Labset 2 Worksheep

In: Science

Submitted By rwilson30
Words 463
Pages 2
Labset Two Worksheet

1. What is differential staining? How does it differ from simple staining? (2)
Differential staining uses more than one chemical stain. It can help differentiate between different microorganisms, or different parts of the cell. The simple stain uses one stain and is used to see cell shape and size.




2. What are the differences between gram positive and gram negative cell walls? (2) A gram positive has a thick wall of peptidoglycan and stains purple. A gram negative has a membrane covering the peptidoglycan and doesn’t stain leaving it a pink color.






3. What is a mordant? What serves as a mordant in the gram stain protocol? (2)

A mordant is a chemical that can deepen the reaction of the dye. Iodine is the mordant in the gram stain protocol.




4. Why do gram negative cells stain pink? Gram positive cells purple? (2)

Gram positive cells have cell walls and can retain the dye. Gram negative cells do not have cell walls and cannot retain the dye. Cristal Violet is added to the sample and penetrates the cell walls, staining the cell in the gram positive. Gram negative will not retain color as there is no cell wall. A counterstain is added to make the cell visible and turns it a pink.



5. What is measured by the methyl red portion of the MR-VP test? (2)

It measures acid in a bacterial broth. It turns red in pH under 4.4, yellow in pH over 6.2, and orange in between.



6. What is measured by the Voges-Proskauer portion of the MR-VP test? (2)
It detects acetoin in a bacterial broth culture. A red is positive for acetoin and yellow-brown is negative.




7. What is methyl red? Why is it included in the MR-VP broth? (2)
Methyl red is a dark red powder used as a pH indicator. By adding the methyl red, the pH level can be known.




8. Define the following terms: (2)

Monotrichous:…...

Similar Documents

Labset 2 Worksheep

...Labset Two Worksheet 1. What is differential staining? How does it differ from simple staining? (2) Differential staining uses more than one chemical stain. It can help differentiate between different microorganisms, or different parts of the cell. The simple stain uses one stain and is used to see cell shape and size. 2. What are the differences between gram positive and gram negative cell walls? (2) A gram positive has a thick wall of peptidoglycan and stains purple. A gram negative has a membrane covering the peptidoglycan and doesn’t stain leaving it a pink color. 3. What is a mordant? What serves as a mordant in the gram stain protocol? (2) A mordant is a chemical that can deepen the reaction of the dye. Iodine is the mordant in the gram stain protocol. 4. Why do gram negative cells stain pink? Gram positive cells purple? (2) Gram positive cells have cell walls and can retain the dye. Gram negative cells do not have cell walls and cannot retain the dye. Cristal Violet is added to the sample and penetrates the cell walls, staining the cell in the gram positive. Gram negative will not retain color as there is no cell wall. A counterstain is added to make the cell visible and turns it a pink. 5. What is measured by the methyl red portion of the MR-VP test? (2) It measures acid in a bacterial broth. It turns red in pH under 4.4, yellow in pH over 6.2, and orange in between. 6. What is measured by the Voges...

Words: 463 - Pages: 2

Labset 2 Worksheep

...Dental Hygienist Program Colby Community College Associate Degree Checklist for a Complete Application Portfolio Read the following carefully in order to confirm that you have met the admission requirements and included all required documentation in your application portfolio. Include this checklist in your portfolio. _____1. I have requested three official high school transcripts. Sent to: CCC Dental Hygiene, 1255 South Range Ave., Colby, KS 67701 _____2. I have requested three official college transcripts from all colleges that I have attended. Sent to: CCC Dental Hygiene, 1255 South Range Ave., Colby, KS 67701. _____3. I have met the chemistry requirement (two semesters of high school or one semester of college chemistry with grades of “C” or better). _____4. I have completed all prerequisite courses. _____5. I have completed the admissions testing requirement, and I have included a copy of my test scores. Note: Applicants with an associate or bachelor’s degree are not required to test. _____6. I have completed the Transfer Credit Evaluation and Exchange Student Information forms. _____7. I have completed a letter of interest. _____8. I have completed the application for admittance for CCC and Admittance for NTC. I have attached $100.00 non-refundable application fee in the form of check or money order payable to Colby Community College, Dental Hygiene Department. _____9. I have included this checklist...

