extract from aerial parts of J. gossypiifolia, at single intraperitoneal dose of 200 mg/kg, favored the healing process of gastrorrhaphies and reduced the acute inflammatory reaction in vivo [123]. 6.5. Hemostatic Action. The use of J. gossypiifolia, especially the latex, is widespread as a hemostatic agent for preventing bleeding disorders. The results of whole blood clotting time using Lee and White method and bleeding time using Ivy’s method were significantly reduced when stem latex was introduced, suggesting procoagulant activity [101]. Regarding the possible mechanism of action, based on experiments that show the precipitating action of the latex upon bovine albumin, the authors suggest that the latex precipitates clotting factors thereby bringing the coagulation factors into close contact, and then the activation of coagulation cascade leads to the generation of thrombin and formation of a clot takes place in a matter of seconds when compared to the control experiment, which took minutes to complete coagulation [101]. It is important to emphasize that, to the best of our knowledge, this is the only study performed on human subjects. 6.6. Anticholinesterase Action. Based on the cholinergic hypothesis, acetylcholinesterase inhibitors are widely used to treat Alzheimer’s disease. J. gossypiifolia presented an important anticholinesterase activity since the methanolic extract from leaves showed an IC50 of 0.05 mg/mL [117]. Another study showed that the lyophilized latex of the plant was able to inhibit time- and dose-dependently the acetylcholinesterase enzyme in nervous tissue of freshwater air breathing fish Channa marulius [116]. 6.7. Antioxidant Action. The antioxidant activity of extracts from J. gossypiifolia was evaluated by Kharat et al. [55]. In this work the high content of phenols, tannins, and flavonoids in the leaves prompted the authors to evaluate the antioxidant activity of the leaves. DPPH free radical, ferric thiocyanate, and nitric oxide scavenging methods were used to analyze the antioxidant activity in vitro of methanol, ethyl acetate, and aqueous extracts, demonstrating positive results. The authors attributed the free radical scavenging activity to the presence

25 of flavonoids [55]. On the other hand, a study showed that different extracts (petrol ether, chloroform, ethyl acetate, and n-butanol) from whole plant of J. gossypiifolia had only partial antioxidant activity in DPPH scavenging, total antioxidant capacity, and lipid peroxidation tests [48]. Among them, the ethyl acetate extract was the most active, which correlates positively with its higher content of phenolic compounds in comparison with the other extracts [48]. 6.8. Contraceptive Action. Based on its popular use, J. gossypiifolia was assessed for its antifertility activity, as an alternative to oral contraceptive agents. J. gossypiifolia leaf extract, by oral route, altered the major hormones involved in estrous cycle regulation, indicating its antifertility effect on mice [121]. Evaluating other parameters (estrogenic and early abortifacient activities) the anti-infertility effect of the extract was once more demonstrated later [130]. 6.9. Tocolytic Action. Based on the ethnopharmacological application of the plant as tocolytic remedy, the effects on calcium-evoked uterine smooth muscle contraction of the ethanolic extract and fractions were evaluated [129]. The crude extract and, to a higher extent, the chloroformic fraction reduced the calcium-evoked contractile response of the uterine smooth muscle, promoting a rightward displacement of calcium cumulative curves, as well as reducing the maximal contractions [129]. 6.10. Antineoplasic Action. One of the most well-known pharmacological activities of J. gossypiifolia is its antineoplasic action, which is frequently associated with the content of lignoids and terpenoids. One of the first reports was made by Kupchan et al. [66], when the authors found that the ethanolic extract from roots, as well as the isolated diterpene jatrophone, exhibited significant inhibitory activity in vitro against cells derived from human carcinoma of the nasopharynx and lymphocytic leukemia P-388 and in vivo against four standard animal tumor systems, such as sarcoma 180, Lewis lung carcinoma, P-388 lymphocytic leukemia, and Walker 256 intramuscular carcinosarcoma [66]. Later, three new antitumor derivatives of jatrophone were isolated from petrol ether extracts from roots of J. gossypiifolia: 2������hydroxyjatrophone, 2������-hydroxy-5,6-isojatrophone, and 2������hydroxyjatrophone [64]. Recently, two other diterpenes with potent antineoplasic activity were isolated from J. gossypiifolia: falodone and abiodone. Falodone was isolated from methanol extract from roots and showed potent proliferation inhibitory activity against A-549 human cancer cell line [13]. Abiodone, a lathyrane diterpenoid compound, was isolated from J. gossypiifolia and presented potent anticancer activity [73]. 6.11. Local Anesthetic Action. The local anesthetic action of J. gossypiifolia was evaluated by plexus anaesthesia in frogs [128]. The authors observed that the aqueous and methanol extract (plant part not specified) presented significant anesthetic action when compared to control group.

