...A224 Recombinant DNA Technologies Problem 4 – Ask for More Protocol Background: DNA was isolated from the fluorescing bacteria. For subsequent cloning steps, additional copies of the gene responsible for the fluorescence are needed. Perform the Polymerase Chain Reaction (PCR) and analyze the PCR reactions via agarose gel electrophoresis. NOTE: Read and all the instructions carefully before starting your experiment. Facilitators will guide you on the use of PCR machine and agarose gel electrophoresis. Be cautious when there is a need to handle items/equipments and the hazardous reagent, Ethidium Bromide (EB) in the EB room. Materials: Agarose gel electrophoresis set DNA ladder (marker) DNA loading dye DNA samples (labelled as DNA 1 and DNA 2; DNA 2 is obtained from P3) Deionised water Ice in tub Biohazard bin Disposable pipette tips Gloves Microcentrifuge (table top) Micropipettes (P1000, P100, P20) Microcentrifuge tubes (1.5ml) Paper Towels PCR machine PCR master mix (Taq Polymerase, MgCl2, dNTPs, buffer) Primers (labelled as Primer 1 and Primer 2) Methods: LAB 1: AM session (9.15am to 12noon) Part A: The Polymerase Chain Reaction (PCR) As a team, you will be using TWO DNA samples to perform TWO PCR reactions; DNA 1 will be given and DNA 2 which is obtained from your P3. NOTE: If you have TWO DNA samples from P3, choose the ONE with better purity or quantity. 1. Refer to Table 1 and complete the components and volumes needed...
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...;Log apna banake chod dete hai. Rishta gairo se jod lete hai.. Hum to ek phool bhi na tod sake. Log to dil bhi tod dete ha Ek bus mein ladko aur ladkiyo ki team bani antakshari khelne ke liye. Girls: Hum tumko harakar dikhayenge.. Boys: Hum haar gaye, chalo ab dikhao Friendship SMS SMS > Clean > Friendship Page: 1 A single candle can illuminate an entire room. A true friend lights up an entire lifetime. Thanks for being the bright light of our friendship! 52 I believe in angels, the kind that heaven sends; I'm surrounded by angels but I call them my friends. Thanks for being my angel! 121 If you open my heart, guess what you're gonna see? It's you. As true friends are hard to find so I kept you. 137 A sweet friend is like a pillow. When you are tired, you sleep on it; When you are sad, you drop tears on it; When you are angry, you punch it; And when you are happy you hug it! Thanks for being my pillow! 332 I'm not popular, but I have nice friends; I'm not rich, but I have what I need; I may not be liked, but I know I'm loved. Thanks for being my very precious friend! For every day, I miss you. For every hour, I need you. For every minute, I feel you. For every second, I want you. Forever, I love you. 6 Ethics of Life: Before you pray-Believe Before you speak-Listen Before you spend-Earn Before you write-Think Before you Quit-Try & Before you Die-Live Always use these cosmetics in ur life. 4 ur Lips-truth; 4 ur voice-prayer; ...
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...Name of the group members Name: Farhana Akter Id# 1227142 Name: Fahima Akther Id# 1020938 Name: Afroza Akter Id# 1227050 Name of the organization COMFORT KIDS WEAR “Kids wear with love and care” Plan Outline Content Page * Executive Summary 1 * Company Description 2 * Product 3 * Market Analysis 4 * Strategy and Implementation 6 * Management Team 8 * Financial Analysis 9 * Risk Factor 11 * Conclusion 13 Executive Summery Comfort Kids Wear is a retail store for kids wear with good quality and latest trends owned by Fahima Akther, Farhana Akter and Afroza Akther as a partnership. It will be located at GEC in Chittagong city, Bangladesh. Total population of Chittagong is 40, 00,000. Our niche market is kids sector or people who buy kids wear. Our target audience is 5-15 age groups. The pricing strategy of our business is penetration...
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...Laboratory Report Style Guide A formal lab report is how you communicate the details of an experiment to the outside world. There are many ways of writing up a laboratory experiment. You have probably already done different forms in science and biology classes. The format we will use in this class is called “journal article format,” because it is the same format that scientific journals require for published articles. More practically, it is the format required by most colleges. It more or less resembles the format of an English term paper. Sections Summary A standard journal article laboratory report is organized into the following sections: Title: A single sentence fragment (no verb) that describes your experimental objective and gives some indication of the method (procedure). Abstract: A one-paragraph summary of the entire experiment—your procedure, results, and analysis. Introduction: A description of the scientific background for your experiment, including any previous experiments that your experiment builds on. (Remember to cite your sources!) The final sentence (analogous to the thesis statement in a term paper) is the objective of your experiment. Materials and Methods: A detailed description (in paragraph format) of the procedure for your experiment. Results: Your data, as you observed/recorded it. Note that this section is only for data that you observed or measured directly. Your analysis (including calculations) belongs in the Discussion section...