Words: 257 - Pages: 2

Jet 2 Task 2

... in this category should remain relatively similar to previous years. For example, Executive Compensation, Employment taxes, Payroll Service, Depreciation Expense, and Administrative Salaries were the exact same for Years 7 & 8. Given that these should remain the same in year 9; it is unclear why the company is budgeting more than $50,000 in this area than the previous year. I would suggest that the budgeted amount be somewhere between the year 7 & 8 amounts, given the positive prediction that sales will increase, which would put it between $1273867 and $1322075. This over statement in this category has also led to the company to under budget their Operating Income which they have budgeted at $80585. With the prediction of selling more units than year 8, the company should be budgeting a higher Operating Income than the previous year’s amount of $97,533. They definitely should not be indicating that they will be selling more but making less. This would be a bad financial sign for the company, as it is showing a lack of leadership and management skills. 2. No budget can be perfect, so keeping this in mind; one would expect variances to be present within the flexible budget. The variances found within this flexible budget, however, seem to highlight the flaws that were noticed and explained above. The first variance that is displayed is the unfavorable variance of $130,065.00 in Net Sales. This is due to the fact that the company sold 3423 units, not the...

Words: 1118 - Pages: 5

Unit 2 Assign 2

...Unit 2 Assignment 2: Microsoft Environment Analysis Vulnerabilities: * “Cross-site request forgery (CSRF) vulnerability in the Services module 6.x-3.x and 7.x-3.x before 7.x-3.4 for Drupal allows remote attackers to hijack the authentication of unspecified victims via unknown vectors”. * “SQL injection vulnerability in the Backend History module in TYPO3 4.5.x before 4.5.21, 4.6.x before 4.6.14, and 4.7.x before 4.7.6 allows remote authenticated backend users to execute arbitrary SQL commands via unspecified vectors”. * “The HP StoreOnce D2D backup system with software before 3.0.0 has a default password of badg3r5 for the HPSupport account, which allows remote attackers to obtain administrative access and delete data via an SSH session.” * “The default configuration of the Digital Alert Systems DASDEC EAS device before 2.0-2 and the Monroe Electronics R189 One-Net EAS device before 2.0-2 contains a known SSH private key, which makes it easier for remote attackers to obtain root access, and spoof alerts, via an SSH session.” * Use-after-free vulnerability in the mozilla::ResetDir function in Mozilla Firefox before 22.0, Firefox ESR 17.x before 17.0.7, Thunderbird before 17.0.7, and Thunderbird ESR 17.x before 17.0.7 allows remote attackers to execute arbitrary code or cause a denial of service (heap memory corruption) via unspecified vectors.” Elevation/Priority: * The priority level is medium, also should be seen to when possible. Priority Level...

Words: 565 - Pages: 3

Jet 2 Task 2

...Rachel Johnson Addy JET Task 2 March 29, 2014 JET Task 2 1. A budget is an itemized estimate of expected income as well as estimated expenses over a specified period of time. The reason to design a budget is to regulate spending. There are multiple causes of concern with Competition Bikes budget plan. The main cause for concern is that the sales estimated for year 9 is 3,510 units. This estimate is not supported by past sales data. Due to the current economic status, the amount of units sold from year 7 to 8 decreased 15% according to the horizontal analysis. Rider sponsorships were also reduced and will most likely remain limited in the future (WGU, 2014). There is some misleading and inaccurate information posted within the budget. Under the Selling, General, and Administrative breakdown “utilities” is listed at $150,000 on the vertical analysis for year 8 and year 9. Then there is another listing for “utilities and services” of $54,000. This is misleading because this entry was not listed in the expenses from the previous year. Another cause of concern is that the cash budget is not included in the uncollected receivables. CB makes expensive bikes so there should be room for uncollectable accounts. The uncollectable receivables budget could be used to cover the any uncollected payments. The reduction of sponsorships and the slow economy could possibly cause a rise in uncollectable accounts. There is also some concern because the operating income is...