26 6.12. Neuropharmacological Action. The neuropharmacological action of the methanol extract of the leaves of J. gossypiifolia was evaluated by Apu et al. [111]. The authors observed that in hole cross test the extract at 200 and 400 mg/kg, by oral route, showed significant sedative effect in mice. In hole board test, the extract showed highly significant anxiolytic activity at a dose of 200 mg/kg, whereas the same activity was observed at 400 mg/kg dose in elevated plus-maze test [111]. Similar results were observed in the methanolic extract from fruits [110]. 6.13. Antidiarrheal Action. Although it may seem contradictory as shown in Table 3, J. gossypiifolia species is popularly used both as purgative and as antidiarrheal remedy. However, in literature, there are interesting results about the antidiarrheal properties of different extracts of this species. At 200 and 400 mg/kg oral doses in mice, the methanol extract of J. gossypiifolia leaves produced highly significant antidiarrheal activity upon castor oil-induced diarrhea, decreasing the mean number of stool and total weight of fecal output when compared to control group [111]. Similar results were observed in the methanolic extract from fruits [110]. Aiming to determine the possible action mechanism of J. gossypiifolia aerial parts ethanol extract as antidiarrheal agent, Silva et al. [119] have investigated the effect of this extract on intestinal transit velocity and on isolated rat jejunum. At 500, 1000, and 2000 mg/kg, by oral route in mice, the extract showed significant antispasmodic activity in mouse intestinal transit model when compared to control. At 0.5, 1.0, and 2.0 mg/mL, the crude extract inhibited in vitro the acetylcholine and calcium-induced contractions of isolated rat jejunum. The chloroform and aqueous fractions were obtained and it was observed that only the chloroform fraction of the extract had a calcium-antagonist effect, whereas both chloroformic and aqueous fractions had anticholinergic effect, suggesting that the antispasmodic effect of J. gossypiifolia may be due to a combination of anticholinergic and calcium-antagonist mechanisms [119]. 6.14. Immunomodulatory Action. The immunomodulatory action of synthetic lignan compounds was evaluated by the assay of proliferation of mouse spleen cell in vitro and compared with petrol ether extract of whole plant of J. gossypiifolia, since it is a natural source of this kind of compound [127]. The authors showed that both synthetic and naturally occurring 1-phenylnaphthalene lignans could positively modulate the immunity of the host, since they significantly increased the proliferation of mouse spleen cell in vitro [127]. 6.15. Hepatoprotective Action. Despite some studies having shown the hepatotoxic potential of J. gossypiifolia, a study was performed to analyze the possible hepatoprotective action of extracts of this plant in carbon tetrachloride-induced liver damage in rats [126]. In fact, the petrol ether, methanol, and water extracts from the aerial parts of J. gossypiifolia presented significant hepatoprotective action in this model,

Evidence-Based Complementary and Alternative Medicine substantially restoring towards normalization the serum levels of serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase, serum alkaline phosphatase, total bilirubin, superoxide dismutase, and catalase [126]. The authors also discuss the close relationship between the hepatoprotective action observed and the possible antioxidant mechanism present in the extracts.

