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Nt1310 Unit 2 Lab 2

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Lab 2 The purpose of Lab 2 was to examine the role and nature of restriction enzymes as well to take the first steps in producing a recombinant DNA molecule. Plasmids are circular DNA pieces found in bacterial cells. Plasmids are used in genetic engineering to expedite gene cloning and gene expression (through protein production) in bacteria. Through cloning, antibiotic resistance genes have been placed inside of plasmids and the antibiotic resistance genes function as selectable markers – genes that allow scientists to select between bacteria that do and do not have the plasmid. If a bacterium does in fact possess a plasmid that has an antibiotic resistance gene then that particular bacterium will be able to reproduce in an environment with that specific antibiotic present. Bacteria that do not possess a plasmid with an antibiotic resistance gene will not be able to grow. Therefore, antibiotics can be used to pin point bacteria that are resistant and presumably contain a plasmid with the resistance gene from those that are not resistant …show more content…
The plasmids formed will represent recombinant DNA because the four restriction fragments have been recombined in new ways. There are many ways the fragments could have combined, producing many kinds of recombinant molecules. When the enzymes cut the plasmids at the BamH I and Hind III restriction sites, the cutting left “sticky ends” on the two fragments. The sticky ends on any cut DNA can be bonded (ligated) to any other DNA fragment with complementary sticky ends. Since pARA has two enzyme restriction sites, the digestion will result in two fragments (one that is 4018 bp and another that is 40 bp). One of the fragments carries ampr gene and the other fragment does not carry any genes. The cutting of pKAN-R will result in two fragments (one that is 4706 bp and another that is 702

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