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Microbiology Lab-Bacterial Growth and Controlling It

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A. Describe the difference between an obligate and facultative aerobe.
Obligate aerobes are bacteria that require oxygen for growth. They will only grow at the top portion of the culture media where it meets the air and has a lot of oxygen. Facultative aerobes grow based on what is available to it. They prefer oxygen but oxygen is not necessary for growth. These aerobes mainly cluster at the top of the culture media where they have a high concentration of oxygen but will dispersed throughout because they do not require oxygen.

B. Discuss the temperature requirements of the organisms in this lab.
1. Explain what type of organisms they are.
Staphylococcus epidermis and Lactobacillus acidophilus are both a bacteria microbe. They grow best at normal body temperature, close to 370C. Microorganisms are classified according to their preferred temperature ranges. Because both grew at 370C, they would be classified as mesophiles. Mesophiles grow best in moderate temperatures ranging between 250C – 450C (Alonzo, n.d.).

C. Discuss under what conditions you would want to control the growth of microbes.
1. Explain what type of control you would employ in each situation.
To minimize the risk of contamination from unwanted organisms, aseptic technique is required. In this experiment I washed my hands with antibacterial soap. I then place on gloves to prevent contamination to the cultures from the bacteria on my skin and to protect myself from unintended exposure of unknown microbes. I also wore a mask to prevent microbes from my breath from contaminating the cultures and to protect myself. I used a disinfectant with 10% bleach solution to clean my work area. I used a tube of sterile medium, a sterile swab, and removed the cap without touching the inner surface or laying it down. Using a lit candle, I sterilized the top of the tube by running it through the

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