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...vacation to the northern areas last June. It was a well-planned trip with members of four families getting together for a chill-out. We congregated at Islamabad and started on our “voyage of discovery” early the next morning. To allow all of us to enjoy to the full we had hired a coaster driven by a very seasoned young driver who is familiar with the area. Our route took us from Islamabad to Abbotabad to Mansehra to Balakot to Kaghan to Naran to Shogran to Lalezar to Nathiagali to Ayubia to Islamabad & back to Lahore. The trip from Islamabad to Abbotabad was quite good. A good place for a short stopover in Abbotabad for refreshments & freshening up is Lahore Chatkhara Restaurant where the food & snacks are quite good. The tea is exceptionally good. No trip is complete without a puncture or two. Our coaster had one near the restaurant here. So we lost about half an hour in the process of changing the tyres and repairing the punctured one. Mansehra is a sleepy little town; Balakot was very neat and well arranged before the earthquake. The recently planted tea bushes above the road created a very pleasing sight. Kaghan is another place worth spending a day in. Little restaurants line the road and tracks meander down to the silvery Kunhar River rushing past the town. We had piping hot Pakoras and tea on our way back, here. The light drizzle made them even more enjoyable. The huge walnut trees looked majestic and their fruit was in the middle of its growth cycle. My son had...
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...Generic Benchmarking Worksheet Task A: Problem/Opportunity Statement Instructions for Task A: In the Response row, write out the problem/opportunity statements for the scenario for each of the team members. Response to Task A: Generic Benchmarking—The purpose of generic benchmarking is to identify potential solutions to the problem statements defined in Task A. You will do this by looking at how companies in other industries have dealt with similar issues. Task B1: Generic Benchmarking: Topics Instructions for Task B1: In the Response row, identify the topics for which you need information in order to identify potential solutions to the problems identified in Task A. • In the Response row, list three to five topics that you will research in peer-reviewed journals, on Web sites, and in popular publications such as magazines and newspapers. • In the Response row, provide a justification for each topic. Response to Task B1: Task B2: Generic Benchmarking: Companies Instructions for Task B2: In the Response row, identify companies that have faced and addressed similar situations (successfully and unsuccessfully). • In the Response row, list two to three companies for each topic identified in Task B1. • In the Response row, identify those that have been successful and those that have been unsuccessful. • In the Response row, summarize your key findings for each company as they relate to the scenario. Response to Task B2: Task B3: Generic Benchmarking: References ...
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...Article 2 Day: Class: 9 c Introduction Having acquainted myself with certain parameters by now I was on a lookout for some other equally valuable analysis. During the 2 weeks PSE 1 we are required to keenly observe and adopt the best practices/methods of teaching in the teaching profession. In order to facilitate this we become parts of a real classroom teaching process and frame our views on the basis of what we observe. Purpose of the study In order to further upgrade my understanding of the teaching profession I chose to observe this class based on home task, the teachers personality and the strength of the lesson. Herein this piece of writing I would therefore explore the highlights and let downs of these parameters, evaluate them and form an analysis for future reference. I would stress on the distinguished factors of the study in the reflections I make and share my inputs on the same. Similarly I would identify the drawbacks and asses the reasons why they occurred and what could have been done to prevent the same. Once again I got the opportunity to asses and evaluate a class being taught the same lesson as observed by me in the previous class. This gave me a golden opportunity to distinguish between two methods of teaching the same lesson. It is a fact, that the parameters analyzed differ but the stark contrast in the teaching style of two teachers intrigues me to assess the strength of the lesson. Observations Social Science in view of most of the...
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...decision from free will was compromised by rules. I was in DCFS my whole life along with my younger sister and brother. At the time we were placed with an older woman around 50-54 years old. I had gotten into a physical altercation with her to protect my little brother. I was raised to respect, honor, and obey my elders and any adults but she had pushed the line too far. She was from the south and was raised the strict southern way. When certain things occurred and things went wrong it always resulted in one of us getting a bad whooping. By state laws and regulations I knew as a ward of the state. I could always pick up the phone and report any abuse or neglect to the abuse hotline or my caseworker. On this day I came home from basketball practice and I was starving. The first thing I noticed when I got in was Paul was very upset crying. I didn’t know what happened but I knew my brother and I wanted to know why he was so distraught. I asked him what was wrong and he said he had been sent to his room when he got home from school for getting in a fight at school. that was 5 hours ago and he said he didn’t eat because she was told to stay in his room. I didn’t think that was an ethical decision to make so I told him It would be ok and that he needed to get himself together fast. I asked my foster mom what was for dinner and she said fish spaghetti and garlic bread so I said ok I'll get washed up for dinner. I asked Jazmine if she was ready to eat and if she had...
