Premium Essay

M and a Primer

In:

Submitted By studentexcel
Words 495
Pages 2
Finance Simulation: M&A in Wine Country Terminology Primer
Reservation Price: If you are a bidder, the highest price you would be willing to pay to acquire a target company is called your “reservation price”. It also is sometimes called a “walkaway price.” To calculate a reservation price you need to consider all available information about the target, your own company, and possibly other bidders and targets. Using all available information, and assuming the acquisition is completed, estimate the value of the target. If you pay more than this value the NPV of the deal will be negative; if you pay less it is positive. In this sense, the reservation price is the “zero-NPV,” or breakeven price. Conversely, if you are the target, your reservation price is the lowest price at which you would be willing to be acquired. Equity Consideration: Bel Vino and Starshine are considering a merger that would be consummated by an exchange of stock rather than a payment in cash. Such an exchange is called equity consideration, and such a transaction is called a stock deal (as compared to a cash deal). The following terms and considerations apply to stock deals.  Exchange ratio: Target shareholders are offered stock rather than cash in a stock deal. The offer is expressed in terms of an exchange ratio: the number of shares of the bidder’s stock offered in exchange for each share of the target. The Wine Country Simulation includes an Exchange Ratio Calculator to convert an offer price expressed in dollars to an equivalent exchange ratio based on each company’s then-current stock price. Announcement effect: An offer to acquire a target is immediately disclosed to the stock market (all three Wine Country companies are public companies). Accordingly, stock prices probably will change; such a change is called an announcement effect. It is important partly because it changes the value of a

Similar Documents

Free Essay

Primer Design Quizz

...PRIMER DESIGN QUIZZ Basing on the properties of primer for PCR process, a primers are designed to satisfy most of the requirements to be a good primer: The forward primer: 5’- VR THGAGTWYTA YRTYRTVG - 3’, the template from nucleotide 399 to 418 The revere primer: 3’- G TGAARTYVCG HACCSKN - 5’, the template from nucleotide 609 - 627 1) The reason for the region chosen: There are two basic reason for the region chosen for primer design: - Firstly, in this region, there are a high similarities of the DNA sequence of 4 organisms. - Secondly, the percentage of G and C are satisfied in the range of 40 - 60%. 2) The forward primer is VR THGAGTWYTA YRTYRTVG and have the direction of 5’ end to 3’ end: 5’- VR THGAGTWYTA YRTYRTVG - 3’ The reverse primer has an opposite direction which is 3’- G TGAARTYVCG HACCSKN - 5’ 3) The ideal length of PCR primer for general application should be between 18 to 24 bp (Dieffenbach, Lowe, & Dveksler, 1993). In reality, if the primer is too short, it will possess low specificity result in non-specific amplification. However, in another case that it is too long, it will decrease the template-binding efficiency at normal annealing temperature due to the high potential of forming secondary structure such as hairpins. The designed primer has the length of 20 bp with forward primer and 18 bp with reverse primer. This length is in the optimal condition for PCR primer. 4) The primers are always specified 5’ to 3’, and in the PCR...

Words: 882 - Pages: 4

Free Essay

Bangunan Air

...IRIGASI DAN BANGUNAN AIR “Bangunan-bangunan Irigasi” PROGRAM STUDI S-I TEKNIK SIPIL FAKULTAS TEKNIK UNIVERSITAS HALU OLEO KENDARI 2013 PENGERTIAN TENTANG IRIGASI Sejak ratusan tahun lalu atau bahkan ribuan tahun yang lalu , Kurnia ( 1996 ) menyatakan bahwa petani jawa barat telah mengenal jaringan irigasi sejak abad ke – 5 dan di jawa timur pada abad ke – 8. Indonesia yang memiliki iklim tropis atau yang terletak di iklim tropis basah dengan curah hujan yang tinggi pada beberapa bulan musim penghujan dan bulan – bulan kering pada kenyataannya masih sangat membutuhkan adanya sistem irigasi. Apabila disebutkan sistem irigasi maka orang cenderung hanya membayangkan suatu bangunan fisik bendung, dam ataupun saluran yang membawa air untuk mengairi padi atau sawah.Orang sering lupa bahwa bangunan tersebut dapat beroperasi dengan baik dan benar maka diperlukan juga Operasi dan Pemeliharaan yang baik dan benar. Menurut peraturan pemerintah No. 23 / 1998 tentang irigasi, bahwa Irigasi ialah usaha untuk penyedian dan pengaturan air untuk menunjang pertanian. Menurut PP No. 22 / 1998 irigasi juga termasuk dalam pengertian Drainase yaitu : mengatur air terlebih dari media tumbuh tanaman atau petak agar tidak mengganggu pertumbuhan maupun produksi tanaman. Sedangkan Small dan Svendsen ( menyebutkan bahwa irigasi ialah : tindakan intervasi manusia untuk mengubah aliran air dari sumbernya menurut ruang dan waktu serta mengolah sebagian...

