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In: Science

Submitted By kittychong

Words 892

Pages 4

Words 892

Pages 4

Group Member : Lim Phei Ching Lee Jer Win Chong Hui Li

Title :

Investigation of Action of Saliva and 3 M Hydrochloric Acid in Two Carbohydrate Solutions .

Objective :

To Investigate of Effect of Salive and Hydrochloric Acid in Carbohydrate Solutions .

Apparatus & Equipment :

Boiling tubes , Beaker , Graduated plastic dropper , Water bath, ~ 37 oC , Water bath, ~ 95oC

Materials :

Carbohydrate solution A , Carbohydrate solution B , Benedict‟s solution , 3 M Hydrochloric acid , 3 M Sodium hydroxide , Iodine solution

Procedures :

Part 1

1. Two boiling tubes containing 1 ml solution A and 1 ml of solution B were prepared respectively . 1ml of Benedict solution was added into each tube . Both tubes were heated together in the 95°C water bath for two minutes . Results were recorded in table 1 .

2. A few drops of solution A and B were added separately on a white tile . 1-2 drops of iodine solution were added on each solution .

Part 2

3. Boiling tubes were labeled 1,2,3,4 . 2 ml of solution B was pipette into each of four boiling tubes .

4. Tubes 1 and 2 were placed in a water bath of 37°C to heat up the solution .

5. Small beaker was salivated till it reached about 5 ml .

6. This step was done approximately at the same time . 2ml of saliva prepared was pipetted into tubes 1 and 4 . The contents of tubes were shaken well to ensure through mixing . 2ml of HCL was pipetted into tubes 2 and 3 .

7. Tubes 1,2,3,4 were incubated at their respective temperatures for 30 minutes .

8. More new boiling tubes were labeled 1' 2' 3' 4'.

9. 2ml of the contents from all these tubes were poured out into the respective newly labeled tubes ( eg 1 into 1' , 2 into 2' etc ) after 5 minutes of incubation of tubes 1 to 4 .

10. The acid in each tube labeled 2' and 3'...

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