7. Other Actions and Biotechnological Applications
In addition to studies demonstrating scientific evidences of the pharmacological properties of J. gossypiifolia, several studies have demonstrated the potential of this species to obtain molecules with various applications, thus showing its multipurpose character. Among the main applications described, the use of J. gossypiifolia seed oil for biodiesel production could be mentioned. Jatropha species has drawn the attention of researchers in recent years due to its emergence as a highly suitable feedstock plant for biodiesel production [11]. Among the species, J. gossypiifolia, J. curcas, and J. pohliana produce seeds with high oil content [11]. In a study investigating the potential of two plants of the Jatropha genus (including J. gossypiifolia), the authors observed that the studied physicochemical properties of the produced biodiesel are in the acceptable range for use as biodiesel in diesel engines, showing a promising economic exploitation of these raw materials [131]. Studies have shown the potential of the species for the development of new tools for biochemical analysis. A recent study showed that the diluted fresh latex J. gossypiifolia can be used as precipitating agent for biochemical determination of proteins in plasma, urine, and cerebrospinal fluid, with values comparable to those obtained from the conventional protein precipitants sodium tungstate and trichloroacetic acid [24]. According to the authors, the precipitating potential could be related to the capacity of the latex to form clots when applied to a bleeding sore or wound when it is used in folk medicine [24]. Another study showed the potentiality of the juice extracted from the fresh leaves of J. gossypiifolia as an anticoagulant for haematological analyses [86]. 0.1 mL of extract per mL of blood proved to be suitable for obtaining plasmas for biochemical analysis comparable with conventional anticoagulants [86]. However, the authors emphasize that the extract must be purified to remove interfering substances for it to be perfectly suitable for biochemical analysis [86]. Some studies have demonstrated the potentiality of J. gossypiifolia as a source of pesticide biomolecules. Bullangpoti et al. [49] isolated ricinine from the ethyl acetate extract from senescent leaves, the main compound responsible for the toxicity of the crude extract in Spodoptera exigua larvae, thus demonstrating that it could be an alternative choice to chemical insecticides. In another study, Bullangpoti et al. [132] showed that the ethanol extract of J. gossypiifolia in association with the ethanol extract of Melia azedarach was toxic and inhibited some enzymes from Spodoptera

Evidence-Based Complementary and Alternative Medicine frugiperda larvae, demonstrating once more the potentiality of the species as insecticide agent. Calatayud et al. [56] showed the presence of proteins of about 100 kDa with toxic activity upon Phenacoccus herreni, another type of insect. In this work, the authors performed a strategy of extraction that eliminated nonprotein compounds, being able to demonstrate the potential of the species to obtain insecticidal proteins [56]. Leaf extract of J. gossypiifolia reduced the fecundity and egg viability against stored product insect pests Tribolium castaneum [133]. The potential molluscicidal activity of J. gossypiifolia has also been evaluated as an alternative mode of prevention of schistosomiasis. Sukumaran et al. [134] showed that the methanol and n-butanol extracts from unripened seeds of J. gossypiifolia was toxic against eggs and adults of two species of freshwater snails, Lymnaea luteola and Indoplanorbis exustus. The results indicated that n-butanol extract was the most effective and that the eggs were more susceptible than adults [134].

27 by a bioguided isolation, the irritant polyunsaturated ester 12-deoxy-16-hydroxylphorbol was isolated from the ether extract from the seeds of J. gossypiifolia by countercurrent chromatography [69]. The irritant activity was visualized in mouse ear after 24 h of the application of the fractions and isolated compounds [69]. The in vitro cytotoxicity assay using brine shrimp larvae test revealed that ethanol and methanol extracts (plant organ unspecified) showed low toxicity [138]. An earlier study showed that the water and ethyl acetate fraction of a methanol extract from aerial parts of J. gossypiifolia did not present toxicity against the same organisms [114]. A study performed in Wistar rats evaluated the toxicity of the ethanolic root extract of J. gossypiifolia at 10, 20, and 30 mg/kg by oral route [139]. The authors observed that the extract was toxic to the kidney and caused increased urea retention in the blood, as observed by histological studies and biochemical analysis of blood [139]. A preclinical toxicological assessment of the crude ethanol extract from J. gossypiifolia leaves showed that the extract presents relatively low oral acute toxicity in Wistar rats [18, 19]. Rats treated with single doses of 1.2–5.0 g/kg by oral route were observed for 14 days, and the most important signs of toxicity were ptosis, reduction of body weight, and hind limb paralysis. Other significant alterations occurred only in males treated with 5.0 g/kg dose: increase in creatinine, aspartate aminotransferase, sodium and potassium seric levels, reduction of urea and albumin, leucopenia and small alteration in color, and consistency of viscera. The median lethal dose (LD50 ) was higher than 4.0 g/kg for males and higher than 5.0 g/kg for females [19]. In the histopathological evaluation some alteration was observed in liver and lung only at 5.0 g/kg, suggesting the relatively low toxicity of the extract [18]. However, in the chronic toxicological study (thirteen weeks of treatment), this extract showed significant oral chronic toxicity in rats [17]. The most significant toxic signs indicated a reduction of the activity in the central nervous system and digestive disturbances. The histopathological analysis revealed hepatotoxicity and pulmonary damages. The lethality was 46.6% and 13.3% among males and females under the higher tested dose (405 mg/kg), respectively [17]. Based on this, Mariz et al. [7] discussed that the development of herbal medicine based on this species needs to prioritize the chemical refinement of the crude extracts to obtain less toxic fractions, which should be tested for their safety and therapeutic efficacy. Another study, on the other hand, evaluating the oral acute toxicity of the aqueous and ethanol extracts from leaves of J. gossypiifolia, did not show any sign of toxicity in up to 2 g/kg in rats, enabling the authors to conclude that this extract could be considered safe [118]. This is an interesting result since in most cases the plant is used popularly as tea (aqueous extract). The toxicity of the stem latex of J. gossypiifolia was studied in Wistar rats by applying different doses of crude latex on incised skin daily for 18 days, based on the popular use of the latex as hemostatic agent in skin lesions [140]. The authors observed that the application of the latex did not produce any significant difference in results of biochemical