Words: 1468 - Pages: 6

Premium Essay

No Files Was Uploaded

...agarose gel electrophoresis. NOTE: Read and all the instructions carefully before starting your experiment. Facilitators will guide you on the use of PCR machine and agarose gel electrophoresis. Be cautious when there is a need to handle items/equipments and the hazardous reagent, Ethidium Bromide (EB) in the EB room. Materials: Agarose gel electrophoresis set DNA ladder (marker) DNA loading dye DNA samples (labelled as DNA 1 and DNA 2; DNA 2 is obtained from P3) Deionised water Ice in tub Biohazard bin Disposable pipette tips Gloves Microcentrifuge (table top) Micropipettes (P1000, P100, P20) Microcentrifuge tubes (1.5ml) Paper Towels PCR machine PCR master mix (Taq Polymerase, MgCl2, dNTPs, buffer) Primers (labelled as Primer 1 and Primer 2) Methods: LAB 1: AM session (9.15am to 12noon) Part A: The Polymerase Chain Reaction (PCR) As a team, you will be using TWO DNA samples to perform TWO PCR reactions; DNA 1 will be given and DNA 2 which is obtained from your P3. NOTE: If you have TWO DNA samples from P3, choose the ONE with better purity or quantity. 1. Refer to Table 1 and complete the components and volumes needed to make PCR master mix. 2. For TWO PCR reactions, you need to prepare PCR master mix for 2.5 reactions (on ice). Pipette the solutions 1, 2, 3, 4 into a sterile microcentrifuge tube. What is the reason for preparing volumes for 2.5 reactions of PCR master mix solutions? To save time and minimize error in...

Words: 1931 - Pages: 8

Premium Essay

Cerebrospinal Fluid Infection

...International Journal of Biological Sciences Impact Factor ISSN 1449-2288 News feeds of IJBS published articles Manuscript Status/Login Contact 6 May 2015 Home Index & Ranking Current Issue Archive Cover Images Editorial Board Author Info Submission Special Issues Contact Journal of Genomics now in PubMed/PubMed Central. Submit manuscript... Top Introduction Materials and Methods Results Discussion Supplementary Material Acknowledgements References International Journal of Medical Sciences Journal of Cancer Theranostics Journal of Genomics PubMed Central Indexed in Journal Impact Factor PDF Int J Biol Sci 2015; 11(5):546-558. doi:10.7150/ijbs.11084 Research Paper Evidence for Fungal Infection in Cerebrospinal Fluid and Brain Tissue from Patients with Amyotrophic Lateral Sclerosis Ruth Alonso1, Diana Pisa1, Ana Isabel Marina1, Esperanza Morato1, Alberto Rábano2, Izaskun Rodal2, Luis Carrasco1 Corresponding address 1. Centro de Biología Molecular “Severo Ochoa”. c/Nicolás Cabrera, 1. Universidad Autónoma de Madrid. Cantoblanco. 28049 Madrid. Spain. 2. Department of Neuropathology and Tissue Bank, Unidad de Investigación Proyecto Alzheimer, Fundación CIEN, Instituto de Salud Carlos III, Madrid. Spain. How to cite this article: Alonso R, Pisa D, Marina AI, Morato E, Rábano A, Rodal I, Carrasco L. Evidence for Fungal Infection in Cerebrospinal Fluid...

