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Lab Experience Analysis

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Walking into the first day of SEA-PHAGES, I was quite overwhelmed with what was going on. I have really never done any lab work whatsoever, so this was a new experience for me. Seeing so many new faces brought chills to my bones because I knew that I needed to find someone to collaborate with in order to succeed. I vividly remember the first day of aseptic training, where I kept asking a classmate besides me, Najia, on what to do. To this day, we still collaborate with each other. We often go over the Phage Discovery Guide to help us prepare for the next day of lab. If I had any questions, I would text her about it and she would do the same. We also consult with each other during the lab regarding out results, such as the presence of phage or contamination. Having an opening mind about making new friends to collaborate blossomed many other friendships with people throughout the lab. I think that …show more content…
Constantly using aseptic techniques and being mindful of being in the aseptic field was something that I had to get used to. I sometimes find myself moving away from the aseptic field and forgetting to flame a tube, which could contaminate the experiment. However, as I got more familiar with the lab techniques, the chance of this happening is now seldom. I also sometimes have trouble grasping the objectives of each experiment. This would require me reading the objectives over and over, and sometimes I would talk to my classmates about it. During week 4 and 5, this was during the rounds of purification, where I was trying to purify a phage. I completed many rounds of purification because I still had 2 different kinds of phages in my plaque assay. To make it worse, during round 4, I started seeing contamination happening. After talking to Dr. Moyer, we came to a conclusion that it was due to a staph bacterium. This frustrated me, but we are trying to move around it by hoping that filtering the lysate will remove the

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