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Methyl Red

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Lab 9 Methyl Red/Vog. Proskauer

A. What is meant by the term biochemical profile?
Biochemical profile is comprised of the specific pathway or molecules used in metabolic processes by an organism. This helps to identify a particular species of organism. B. What metabolic end product does the MR test for?
Methyl Red or MR is a test used to identify bacteria based on the way they metabolize glucose.

C. What does an orange color indicate as a result for an MR test?
The orange result in an MR test, is an indicator that the test was inconclusive. It is possible that the bacteria produces some acidic products, but not enough to overwhelm the phosphate buffer in the broth to turn it red.

D. What metabolic end product does the VP test for?
The end product we are testing for in the VP test is acetoin or acetyl methyl carbinol. I tested E.coli and S. epidermidis by preparing two tubes with incubated broth of each organism and added 12 drops of Barrit’s A Reagent to each tube. After mixing gently, I added 4 drops of Barrit’s Reagent B to each tube, shook gently and allowed to stand for 30 minutes. E. Why do you need to be careful not to jostle the VP tube while waiting for the results to show?
It is important not to move or shake the tubes because the Reagent A and B cause the acetoin to produce a maroon band that rises to the top of the broth indicating a positive VP result. If the tube was shaken it would stir the band into the broth and appear to be a negative result.

F. Which of the organisms, if any, fermented glucose?
E. coli and S.epidermidis both fermented glucose. The broth in both tubes turned red, indicating a pH at or below 4.4. G. Which of the organisms, if any, produced measurable acidic byproducts? E.coli and s. epidermidis both produced measurable acidic byproducts. H. What is the cellular role of catalase?
The role of

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