Words: 9445 - Pages: 38

Free Essay

Finansijska I Aktuarska Matematika

...3. Anuiteti se menjaju po geometrijskoj progresiji 5.2.4. Anuiteti heterogeno (nepravilno) različiti ili proizvoljno odre eni 5.3. Konverzija zajmova 4 1. PROCENTNI I PROMILNI RAČUN Srazmerni račun pomoću koga direktan odnos dve veličine (tekuće i bazne, dela i celine) izražavamo tako što jednu od veličina (baznu, odnosno celinu) uzimamo kao 100 odnosno 1.000 jedinica nazivamo procentni odnosno promilni račun. Po imo do sledećih dogovora: 1% = 1/100 = 0,01; 6% = 6 ⋅ 1/100 = 6/100 = 0,06; o 1 /oo = 1/1.000 = 0,001; o 6 /oo = 6 ⋅ 1/1.000 = 6/1.000 = 0,006. Prema ovim dogovorima odnos broja 180 i 9.000 možemo prikazati ovako: 2 : 100 = 0,02 : 1 =  2%:100% 180 : 9.000 =  20 : 1.000 = 0,002 : 1 = 20 20‰:1.000‰ Uopštimo ovaj primer i napišimo sledeću proporciju: P : G = p : 1 ∼ G : P = 1 : p ∼ P = pG (1) G je oznaka za baznu velič inu, celinu ili tzv. čistu glavnicu; P je oznaka za tekuću velič inu, deo ili tzv. procentni (promilni) prinos; p je oznaka za tzv....

Words: 15506 - Pages: 63

Free Essay

The Effect of Biologically Active Vitamin D on Differentiating Skeletal Muscle Cells

...The effect of biologically active vitamin D on differentiating skeletal muscle cells Abstract Vitamin D deficiency can cause fat infiltration and this will lead to muscle 'quality' reduction. The probable mechanism of origin of these adipose cells is that abnormal trans-differentiation of myogenic precursor cells into adipocytes lead to the fat form within the intermuscular space. Myogenic precursor cells keep the potential to trans-differentiate towards the adipogenic lineage and VitD has potent effects on both adipogenesis and myogenesis. Therefore, the experiment is designed to investigate the effect of a broad range of concentrations of VitD3 active form (1,25(OH)2D3) on the capacity of the murine C2C12 muscle cell line to trans-differentiate towards the adipogenic lineages. C2C12 cells were cultured in adipogenic media and with increasing 1,25(OH)2D3 concentration (0, 10-13, 10-11, 10-9, 10-7 or 10-5M) for up to 6 days, the expression of muscle and fat gene markers were measured. The results showed that physiological concentration (10-13 and 10-11M) induces adipogenesis and myogenesis, while supraphysiological concentration (10-5M) inhibit both. (150) Introduction Nowadays, Vit D deficiency is considered as worldwide problem and influencing upwards of one billion people. Vit D deficiency can cause myopathy and atrophy of skeletal muscle (Holick 2006). Due to fat infiltration, there will be a concomitant reduction in muscle 'quality' (Ryall et al., 2008). The deficiency...

Words: 3124 - Pages: 13

Free Essay

FksdfñSd

...Contesta cada pregunta en el espacio asignado para cada una de ellas. No se corregir´n respuestas que a est´n en el espacio asignado a otras respuestas. e Las preguntas pueden ser contestadas con l´piz pasta o grafito. a El control tiene 60 puntos, por lo que la nota se calcular´ de la siguiente manera: a Nota = 1 + 6 × Puntaje 60 Preg. 1: de 15 Preg. 2: de 25 Preg. 3: de 20 Total: de 60 Nota: ECO301 Control 2 1. (15 puntos) Considera la siguiente funci´n f : R2 → R. o f (x, y) = xy + 1 1 + x y a) (5 puntos) Encuentra sus puntos cr´ ıticos, es decir, los que cumplen las condiciones de primer orden del problema de optimizaci´n no restringida. o b) (10 puntos) Determina si son m´ ınimos, m´ximos u otra cosa (puntos silla, puntos de inflexi´n) a o Respuesta: a) La condici´n de primer orden es ∇f (x, y) = (0, 0), por lo tanto: o ∂f =y− ∂x ∂f = x− ∂y 1 =0 x2 1 =0 y2 Para que estas derivadas parciales existan, se requiere que x = 0 y que y = 0. Esto conduce al siguiente sistema de ecuaciones: 1 1 y= 2 x= 2 x y Reemplazando el valor de y de la primera ecuaci´n en la segunda se tiene que: o x= 1 1 = x2 1/x2 x = x2 1=x En el ultimo paso se us´ el hecho de que x = 0. El valor de y se puede obtener como: ´ o y= 1 1 = 2 =1 x2 1 Por lo tanto, el unico punto cr´ ´ ıtico es (x, y) = (1, 1). b) La matriz hessiana de esta funci´n es: o Hf (x, y) = 2/x3 1 1 2/y 3 Si...

