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FEBRUARY 27, 2008

Effect of Allograft Bone Processing on Structural Cortical Grafts: A Comparison of Three Proprietary Processing Methods
Michael G. Dunn, Ph.D.
Associate Professor, Department of Orthopaedic Surgery Director, Orthopaedic Research Laboratories UMDNJ-Robert Wood Johnson Medical School 1 Robert Wood Johnson Place, New Brunswick, NJ 08903

Summary
The objective of this study was to determine the effect of three commercial bone cleaning processes on the bone remodeling characteristics of cortical bone.
Cortical bone is useful for its load bearing properties. Cortical struts are typically used in applications where internal stabilization is desired. The cortical allografts serve as a stabilizing strut while undergoing bone remodeling by the mechanism of creeping substitution. The processing of cortical bone, intended to remove undesirable immunologically active elements, may also remove desirable elements (including endogenous growth factors) and affect the bone’s ability to undergo creeping substitution.

Summary, continued

Results of this study suggest that: • DBM prepared from MTF ATP cortical tissue was consistently osteoinductive in this model; 7 out of 7 ranked samples were osteoinductive (1 sample could not be ranked due to histological artifacts). The average bone score for the 7 ranked samples was 2.17 ± 0.82. • DBM prepared from RTI BioCleanse cortical tissue was not osteoinductive in this model; 7 out of 7 ranked samples were not osteoinductive (1 sample could not be ranked due to histological artifacts). The average bone score for the 7 ranked samples was 0.00 ± 0.00, exactly the same as the heatinactivated negative control. In addition, histological views showed fibrous tissue/inflammatory response. • DBM prepared from LifeNet Allowash cortical tissue was partially osteoinductive in this model; 3 out of 7 ranked samples were

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