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Pepper Seed Dna Extraction

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Pepper Seed DNA Extraction
Biochem lab: CHE 452L marisol gomez
Pepper Seed DNA Extraction
Biochem lab: CHE 452L marisol gomez
2015
2015

INTRODUCTION
The jalapeno is a member of the capsicum family, along with many other peppers. The usual methods for characterization of different pepper species are based on their morphological and physiological traits, however this many not always be enough. For peppers, their traits are influenced by things like their genotype or their specific environment. Genomic markers can allow for a more direct comparison of closely related individuals (Ansari and Khan, 2012). In our case we focus on DNA extraction. The two basic parts of a DNA extraction procedure include the breaking of the cell walls to expose the DNA and the use of enzymes to remove contaminants. The DNA is analyzed for purity by taking the absorbance. The pure DNA is then visualized by gel electrophoresis. The DNA extraction of plant seeds is difficult because of their cell wall. The method used to break the cell wall includes grinding the seeds with liquid nitrogen. The addition of DNAzol is used to isolate genomic DNA (Chomczynski et al. 1997). Restriction enzymes are necessary to fragment patterns of the DNA and in turn making it easier to analyze the DNA through gel electrophoresis.
BACKGROUND
The purpose of our experiment is to extract the DNA from pepper seeds to be able to compare and contrast the similarities in their DNA. The extraction of DNA from a plant is slightly more difficult than that of mammals. This is due to the presence of a rigid cell wall. To overcome this problem, the pepper seeds are grinded using liquid nitrogen (Manen, Jean-François, 2005). The liquid nitrogen facilitates the pulverization of the seeds by flash freezing them and causes the outer shell to become fragile. Plant DNAzol is a guanidine thiocyanate and detergent

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