Words: 1469 - Pages: 6

Free Essay

Gene Marker Identification Targeting Toll-Like Receptor 4 (Tlr4), Breast Cancer 1 (Brca1), and Adenosine Triphosphatase 1 Alpha 1 (Atp1A1) Genes: Assessing Their Association with Subclinical Mastitis Cases in Dairy Water Buffaloes, Bubalus Bubalis

...Gene Marker Identification Targeting Toll-Like Receptor 4 (TLR4), Breast Cancer 1 (BRCA1), and Adenosine Triphosphatase 1 Alpha 1 (ATP1A1) Genes: Assessing Their Association With Subclinical Mastitis Cases in Dairy Water Buffaloes, Bubalus bubalis Thesis Proposal Cyndi Candelaria Biendima Patricia Malapit Cabatit Submitted to the Department of Biology College of Arts and Sciences University of the Philippines Manila Padre Faura, Ermita, Manila In partial fulfilment of the requirements for Undergraduate Thesis (BIO 200) TABLE OF CONTENTS Title Page1 Table of Contents2 Introduction3 Review of Related Literature6 Proposed Methodology14 Presentation of Results20 Literature Cited22 Line Item Budget26 Project Timeline27 1.0 INTRODUCTION 1.1 Background of the Study Cases of intramammary infections such as mastitis in water buffaloes contribute to large annual losses in milk production and net profit for smallholder farmers in the Philippines. Social and economic factors might prevent households from diagnosing, treating, and eliminating from circulation those animals or animal products, such as milk, that are afflicted with mastitis or which came from individuals afflicted with mastitis; this is especially true in the case of the asymptomatic subclinical mastitis, which tends to become chronic and difficult to eradicate by conventional antimicrobial therapies (Brouillette & Malouin, 2005; Ng et al., 2010). With the advent of technology...

Words: 7544 - Pages: 31

Premium Essay

LAMP Reaction Lab

...LAMP reaction The LAMP reaction was performed by using Loopamp DNA amplification kit, Eiken chemical CO., LTD., JAPAN. The 2X reaction mix composed of 40 mM Tris-HCl (pH 8.8), 20 mM MgSO4, 16 mM (NH4)2SO4, 0.2% Tween 20, 1.6 M Betaine and 2.8 mM dNTPs each. The LAMP reaction was optimized by incorporating different concentrations of primers and temperature. The LAMP reaction of total 25µl volume consisted of 2X reaction mixture (12.5µl), 2.0 μM FIP and BIP; 0.8 μM LF and LB; 0.2 μM F3 and B3 each, 1µl (8 units) of Bst DNA polymerase, Template DNA and PCR grade nuclease free water. Primer concentrations were optimized by using 0.8 μM or 1.2 μM or 1.6 μM or 2.0 μM of FIP and BIP primers each, 0.8 μM or 1 μM of LF and LB primers each, 0.2 μM...

Words: 714 - Pages: 3

Premium Essay

Cohort 1 Biochem

...labels for the diagram(s) and that you labeled the following: • DNA • replication fork • helicase- the helicase enzyme breaks up hydrogen bonds, which allows the DNA strand to unravel. • single-stranded binding proteins • leading strand • lagging strand • 5’ and 3’ ends of all DNA • primase- an enzyme that will create RNA Primer, creating a new strand able to bond to OH group. • RNA primer • DNA polymerase III- connects to one side of the exposed DNA chain reading from the 3’ to 5’ end. DNA polymarase III can only add from the 5’ to 3’ end of the newly developed strand of DNA. • Okazaki fragments (Wolfe, G, 2000). B. Insert your original diagram, or series of diagrams, with clear labels, that show the role of the ligase enzyme in the replication of DNA. Check to see that you described the function of the enzymes (enzyme names end in “-ase”) as part of the labels for the diagram(s) and that you labeled the following: • DNA • Okazaki fragments • DNA polymerase I- removes the leftover fragments of RNA primers and lays down the new DNA nuclotides....

