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Microbiology

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Task two A. Describe the difference between an obligate and facultative aerobe. Obligate aerobe refers to the organisms that cannot survive in environments without any presence of oxygen. Facultative aerobes refer to the organisms that can survive in the presence of oxygen, but they do not require it to survive (Gillespie, Hawkey, & Wiley, 2006). B. Discuss the temperature requirements of the organisms in this lab. The temperature requirement of the organisms in this lab is approximately 37℃. These organisms require this optimum temperature for enzyme reactions to take place. Too low temperature below the optimum deactivates enzymes reactions while too high temperatures above optimum denature enzymes. S. cerevisiae, s. epidermis and l. acidophilus are facultative anaerobe (Gillespie, Hawkey, & Wiley, 2006). C. Discuss under what conditions you would want to control the growth of microbes. To grow bacteria in a laboratory the conditions in the lab needs to mimic the particular microbes natural environmental. To culture microbe’s sterile, disinfecting, aseptic techniques need to be used. For instance, aseptic techniques are also needed to transfer food to the microbe. Sterilization is a process of destroying all forms of life on an object or in a material including endospores. Disinfectant are chemicals which kill growing microbes, but not necessarily spore and are used on inanimate objects. Antiseptic are chemical agent that prevents the growth microbes Sanitizers are chemical agents that reduce microbial populations to safe levels. To create a conducive environment for microbe’s temperature needs to be considered, most bacteria will grow between 30-40 degrees F there are some that will grow in 0 degrees F. An acidic or alkaline environment may be needed

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