8. Toxicology
Species of Jatropha are notably known for their toxic potential [135, 136]. This toxicity is related primarily to latex and seeds. The latex is released from the aerial parts of the plant by mechanical injury and it is extremely caustic and irritating to skin and mucous membranes. The seeds are rich in toxalbumins that cause agglutination and hemolysis to erythrocytes as well as damage to other cell types and contain a lipoid resin complex that can cause dermatitis [3, 12, 135]. The symptomatology consists, in general, of gastrointestinal disorders (abdominal pain, nausea, vomiting, and diarrhea). Additionally, the clinical course can bring cardiovascular, neurological, and renal complications [136]. Cases of poisoning in humans usually occur by eating fruit and seeds because of its similarity to edible chestnuts [136]. Some toxicological studies have demonstrated the toxic properties of J. gossypiifolia, while others show the absence of toxicity. However, it is important to observe the models used, doses administrated, and types of extract employed (solvent and plant part), among other aspects, to make the proper conclusions about the toxicity. The study of experimental poisoning in sheep showed that the intake of fresh plant leaves in a single dose of 40 g/kg was lethal to these animals [137]. The clinical and pathological picture in the experimental sheep was characterized by digestive, lung, and heart disturbances and also by slight regressive changes evidenced in hepatic and renal histological examinations [137]. However, as observed by Mariz et al. [7], it is important to note that the medicinal use of the plant is rarely in natura, but instead by different preparations, such as infusions or decoctions, sometimes of the dried material, which could inactivate the possible toxic components. However, this is only a hypothesis, and so the toxicity of extracts from leaves cannot be discarded. One of the first studies relating the identification of the constituents responsible for the toxic effects of the Jatropha species was published by Adolf et al. [69]. In this work,

28 and hematological parameters obtained from the control and experimental animals, leading to the conclusion that the stem latex has no harmful effects [140].

