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Pectinase Lab

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Introduction Through this lab, we are trying to find out if 6 drops of the enzyme pectinase, 6 drops of the enzyme cellulose, 3 drops of each enzyme, or 6 drops of distilled water with be most effective in creating apple juice when mixed with 50 grams of apple sauce and then filtered. With that, we need to figure out which will be most cost effective so that the apple juice company knows which substance will benefit them the most. We are aware that pectinase costs $50 per liter and Cellulase costs $100 per liter, which will make an immense impact on the decision, taking data into consideration, of course. When conducting this experiment, our independent variable was substance type. Our different types of substances were pectinase, cellulase, …show more content…
A juice company would be interested in using pectinase to produce juice from applesauce because during a chemical reaction, the bonds break, changing the applesauce into apple juice.

Procedure
Collect materials: applesauce, pectinase enzyme, cellulase enzyme, funnels, filter paper, lab aprons, splash-proof goggles, paper towels (for cleanup), droppers, stirring rods, graduated cylinder, tap water, cups, watch/clock, balance, pen.
Put on safety goggles and a lab apron.
Label 4 cups with the trial number and the substance
Measure 50 grams of applesauce into a cup with a balance.
Put 6 drops of cellulase in one cup.
Stir each cup, using different stirrers per substance, 30 complete stirs in a clockwise direction.
Let the applesauce and enzyme sit for 3 minutes.
Filter: -Place the funnel on top of a 100 mL graduated cylinder -Place the filter into the funnel -Gently poor the mixture into the graduated cylinder system, using a spoon, if necessary, to get out all of the contents -Let it sit for three minutes Measure the amount of juice in the graduated cylinder in mL and record in your data table
Repeat steps 5-8 for 6 drops of pectinase, 3 mL of pectinase with 3 mL of cellulase, and 6 mL of distilled …show more content…
While I believed that 3 drops of cellulase mixed with 3 drops of pectinase would be the most efficient in the creation of apple juice because they work on different substrates. The results came out that pectinase is the best option to create apple juice, with the highest average at 10.67 mL of apple juice. Cellulase proved to be the least efficient with only 7.34 mL of apple juice. However, the positive to the results on pectinase is that is is half the price of cellulase. This will be beneficial to the apple juice production company so that they can get more for

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