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Nlc Lab Report

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5.1 Materials and Methods The following materials were used from the sources, without further purification. Diclofenac was procured as a gift sample from M/s Yarrow Chem Products, Wadala (E) and Mumbai. India. Cholesterol, Oleic acid, Triethanolamine (TEA), Acetone and Ethanol were obtained from Central Drug House Pvt. Ltd. New Delhi, India. All the other chemical were of the analytical grade. Double distilled water was used throughout the study.

5.2 Method For Preparation of Nanostructured Lipid Carriers (NLCs)
5.2.1 Plain nlcs (Without Drug) Melt emulsification and low temperature solidification method The formulations of different ingredients are shown in Table 1. Diclofenac and phospholipon 90G were dissolved in methanol and mixed …show more content…
5.3 OPTIMIZATION OF DRUG LOADED NLCs Preparation of Diclofenac NLCs involves various process variables out of which following were selected for the optimization of the formulation:
1. Effect of varying polymer concentration (Stearic acid).
2. Effect of varying drug concentration (Diclofenac).
3. Effect of varying stirring speed.

5.3.1 Effect of varying polymer concentration NLCs were prepared by method reported in section 5.2.1 by using varying stearic acid : Oleic acid concentration viz. 1:1, 2:1, 3:1 and 4:1 mg while other variables were kept constant. The effect of varying stearic acid concentration on the particle size and drug entrapment efficiency are reported in Table 5.1 and shown in Fig. 5.1 …show more content…
Analysis was performed at 25 ± 2oC. The NLC dispersion was diluted with double distilled water. Few drops of the dispersion were placed on the cover slip and allowed to dry in rotator drier to form a hard film. After formation, the images were captured (Fig. 5.4).

Fig. 5.4: SEM analysis of of NLC.

5.4.2 Determination of particle size, polydispersity index These measurements were performed in triplicates. The particle size and polydispersity index (PI) of nanostructured lipids were determined by Zetasizer Nano ZS (Malvern Instrument), Malvern, UK) at a temperature of 25±2oC at 90oC to the incident beam applying the principle of photon correlation spectroscopy. Dispersion were diluted with double distilled water to ensure that light scattering intensity, was within the instrument intensity range (Table

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