Words: 1749 - Pages: 7

Unit 2 Asignment 2

...Unit 2 Assignment 1 Blank Answer Sheet Name: Date: Electronics ET2530 Chapter 2 (pp. 111-113) 2. A 1500 kHz carrier and a 2 kHz intelligence signal are combined in a non-linear device. List all the frequency components produced. - 1498, 1500, and 1520KHz 3.If a carrier is amplitude modulated what causes the sideband frequencies? - The non-linear mixing of the carrier and intelligence frequencies. 
 4.What determines the bandwidth of emission for an AM transmission? - It is twice the frequency of the highest audio frequency transmitted. The upper sideband is fc +FM where fc is the carrier frequency and FM is the modulation (audio) frequency .The lower sideband is fc – FM. The total bandwidth is 2*FM 5.Explain the difference between a sideband and a side frequency. - A sideband carries the modulation or "information". in old fashioned AM and FM transmission schemes, a carrier is broadcast in addition to the sideband(s). Only the sideband is needed to communicate. A spurious frequency that happens when a carrier is transmitted has no added "information" and just adds to the transmission bandwidth. Sometimes these occur by intermixing within the transmitter. a common example is CB splatter from overdrive of RF amps. 
 9.Draw a diagram (or indicate which text book Figure # shows this) of a carrier wave envelope when modulated by 50% by a sinusoidal wave. Indicate on the diagram the dimension from which the percentage of modulation is determined...

Words: 734 - Pages: 3

Chapter#2

...Chapter 2/Fundamental Economic Concepts Chapter 2 Fundamental Economic Concepts Solutions to Exercises 1. The number of checked bags should fall, as individuals internalize the external cost of added weight. However, the average weight should rise, as those checking bags would in all likelihood pack as much as possible to make up for the cost. 2. The wage in the market for oil spill cleanup efforts should rise, thanks to an increase in demand for labor. The wage in the market for similarly-skilled labor should also fall, as the relevant labor supply falls in response to the previous increase in wage. We would expect an increase in migration, as long as the wage of the home market was less than the wage that could be received in the Gulf region. 3. Projects A, C, G and B require a total of $875 million in investment. Project B, the lowest return project of this group is expected to generate an 18 percent rate of return. Ajax can raise up to $1,050 million, with the highest cost being 18 percent for the last $200 million. If Ajax were to choose any project beyond A, C, G, and Bsuch as project D (the next best project after B)that project would generate a marginal rate of return (16 percent), which is less than the marginal cost of funds needed to finance it, in this case 18 percent for the last $200 million up to a total of $1,050. Thus, the optimal capital budget is to invest in projects A, C, G, B, for a total of $875 million. 4. This question examines expected...

Words: 1045 - Pages: 5

Lab 2 Unit 2

...Lab 2.1 1. What are the Regulatory requirements? 2. What are medical rules or laws of private information that we need to know to set up this Database with the customer information (Data privacy)? 3. Please give me any specific requirements that are necessary? Business rules 1. Appointments 2. Social security 3. Medical record 4. Driver license 1. Middle initial 2. Are you a Jr. or Sr. Patient Entity | Patient Visit entity | 1. Medical record number | 1. Group number, appointment | 2. Social security number, Driver license | 2. Allergies | 3. Taking any medications | 4. Taking any medications | Composite key | Composite keys are the most common. | Make sure patient fills out all the whole application. Lab 2.2 Data Requirements 1. Allow Software Management Team and IT Staff to view, add, edit, and delete the types of software to be tracked. This includes type of software, licensing dates, status, and location. 2. Allow Software Management Team to enter, edit, and delete New Software Requests. 3. Allow Software Management Team to view the different licensing agreements and types. This includes viewing the pricing per agreement. 4. Allow Software Management Team to sign out software to users with administrative privileges. 5. Allow Software Management Team and IT Staff to update the status of software licenses. 6. Allow users, assigned IT users, Staff, and Software...

Words: 342 - Pages: 2

M5 2 Ra 2

...M5 Assignment 2: RA2: Course Project Task II (Etiology and Treatment) Etiology and Treatment This assignment follows up on your M3 A2 assignment in which you made primary and secondary diagnoses and also considered some of the differential diagnoses for your chosen case. Please do the following: - Modify your diagnoses (if needed) based upon the feedback I gave or the questions I asked you to spur your thinking; - Read five (5) peer-reviewed sources on the etiology of both your primary and secondary diagnosis. - Read five (5) peer-reviewed sources on the treatment of both your primary and secondary diagnosis. - Your paper should have these sections with the headings in bold: - - introduction o etiology of primary diagnosis o Etiology of secondary diagnosis o Therapeutic modality of primary diagnosis o Therapeutic modality of secondary diagnosis o Justification of the Selected Therapeutic Modality for the Primary Diagnosis o Justification of the Selected Therapeutic Modality for the Secondary Diagnosis o Application of the Treatment for the Primary Diagnosis o Application of the Treatment for the Secondary Diagnosis. o Citations / References in APA style. **Please focus on this, as this is still an issue. - The total page should be 8-10 pages. - NOTE: The developed emphasized that you should not discuss your differential diagnoses you did in Module 3 in this paper. These are......