Words: 692 - Pages: 3

Free Essay

Progeria

...Maheetha Bharadwaj Retrieved from the Progeria Research Foundation Website: progeriaresearchfoundation.org Table of Contents  Introduction and History  Research of Erikkson et al. (2003)  Causes: Mechanisms of mRNA Splicing  Truncated Lamina A: Progerin  Reverse Transcriptase Polymerase Chain Reaction  Effects  Treatment: Macro and Molecular  Conclusion  Bibiography Introduction and History  The Hutchinson-Gilford Progeria Syndrome (Progeria):      fatal disease that causes rapid aging Only 1 in 8 million have this disease First appearance: Hutchinson(1886) Second appearance: Gilford (1904) Only roughly 60 cases have been reported since: (Debrusk 1972, Brown et al. 1985 & 1986) Erikkson et al. 2003—f rst research done to show actual i causes and effects Research of Erikkson et. al (2003) 1. Out of 23 progeria, he found that 20 had a de novo mutation in LMNA gene(codes for nuclear Lamina A) 2. 18 out of 20: GGCGGT 1 out of 20: GGCAGC 1 out of 20: GAGAAG The causes of Progeria in the other three cases are unknown 3. Creation of splice site Mechanisms of mRNA Splicing  Introns vs. Exons  Donor acceptor pairs: GT-AG, GC-AG, AT-AC, and GT-GG. (Fong et al. 2006). The base pair progressions, GT, GC, and AT have the potential to set of a splice site.  The splice site signals the excision of genetic information, leading to a deletion of 150 bps, and 50 amino acids. Retrieved from: http://progeria2010researchproject...

Words: 1184 - Pages: 5

Free Essay

Essay

...Lenguaje y cerebro: conexiones entre neurolingüística y psicolingüística M. Jesús Paredes Duarte y Carmen Varo Varo Universidad de Cádiz El estudio de las relaciones entre lenguaje y cerebro constituye un objeto que atañe a muchas y diversas disciplinas. Representa, sin duda, uno de los capítulos más relevantes en el desarrollo histórico de la Psicolingüística y conforma el núcleo principal de los actuales contenidos de la Neurolingüística y su estricta implicación en el tratamiento de los trastornos del lenguaje. Otras muchas perspectivas vienen a crear una visión polimórfica de estas relaciones entre el lenguaje y el cerebro, despertando, no sin inconvenientes, pues a veces se solapan aspectos temáticos en este estudio, una consideración muy completa si tenemos en cuenta los diferentes puntos de vista. Una explicación de por qué el ser humano, frente a otros animales, posee “lenguaje” procede necesariamente del hecho de estar dotado de un cerebro distinto al de otras especies. Por otra parte, desde la perspectiva inversa, podríamos especular que el cerebro humano es diferente al de otros animales como resultado del empleo del lenguaje. Teniendo en cuenta la complementariedad entre estos enfoques, a la hora de tratar la relación entre cerebro y lenguaje, nos parece especialmente ineludible la conexión entre Psicolingüística y Neurolingüística. El presente trabajo surge de la necesidad docente de delimitar el objeto de estudio de dos disciplinas cuya conexión principal se...