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...SWOT Analysis is to figure out strengths, weaknesses, opportunities, and threats of companies. This document explains about the ABC Company SWOT Analysis. The SWOT Analysis is helpful to know that ABC's potential areas of improvement and also what are all the risks. One of the strengths for ABC Company is customer loyalty and have a solid base and background in the restaurant market. Since ABC company is an established company, the risk on launching new products is less and the product information is easily accessible. ABC company has a good growing market. Weaknesses for ABC company are heavy loss in sales because of expired food and the cost of items are very expensive compared to other restaurants. The billing system in that restaurant is not great and effective. The potential opportunities for ABC company would be they should reduct the cost of some of their items and get people attention by inccreasing customer relations, focussing on efficiency and moving to a main part of the town. Through local advertisements also they can get public attention. There are lot of local restaurants are in that town and price wise they are very competitive. They are the big threats for ABC company. The only way ABC can compete them is by reducing price and increasing quality of food. Upon breaking into the Mexican retaurant busines market ABC need to prove the quality of their food is best and rate wise they are very competitive with others. Approximately there are...
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...------------------------------------------------- Как отличить настоящий мед от фальшивого Мед издавна славится своими полезными свойствами. Но сейчас важно не прогадать и приобрести действительно настоящий натуральный мед, иначе пользы от него не будет. Как проверить качество меда 1. Для того чтобы определить зрелость жидкого (незасахарившегося, свежего) меда, в него опускают ложку и начинают вращать его. Незрелый мед стекает с ложки, а зрелый – наматывается, ложась на ложку складками, как лента. 2. Возьмите жидкий (незасахарившийся) мед на пробу, опустив в емкость тонкую палочку. Если это настоящий мед, то он тянется вслед за палочкой длинной непрерывной нитью, а когда эта нить прервется, то она целиком опустится, образуя на поверхности меда башенку, пагоду, которая затем медленно разойдется. Фальшивый же мед поведет себя, как клей: будет обильно стекать и капать с палочки вниз, образуя брызги. 3. Kачественный мед не должен пенится. Пенистость свидетельствует о брожении, т.е. порче меда. Натуральный мед не может бродить, т.к. он бактерициден. 4. Со временем мед мутнеет и густеет (засахаривается) – это верный признак хорошего качества. Жидкий мед бывает, как правило, летом (июль-август) в период его откачки. Максимум через 1-2 месяца (в зависимости от сорта) он кристаллизуется. Поэтому, если зимой или весной продается жидкий мед, значит, он или подогрет, или фальсифицирован. Следует помнить, что при нагревании до температуры +40°С и выше мед утрачивает свои основные...
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...Child Care Center Risk Reduction Plan Program Name: _____________________________________________ License #: _____________ Child care centers must develop a risk reduction plan that identifies the general risks to children served by the child care center. The license holder must establish specific policies and procedures or refer to existing policies and procedures that minimize identified risks, train staff on the procedures, and annually review the procedures. [Minnesota Statutes, section 245A.66, subdivision 2] 1. Physical Plant. Identify specific risks to children based on an assessment of the physical plant where licensed services are provided. The assessment must include an evaluation of the required factors listed below in column 1. Write each identified risk in column 2; insert additional lines as needed. In column 3, write specific policies and procedures you have developed and implemented to minimize each risk, or in column 4, reference existing policies and procedures that minimize each risk. Physical plant Identified risks Policies and procedures Existing policies and factors developed and implemented to procedures that minimize required to be minimize the risks the risks assessed Condition and design of the facility Condition and design of the outdoor space Bathrooms Page 1 Minnesota Department of Human Services Division of Licensing Office of Inspector General November 2012 Physical plant factors required to be assessed Storage areas Identified...