Words: 290 - Pages: 2

Chapter 2

...FIN 612 Managerial Finance Week Two Assignment Your assignment for this week is to complete the following questions and problems from Chapter 2. Please submit your complete assignment in the course room by the due date. Chapter 2 Problems 2-1) An investor recently purchased a corporate bond that yields 9%. The investor is in the 36% combined federal and state tax bracket. What is the bond’s after-tax yield? 9% (1-T) = 9%(1-.36) =5.76% 2-2) Corporate bonds issued by Johnson Corporation currently yield 8%. Municipal bonds of equal risk currently yield 6%. At what tax rate would an investor be indifferent between these two bonds? 8% (1-T) + after tax corporate yield 6% - municipal tax exempt yield 8%(1-T) = 6% 8-8T+66 2=8t T=2/8 or 25% 2-7) The Talley Corporation had a taxable income of $365,000 from operations after all operating costs but before (1) interest charges of $50,000, (2) dividends received of $15,000, (3) dividends paid of $25,000, and (4) income taxes. What are the firm’s income tax liability and its after-tax income? What are the company’s marginal and average tax rates on taxable income? Income after operating costs: $365,000.00 Less: Interest Expense -$50,000.00 Plus: Taxable Dividends Received $4,500.00 Only 30% of the $15,000 in dividends are taxable. Taxable Income: $319,500.00 $319,500 falls into the $100,000 to $335,000 bracket so the corporate tax would be: $22,250 + .39 ($319,500 - $100,000) Tax Liability...

Words: 847 - Pages: 4

Unit 2 Assignment 2

...Asimo Unit 2 assignment 2 | AbstractASIMO is a humanoid Robot that was built to genuinely help people. Linda Vaughn | Asimo Unit 2 assignment 2 | AbstractASIMO is a humanoid Robot that was built to genuinely help people. Linda Vaughn | Linda Vaughn GS1145T 10/3/2015 Why Create ASIMO? ASIMO is humanoid robot created to duplicate human motion and genuinely help people. ASIMO took more than two decades of persistent study, research, trial and error before achieving a humanoid robot. ASIMO's design, development and operation rely on many different disciplines including Mathematics, Physics, Anatomy, Engineering and Computer Science. In 1986 Honda engineer’s set out to create a walking humanoid robot early models (E1, E2, and E3) focused on developing legs that could simulate the walk of a human. Models (E4, E5, and E6) focused on developing walk stabilization and climbing stairs. Then the head, arms and body were added to improve balance. Hondas first robot P1 was rather rugged standing at 6’2 and weighing at 386lbs. P2 had a more friendly design. P3 model was more compact standing at 5’2 and weighing 287lbs. ASIMO can run, walk on uneven slopes and surfaces turn smoothly and reach and grab for objects. ASIMO can also comprehend and respond to simple voice commands. It can also recognize the face of a selective group of individuals using camera eyes. It can also map environment and register stationary objects and can avoid moving objects as it moves...

Words: 598 - Pages: 3

Week 2

...University of Phoenix Material Week 2 Practice Worksheet Prepare a written response to the following questions. Chapter 3 For the following scores, find the mean, median, and the mode. Which would be the most appropriate measure for this data set? 2, 2, 0, 5,1, 4,1, 3, 0, 0, 1, 4, 4, 0,1, 4, 3, 4, 2, 1 Based on the scale of measurement for each variable listed below, which measure of central tendency is most appropriate for describing the data? The time (in years) it takes a sample of students to graduate college The blood type (e.g., Type A, B, AB, O) of a group of participants The rankings of college undergraduate academic programs Chapter 4 An expert reviews a sample of 10 scientific articles (n = 10) and records the following numbers of error in each article: 0, 4, 2, 8, 2, 3, 1, 0, 5, and 7. Compute the SS, the variance, and the standard deviation for this sample using the definitional and computational formulas. A psychologist interested in political behavior measured the square footage of the desks in the official office for four U.S. governors and of four chief executive officers (CEOs) of major U.S. corporations. The figures for the governors were 44, 36, 52, and 40 square feet. The figures for the CEOs were 32, 60, 48, 36 square feet. Figure the means and standard deviations for the governors and CEOs. Explain, to a person who has never had a course in statistics, what you have done. Note the ways in which the means and...

