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Lab 3

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MBK – Lab Report Name: __Jade Smart___

Section: ___________________

Aseptic Technique and Culturing Microbes

Part 3: Generating Microbial Cultures:

Observe your organisms after 24 hours to assess the growth patterns of all tubes.

If there is no observable growth allow the tubes to incubate an additional 24 hours. Record your observations.

Questions:

A. What is the difference between a bactericidal and bacteriostatic agent? Between sterilization and disinfecting?
The difference between the two is that bactericidal kills bacteria directly. While bacteriostatic stops the bacteria from growing. Bactericidal will injure the plasma membrane and the cell will leak out, killing it. Bacteriostatic stops bacteria from replicating.
The main difference between sterilization and disinfection is, that sterilization kills all microorganisms, while disinfection eliminates harmful microorganisms from inanimate objects and surfaces.
Sources:
http://study.com/academy/lesson/types-of-antibiotics-bacteriocidal-vsbacteriostatic-narrow-spectrum-vs-broad-spectrum.html http://www.diffen.com/difference/Disinfect_vs_Sterilize B. List five sterilization methods, how they work, and what they are used for.
The first form is steam. A machine called an autoclave is heated to 121-134 degrees Celsius. You hold the object there for 15 minutes for 121 degree Celsius or 3 minutes at 134 degree Celsius. It is used to inactivate all fungi, bacteria, viruses, and also bacterial spores. The second form is heating. This is performed by heating with flame, incineration, boiling of water, tantalization, or dry heat. These methods inactivate and kill microorganisms in objects like glass, metals. The third form is chemical sterilization, Chemicals like Ethylene oxide, Ozone[->0], Bleach, Glutaraldehyde and Formaldehyde, Phthalaldehyde, Hydrogen Peroxide,

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