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...用图像记忆法来记忆英语单词，关键在于把抽象的字母转化为熟悉的图像，然后把这些图像组合起来进行联想。这样，原本抽象的单词就能变成一个生动的画面，就能像看电视、看电影那样来进行记忆，不仅记得快、记得牢，而且记忆的过程充满着乐趣，会让你从此爱上背单词！ 经过我们深入系统的研究，单词记忆的方法，可以归纳为最简单的四个步骤： 第一步：找单词（包括找完整单词、近似单词、词根词缀）； 第二步：找拼音（包括找全拼、近似拼音、拼音首字母）； 第三步：找编码（找字母编码）； 第四步：找谐音（包括整体谐音和部分谐音）。 这四个步骤，简化一下，就是“单凭编写”，也就是说，要记忆单词，单凭编写就可以获得非常好的效果。 WWW.PAPERLW.NET 下面，我们就来详细介绍“四步背单词”的四个步骤。 中国论文网-英文版 中国论文网海外版-www.paperlw.net 第一步：找单词： 中国论文网-英文版 www.paperlw.net 一，找完整单词： 1，hesitate――犹豫 分析：he――他；sit――坐；ate――吃（eat）的过去式。 记忆：他犹豫地坐着吃。 2，catcall――喝倒彩 分析：cat――猫；call――喊叫。 记忆：猫对着你喊叫，就是在向你喝倒彩。 3，hatred――仇恨 分析：hat――帽子；red――红色的。 记忆：小红帽非常仇恨大灰狼。 4，forget――忘记 分析：for――为了；get――得到。 记忆：为了要得到新的，需要先忘记旧的。 5，butterfly－－蝴蝶 分析：butter――黄油；fly――飞。 记忆：蝴蝶很喜欢在黄油上面飞。 6，excerpt n. 摘录，节录 分析：except——除了……之外；r——像小草。 记忆：除了关于小草的部分之外，其它我都摘录完了。 7，freight n. 货运，货物 字串2 分析：fr——“飞人”的拼音首字母；eight——八。 记忆：飞人运走了八件货物。 8，justice n. 正义，合理性，法官 分析：just——刚才；ice——冰。 记忆：法官刚才加了一块冰。 9，leaflet n. 传单，小册子 v. 散发传单 分析：leaf——树叶；let——让。 记忆：这些树叶都让我当作传单发出去了。 10，menace n. v. 威胁 分析：men——男人们；a——“一”；ce——“厕”的拼音。 记忆：男人们在一个厕所里受到了威胁。 11，glove――手套 分析：g――“哥”的拼音首字母；love――喜爱。 记忆：哥哥很喜爱这双手套。 12，smother――窒息 分析：s――外形像蛇；mother――妈妈。 记忆：一条蛇把妈妈缠得快要窒息了。 中国论文网-英文版 二，找近似单词： 1，widow――寡妇 分析：window――窗户；n――“泥”的拼音首字母。 记忆：寡妇的工作是要把窗户上的泥擦干净。 2，2，knack——诀窍 分析：knock——敲门；a——“一”。 记忆：敲门的诀窍是只敲一次。 3，peak――山顶 分析：speak――说话；s――外形像蛇。 记忆：有一条蛇在山顶上说话。 4，lurch——突然倾斜分析：lunch——午餐；r——“人”的拼音首字母。 记忆：那个人正在吃午餐的时候，桌子突然倾斜了。 5，roof――屋顶 分析：room――房间；f――外形像拐杖。 记忆：我房间的屋顶上插满了拐杖。 ...
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...Field Training Officer 4/10/12 Field Training Officer Explain the reasons why an effective FTO should be patient and pro department. A FTO should be patient because he or she is dealing with new trainee for a predetermined amount of time. The burden of training new recruits, and instilling the values of the department in them falls on the field training officer. The first few days are the most critical time of the new trainee steps to becoming a good officer. During this time the stress level of a new trainee is at an all time high and mistakes will be made and as the FTO you must remain calm. A good FTO is pro department he or she must let the new trainee know how Bell county sheriff department works and where the job can take you in the future. Why would teaching trainee short cuts be a bad idea? A new trainee is like a new born he or she has now idea about the policy and procedure so teaching a trainee short cuts is put lives in danger, it goes against providing safe secure facility. It also puts other officer that may work with the trainee in the future in harm’s way. A short cut always leads to mistake and mistake in the sheriff’s department is unexcitable. Short cuts are unethical and irresponsible. Short cuts go against the departments mission statement, will not allow you to enforce laws, are protect life and property. Explain an effective field Training Officer, what qualities they must have. The qualities that a Field Training Officer should have that...
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...the auditor significant difficulty in applying auditing procedures he or she considers necessary. Other findings that could result in modification of the auditor’s report. Compliance of Auditing Standards and Audit Documentation – a must for all practice units satisfy any applicable legal or regulatory requirements for records retention. Internationally the norm is assemble the final audit file and submit it for archiving within 60 days from the date of our audit report. The PCAOB standard has shortened this period to 45 days. 51 The auditor would also need to adopt procedures that enable him or her to gain access to the documentation throughout that period. One way for auditors to accomplish this is by creating a policy to maintain electronic documentation. One needs to bear in mind that archiving needs to be done in a format that ensures its compatibility with newer versions of audit software or by retaining older versions of such software and, if necessary, the hardware on which it runs. Also, working papers for a specific audit engagement are assembled in a separate audit file. • • Conclusion 52 Auditors in India will have to gear themselves up to meet the new...
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