Words: 1096 - Pages: 5

Unit 2 Assignment 2

...Unit 2 assignment 2 Legislation- Human rights act- an act of parliament of the UK- the royal assent for this act was received the 9th of November 1998 with a commencement in 2000. It is an act to give further effect to rights and freedoms guaranteed under the European convention on human rights. Rights: -Right to life -Freedom from torture and inhuman or degrading treatment -Right to liberty and security -Freedom from slavery and forced labour -Right to a fair trial -No punishment without law -Respect for your private and family life, home and correspondence -Freedom of thought, belief and religion -Freedom of expression -Freedom of assembly and association -Right to marry and start a family -Protection from discrimination in respect of these rights and freedoms -Right to peaceful enjoyment of your property -Right to education -Right to participate in free elections This act promotes anti discriminatory practice in a way that it gives everyone the rights they deserve which can’t be taken away from anyone, therefore everyone is equal in what they can do. Data protection act- The data protection act defines UK laws on processing data on identifiable living people. It covers any data which can identify a person such as address, name, Humber, email, information is to be used fairly and lawfully. It is only used for limited specific purposes and in a way that is relevant, adequate and excessive. This promotes anti discriminatory practice as it helps protect...

Words: 4206 - Pages: 17

Level 2 Unit 2

... position then you will be work for certain day like 2 or three days or few hours in a week. * Temporary: - This means lasting for only a limited period of time; not permanent. For example if you are applying for temporary position then its means you are only working for limited period of time. DUTIES AND RESPOSIBILITIES: - A duty is something you must do by virtue of your position and is a legal or moral responsibility. For example, as Finance Officer you have to be responsible for the daily oversight of the finance system including checking, reconciliation and banking of monies received in Barnard castle school. Your duty will be to assist with monthly cash flow monitoring. OVERALL JOB OBJECTIVES: - This means what is the job role all about. For example, as a Finance Officer you have to be responsible for the smooth and efficient operation of the Finance Officer and the day to day management of the Finance Assistants. NOTES: - In notes the employer include necessary information that candidate should know while they apply for the job. What they should do when they have got the job. Some policy they need to know before they apply for the job. ADDITIONAL INFORMATION: - Extra information about job role. For example, when you apply for Finance Officer at Barnard castle school you have been asked to provide identification. Some important and necessary information the candidate need. Application Form POST TITLE: - In the application form you will be......

Words: 1595 - Pages: 7

Unit 2 Assignment 2

...Unit 2 Assign.2 Last 5 Published or Updated Security Advisories: Microsoft Security Advisory (2737111) Vulnerabilities in Microsoft Exchange and FAST Search Server 2010 for SharePoint Parsing Could Allow Remote Code Execution Published or Last Updated: Wednesday, July 25, 2012 Microsoft Security Advisory (2728973) Unauthorized Digital Certificates Could Allow Spoofing Published or Last Updated: Wednesday, July 11, 2012 Microsoft Security Advisory (2719662) Vulnerabilities in Gadgets Could Allow Remote Code Execution Published or Last Updated: Tuesday, July 10, 2012 Microsoft Security Advisory (2719615) Vulnerability in Microsoft XML Core Services Could Allow Remote Code Execution Published or Last Updated: Tuesday, July 10, 2012 Microsoft Security Advisory (2491888) Vulnerability in Microsoft Malware Protection Engine Could Allow Elevation of Privilege Tuesday, March 08, 2011 2. Do any vulnerability involve privilege elevation? Is this considered a high priority issue? Yes one, but its importance was not considered a high priority issue as asked 3. Identify and document at least three vulnerabilities and the solutions related to the client configurations. Advisory Number: 977981 Solution: This security update resolves four privately reported vulnerabilities and one publicly disclosed vulnerability in Internet Explorer. The vulnerabilities could allow remote code execution if a user views a specially crafted Web page using Internet Explorer...

Words: 337 - Pages: 2