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Research Article Critique

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RESEARCH ARTICLE CRITIQUE

Biomedical Science

Brief summary

The article selected for this paper is “A study into the potential role of Survivin localisation in resistance to drug-induced apoptosis” written by Helen Angell. The paper is about the role of an anti-apoptotic factor from the class of caspases. The subcellular localisation of surviving can play important role in the therapy of breast cancer. Cancer is an abnormal process in which the cellular apoptosis is inhibited. Survivin is a protein known to regulate cell division and apoptosis. Chemotherapy in cancer patients in targeted to induce apoptosis. Surviving has been known to be localised in the cytoplasm and nucleus of the cells. The main therapeutic role of Survivin comes from the fact that the sensitivity of the breast cancer cells to chemotherapy is increased in its presence within the nucleus.

Description of the methods and techniques used

Various methods have been used by the author. All lab-based methods are used for the determination of localisation and presence of the protein in the cell.

• The fourth method used is tissue culture, for the purpose of over expression the protein in the selected cells.

• Second method subcellular fractionation was done for the separation of the cellular components.

• Immunoblotting was chosen, as it is important for the identification of proteins based on their masses or weights (Hatano, 2010).

• The main aim of the paper is to analyse the roles of Survivin as an important prognostic factor in the breast cancer patients. Thereby the cells overexpressing surviving were administered the apoptosis inducing agents as Etoposide, Staurosporine or Melphalan.

• Statistical

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