Words: 5626 - Pages: 23

Premium Essay

Business Trip Result Report

...Taxation and Corporate Laws Updates on Indian Taxation and Corporate Laws knowledge by answering objective questions. Home Service Tax www.dateyvs.com/tax/customs-law-primer/customs-duty-basics-types-and-calculations/ 1/10 1/11/14 Indian Taxation and Corporate Laws » Customs Duty, basics, types and calculations Law Primer Tax Primer www.dateyvs.com/tax/customs-law-primer/customs-duty-basics-types-and-calculations/ 2/10 1/11/14 Indian Taxation and Corporate Laws » Customs Duty, basics, types and calculations Students Useful Sites For Corporate Laws For Taxation For Prime Institututions For Legal Information About Queries? www.dateyvs.com/tax/customs-law-primer/customs-duty-basics-types-and-calculations/ 3/10 1/11/14 Indian Taxation and Corporate Laws » Customs Duty, basics, types and calculations Customs Duty, basics, types and calculations Calculation of customs duty payable is as follows, w.e.f. 17-32012 Seq. Duty Description (A) Assessable Value Rs (B) Basic Customs Duty (C) Sub-Total for calculating CVD ‘(A+B)’ (D) CVD ‘C’ x excise duty rate (E) Sub-total for edu cess on customs ‘B+D (F) Edu Cess of Customs – 2% of ‘E’ (G) SAH Education Cess of Customs – 1% of ‘E’ (H) Sub-total for Spl CVD ‘C+D+F+G (I) Special CVD u/s 3(5) – 4% of ‘H’ (J) Total Duty (M) Total duty rounded to - Duty % Amount 10 12 2 1 4 Rs. 1,000 100.00 1,100.00 132.00 232.00 4.64 2.32 1,238.96 49.56 Total Duty 100.00 132.00 4.64 2.32 49.56 288.52 289 ...

Words: 1453 - Pages: 6

Free Essay

Paper

...Paper II: Biological Study 1. Abstract 2. Introduction In recent years, stable isotope records in speleothems (i.e., calcium carbonate deposits found in caves) have become more and more important as proxies of past climate variability (e.g., Spotl and Mangini (2002), Fleitmann et al. (2004), Harmon et al. (2004)). Speleothems, whiczare found in most continental areas provide high resolution records and can be precisely dated by U-series (Scholz and Hoffmann (2008)). The stable isotope signals of carbon δ13 C and δ18 O recorded in stalagmites are the most widely used proxy to reconstruct past climate changes. The isotopic composition of the drip water which feeds the stalagmites is influenced by several climate dependent processes occuring i) above the cave (e.g., geographical position, amount and type of vegetation, rainfall amount, temperature), ii) in the soil/karst zone (e.g., flow path of the solution, pCO2, host rock dissolution occuring under open or closed conditions iii) in the cave and on the surface of the speleothems (e.g., drip rate, pCO2). Inside the cave calcite precipitation results from the difference in pCO2, leading to a progressive degassing of CO2 from the drip water and hence, to an increase in supersaturation with respect to calcite. The isotopic composition of the precipitated calcite depends on the isotope value of the drip water and on isotope fractionation processes between the different species involved...

Words: 3824 - Pages: 16

Free Essay

Airbus by Wikipedia

...fuselaje. Posee una capacidad máxima de 853 pasajeros —en una hipotética configuración de alta densidad de clase turista—, convirtiéndose en el avión comercial más grande del mundo. Supera de esta manera al ya mencionado Boeing 747, al brindar un área útil de un 49% más que este último —según el propio fabricante—. Sólo es superado por el avión de carga Antonov An-225. Tiene una longitud de casi 73 metros y 24 metros de altura, estando su estructura formada en un 40% de fibra de carbono y otros modernos materiales metálicos. El primer vuelo de esta aeronave se llevó a cabo en Toulouse, Francia, el 27 de abril de 2005, realizando su primer vuelo comercial el 25 de octubre de 2007 con la aerolínea Singapore Airlines. Al disponer de una cubierta doble que se extiende a lo largo de todo el fuselaje, la superficie de la misma alcanza los 478,1 m², casi un 50% más que la de su principal competidor, el Boeing 747-400, el cual dispone de una superficie de cabina de 320,8 m². En una configuración clásica de tres clases —turista, negocios y primera— el A380 puede albergar entre 500 y 550 pasajeros. La versión de carga, pospuesta actualmente, disfrutaría de una capacidad de 150 toneladas y una autonomía de 10 400 kilómetros, sólo superada por el Antonov An-225. El A380, en su versión comercial, tiene un alcance de vuelo de 15 200 kilómetros, suficiente para cubrir rutas como por ejemplo: Madrid-Perth (Australia) sin escalas, con una velocidad de crucero de Mach 0,85 (900...

Words: 329 - Pages: 2