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Regulation of Adipocytes

In: Science

Submitted By jgindt
Words 287
Pages 2
Seminar
-Relationship between vitamin D and obesity
-Obesity…complications, psychological, financial
-Functions of Adipose tissue -regulation of insulin, blood pressure
-changes in adipose tissue during obesity- obese with metabolic dysfunction( increase inflammation)
-Vitamin D metabolism
-Vitamin D status is inversely correlated with markers of adiposity
-Vitamin D regulates adipose tissue function
-Adipocyte differentiation- pre-adipocyte- early adipocyte - mature adipocyte -glucocorticoid and insulin promote adipocyte differentiation.
Does 1,25(OH)2D Inhibits 3t3-l1 differentiation in a dose-dependent manner
-Lipid storage in adipocytes(image)
-1,25(OH)2D stimulates lipolysis in 3T3-L1 Adipocytes
-TAG storage in adipocytes
-effect of 1,25(OH)2D on lipolytic protein expression.
Conclusion:
1,25(OH)2D stimulates:
-increased PKA-dependent lipolysis
-increased HSL phosphorylation
-increased CGI-58 protein expression
-decreased ATGL protein expression
Extracellular fatty acid accumulation does not account for reduction in TAG stores.
1,25(OH)2D stimulates fatty acid uptake in 3T3-L1 Adipocytes
1,25(OH)2D reduces acetate incorporation into fatty acids
1,25(OH)2D stimulates:
-decreased glucose incorporation into fatty acids
-decreased ACC1/FAS activity
Effect of 1,25(OH)2D on 3T3-L1 Adipocytes:
Increased fatty acid turnover
-decreased glucose incorporation into fatty acids
Implications:
-Reduced TAG accumulation may alleviate chronic inflammation associated with expansion of adipose tissue.
- Inhibition of de novo lipogenesis in adipose tissue, coupled with increased insulin sensitivity in skeletal muscle, may be metabolically favorable.
Future Studies:
-Identify the lowest concentration of 1,25(OH)2D that is sufficient to induce these changes in 3T3-L1 adipocytes.
- Determine whether 1,25(OH)2D- induced regulation of lipid metabolism specific to murine adipocytes.
-Identify vitamin D target genes responsible for 1,25(OH)2D- induced changes in adipocyte lipid metabolism

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