Evidence-Based Complementary and Alternative Medicine
[5] L. C. Di Stasi and C. A. Hiruma-Lima, Plantas medicinais na Amazˆnia e na Mata Atlˆ ntica, UNESP, S˜o Paulo, Brazil, 2nd o a a edition, 2002. [6] M. D. F. Agra, K. N. Silva, I. J. L. D. Bas´lio, P. F. De Freitas, ı and J. M. Barbosa-Filho, “Survey of medicinal plants used in the region Northeast of Brazil,” Brazilian Journal of Pharmacognosy, vol. 18, no. 3, pp. 472–508, 2008. [7] S. R. Mariz, A. C. R. Borges, M. F. F. Melo-Diniz, and I. A. Medeiros, “Possibilidades terapˆuticas e riscos toxicol´ gicos e o de Jatropha gossypiifolia L.: uma revis˜o narrativa,” Revista a Brasileira De Plantas Medicinais, vol. 12, no. 3, pp. 346–357, 2010. [8] I. Cordeiro and R. Secco, “Jatropha gossypiifolia L. Lista de esp´cies da flora do Brasil,” Jardim Botˆ nico do Rio de Janeiro, e a http://floradobrasil.jbrj.gov.br/jabot/floradobrasil/FB17581. [9] U. P. de Albuquerque, P. M. de Medeiros, A. L. S. de Almeida et al., “Medicinal plants of the caatinga (semi-arid) vegetation of NE Brazil: a quantitative approach,” Journal of Ethnopharmacology, vol. 114, no. 3, pp. 325–354, 2007. [10] U. P. Albuquerque, L. H. C. Andrade, and J. Caballero, “Structure and floristics of homegardens in Northeastern Brazil,” Journal of Arid Environments, vol. 62, no. 3, pp. 491–506, 2005. [11] S. A. Ceasar and S. Ignacimuthu, “Applications of biotechnology and biochemical engineering for the improvement of Jatropha and Biodiesel: a review,” Renewable and Sustainable Energy Reviews, vol. 15, no. 9, pp. 5176–5185, 2011. [12] U. P. de Albuquerque, J. M. Monteiro, M. A. Ramos, and E. L. C. de Amorim, “Medicinal and magic plants from a public market in northeastern Brazil,” Journal of Ethnopharmacology, vol. 110, no. 1, pp. 76–91, 2007. [13] A. Falodun, Q. Sheng-Xiang, G. Parkinson, and S. Gibbons, “Isolation and characterization of a new anticancer diterpenoid from Jatropha gossypifolia,” Pharmaceutical Chemistry Journal, vol. 45, no. 10, pp. 636–639, 2012. [14] BRASIL, “Fitoterapia: plantas de interesse ao SUS,” Brazilian Health Ministry, http://portal.saude.gov.br/portal/arquivos/pdf/RENISUS.pdf. [15] X.-P. Zhang, M.-L. Zhang, X.-H. Su, C.-H. Huo, Y.-C. Gu, and Q.-W. Shi, “Chemical constituents of the plants from genus Jatropha,” Chemistry and Biodiversity, vol. 6, no. 12, pp. 2166– 2183, 2009. [16] S. K. Sharma and H. Singh, “A review on pharmacological significance of genus Jatropha (Euphorbiaceae),” Chinese Journal of Integrative Medicine, vol. 18, no. 11, pp. 868–880, 2012. [17] S. R. Mariz, G. S. Cerqueira, W. C. Ara´ jo et al., “Chronic toxicou logic study of the ethanolic extract of the aerial parts of Jatropha gossypiifolia in rats,” Revista Brasileira de Farmacognosia, vol. 22, no. 3, pp. 663–668, 2012. [18] S. R. Mariz, M. S. T. Araujo, G. S. Cerqueira et al., “Histopathological evaluation in rats after acute treatment with the ethanol extract from aerial parts of Jatropha gossypiifolia L,” Revista Brasileira De Farmacognosia, vol. 18, no. 2, pp. 213–216, 2008. [19] S. R. Mariz, G. S. Cerqueira, W. C. Ara´ jo et al., “Estudo toxiu col´ gico agudo do extrato etan´ lico de partes a´reas de Jatropha o o e gossypiifolia L. em ratos,” Revista Brasileira de Farmacognosia, vol. 16, no. 3, pp. 372–378, 2006. [20] C. Auvin-Guette, C. Baraguey, A. Blond, J. L. Pousset, and B. Bodo, “Cyclogossine B, a cyclic octapeptide from Jatropha gossypifolia,” Journal of Natural Products, vol. 60, pp. 1155–1157, 1997.

9. Conclusions
As demonstrated by this review, J. gossypiifolia presents an important potential for the generation of pharmacological and/or biotechnological products, based on popular uses and biological studies scientifically showing its properties. However, regarding specifically its medicinal properties, further studies are still necessary to assay important folk uses of the species and characterize the major compounds responsible for the bioactivity. Thus, studies of bioprospecting could prioritize this species, since many popular uses for various medical purposes are reported, demonstrating a great potential to originate bioactive molecules with pharmacological relevance. Furthermore, future phytochemical studies of this plant are important to obtain the best knowledge of the chemical composition of different extracts of the plant, in order to recognize the really important compounds in the pharmacological actions, aspiring to the chemical refinement of the products to eliminate the eventual toxic effects that could reduce the medicinal value of the species. In conclusion, the data presented in this review could provide insights for future research aimed at both ethnopharmacological validation of the popular use of J. gossypiifolia and its exploration as a new source of bioactive molecules for herbal drugs and/or bioactive natural products for potential application in complementary and alternative medicine.

Conflict of Interests
The authors declare that there is no conflict of interests regarding the publication of this paper.

Acknowledgments
The authors thank BNB, CNPq, and CAPES (Brazil) for the financial support. Matheus de Freitas Fernandes-Pedrosa gives thanks to CNPq for Scholarship in Research Productivity. The authors also thank Andrew Alastair Cumming for editing this paper for the English revision.

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