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Nt1310 Unit 4 Lab Report

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Cesium iodide and Alkyl metals salt clusters (Na, K, Rb…. Acetates,), with wide m/e range are commonly used for calibration of mass spectrometers. Polymers as poly ethylene oxide or polypropylene oxide are other excellent calibrators. Dish washing compound lauryl sulfate ethoxylorte, is a good calibrator for the compound for negative ion ESI.
Water cluster is another good reference standard, with closely spaced reference peaks the other reference compound used are cesium salt of mono butyl phthalate, perfluyorinated acids, aminomethylphosphonic acid etc. In general a compound is used as a reference, depending upon the calibration required in the lower or higher scale of spectrometer and a with the stable molecular ion with exact m/e value. …show more content…
Mass of C12 is taken as 12, is a nominal mass and is a whole number. 1/12 of carbon C12 is 1.nominal mass or µ (a.m.u).
In the spectrum it is nominal mass of a sample which is recorded against their relative abundances. Nominal mass for CO2 is 44 and for CH3OH is 32. It is an integer, and is sum of integer masses of elements present in sample compound.
Isotopic mass is the exact mass of an isotope of an element, and it is 12 for C12, it is only integer mass. For all other isotopic atoms mass is determined with reference to C12, 1/12 of carbon is µ, is equal to 1.66055x10-27 kg, is taken as standard mass since 1961.
Isotopic mass is the mass of that particular atom of an element with reference to C12, 12.000000, For C13; it is 13.000355 for hydrogen, (_1^1)H, 1.007825, and for (_2^1)H 2.014101 µ.
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