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Paternity Testing

In: Science

Submitted By somrjones
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Student number: 3003481…………………

DNA Paternity testing

Two rams at the Easton College farm, a Norfolk Horn sheep and a Southdown sheep escaped from their field and ran in the ewes’ enclosure. The ewes were all Suffolk breed, an old native British breed with excellent lamb growth rate. You were asked to help with identifying the father of the lambs and you decided to try a DNA paternity test with two lambs. http://www.norfolkhornbreeders.co.uk/history.php

I Protocol
To perform a quick screen using agarose gel electrophoresis, you collected some cells from the lambs and the potential parents in order to extract and process the DNA ready for electrophoresis.
You labeled your tubes as followed:

Sample A: Ladder
Sample B: Suffolk ewe
Sample C: Lamb 1
Sample D: Lamb 2
Sample E: Norfolk Horn ram
Sample F: Southdown ram

II DNA electrophoresis procedure
Read the procedure carefully before you start. well | 1 | 2 | 3 | 4 | 5 | 6 | Sample | A | B | C | D | E | F |

Use a plastic pipette to pour some buffer near the comb.

GENTLY remove the comb and drop some more buffer in the wells.

Using a micropipette, load 20l of each of the samples into separate wells in the agarose gel as indicated in the above table. Do not stab the pipette tip into the wells and pierce the bottom of the gel!

When all samples have been loaded onto the gels, place them into the tank. Call a demonstrator to assist you with covering the gels with the buffer.

Cover the tank with the lead and connect the electrodes to the electrophoresis power pack the correct way! DNA will migrate towards the positive electrode, which is usually coloured red.

Press the RUN button. The gels will be run at 150 volts for about 30 minutes.

TURN OFF THE ELECTROPHORESIS POWER PACK. Switch off at...

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