...Christian Rodriguez Mrs. Gentry AP Biology September 27, 2015 Lab Abstract Enzymes are proteins produced by living cells. In an enzyme, the substrates bind with the active site of an enzyme, breaking them down into other molecules. Enzymes acts as catalyst which lower the reaction energy and allows it to take place in less time and at lower temperatures. Enzymes can be used in a reaction without actually being used up; therefore, they can be used reversibly. Temperature, salt concentration, substrate concentration, enzyme concentration, and pH can affect enzyme reactions. If the pH is too basic, the enzyme gains (H+) and denaturalizes; likewise if the pH is too acidic, then the enzyme loses (H+) and becomes denaturalized. A major benefit...
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...Optimal Growing Conditions for Russet Potatoes Lauren Mendoza Lab Group: Benjamin Lowe, Winifred Zee, Courtney Machac Introduction The purpose of this lab was to find the optimal growing conditions for Russet potatoes by conducting a series of experiments. Here in this experiment we will look at NaCl concentration, pH levels and wavelengths to determine which ones are more beneficial for growth of Russet Potatoes. In Lab 2.1 we looked at levels of NaCl in hopes to find the isotonic concentration for the potatoes. Plants do prefer hypotonic solutions. Plants prefer hypotonic environments because the water from its environment gets soaked up by the cells then is stored in their vacuoles. The vacuoles will apply pressure to the cell wall which...
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...Unknown Identification Introduction to Microbiology Lab Professor M Biology Lab 1913 11/11/2014 I. Introduction A series of tests was completed in order to identify an unknown microorganism. A gram stain reaction test was done. A gram stain reaction test is used to differentiate between two bacterial species. The two species; gram positive and gram negative bacteria have varying properties of their cell wall structural composition. The gram positive bacteria contain a thick peptidoglycan layer in their cell wall which retains the primary crystal violet stain. The crystal violet is washed from the fixed stain and gram-negative bacteria appear red after the decolorizer washes the primary stain due to their more porous higher lipid content walls and the safranin counter stain adheres to their thinner cell wall. The microscopic examination of the bacteria after staining allows for the morphology of the organism to be determined because as the cell is killed during the staining process it retains its rigid structure allowing for morphology determination. A fermentation test was done. Three different carbohydrates are used to determine whether the organism can ferment a sugar as well as if a gas is produced during heterofermentation. A phenol red broth is used which retains a red color at a pH of 7.4 indicating no fermentation of a sugar. When an acid is produced during fermentation, the pH of the broth will lower and the broth will turn yellow. The sugars...
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...Osmosis is diffusion of water through a selectively permeable membrane. Water will diffuse from the place with higher concentration to the place with a lower concentration. Water goes through the concentration gradient. Which is when molecules without energy diffuse from the place with higher concentration to the one with lower (1). The ability to diffuse through water all depends on the size of the molecule as well. For example, starch is a large molecule therefor it should not fit through the cells membrane. Starch is a polysaccharide because it is comprised of lots of sugars. Individually these glucose molecules are able to pass through the cells membrane but due to the amount of them in starch it can not pass through. Amylase is an enzyme that catalyses the hydrolysis (splitting of a compound by addition of water molecules) of starch into sugars (1). Amylase breaks the starch down into smaller sugar molecules going from C6 H10 O5 to glucose which is C6 H12 O6. The...
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...Rate of Reaction of Catalase Abstract Properties of Enzymes focused on the variations of reaction rates amongst enzymes subjected to various circumstances such as temperature, pH levels, different concentrations of substrate, salt concentrations, Metal Copper Sulfate and lastly, the presence of an Enzyme Inhibitor. The assigned section of this laboratory for our efforts was the effect of temperature variations on enzyme reactions. To perform the experiment, we used a spectrophotometer to monitor the baseline catalase activity when they are placed in these two temperatures. In this way, absorbance can be measured over time to monitor catalase activity of the main baseline reaction. Our results showed that temperatures at higher degrees led to being inactive, whereas those at lower degrees lowered the reaction time. This comes to show that each enzyme can have a different optimal temperature and this experiment helped us to understand how reaction rate can be affected by temperature change. Introduction Thousands of complex biological processes are constantly taking place within our bodies. We require material transport, energy synthesis, and the manufacturing of various proteins, hormones, and other molecules (Source 1). Almost all of these everyday processes rely on the function of enzymes to take place. Enzymes are specifically grouped according to their function, and this information can often provide us with clues regarding what type of reaction that enzyme will catalyze...
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...The Effects of Peroxidase on Enzyme Activity Gianna Crowe Bio Lab 117 October 16th, 2014 Most enzymes are proteins that speed up reactions and are characterized as catalysts. Enzymes work in such a way that when the right chemicals of a molecule are present for the enzyme, it will fully fit the shape. The part of the particular shape is called the active site of the enzyme, since this is where the reaction occurs. The molecule that the enzyme works on is called the substrate. An enzyme reaction includes a substrate (substance) that is converted to another product. The unique shape of the active site of the enzyme allows it to bind with only certain kinds of molecules, which is the substrate of the enzyme (Strobl 2014). A substrate binds to the active site of the enzyme to form a enzyme-substrate complex for a very short time, this then becomes part of a new formation and a new product of a specific reaction is formed then released freeing the active site, allowing the enzyme to repeatedly bind another substrate. Enzymes are produced by all living things, and are a necessity to life. They are responsible for constructing, synthesizing, carrying, dispensing, delivering, and eliminating the many chemicals associated in living organisms (Colpa 2014). An example for how enzymes work in living organisms would be the process of food digestion, enzymes work to break down food and speed up the digestion process. Factors that affect enzyme activity deal with environmental conditions...
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... A pipette (also called a pipet, pipettor or chemical dropper) is a laboratory instrument used to transport a measured volume of liquid. Pipettes come in several designs for various purposes with differing levels of accuracy and precision, from single piece glass pipettes to more complex adjustable or electronic pipettes. Lab dish washing Cleaning laboratory glassware isn't as simple as washing the dishes. Here's how to wash your glassware so that you won't ruin your chemical solution or laboratory experiment. You can rinse the glassware with the proper solvent, then finish up with a couple of rinses with distilled water, followed by final rinses with deionized water Water Soluble Solutions (e.g., sodium chloride or sucrose solutions) Rinse 3-4 times with deionized water then put the glassware away. Water Insoluble Solutions (e.g., solutions in hexane or chloroform) Rinse 2-3 times with ethanol or acetone, rinse 3-4 times with deionized water, then put the glassware away. In some situations other solvents need to be used for the initial rinse. Lab chemical stock maintenance • Remove items from the written inventory as they are disposed or used • Record all new orders • Record the received date on...
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...Introduction Morphological, staining, cultural and biochemical data on the characteristics of different microorganisms was gathered. This gained information can be used to identify bacteria. A scientist by the name of David Bergey was the first person who proposed the system of bacterial classification in which bacteria are grouped according to Gram reaction, metabolism, and morphology. The first edition of Bergey’s Manual of Systematic Bacteriology was published in 1984. This book can be used to identify a microorganism. The purpose of this experiment is to identify an unknown bacteria using the skills learned in microbiology laboratory this semester. Materials and Methods This experiment was conducted at Louisiana State University in Shreveport...
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...clusters of genes together on the genome into operons that elicit a processive response in the presence of a specific metabolite. The Lac operon is responsible for the cleaving of the disaccharide lactose into two products. A myriad of components control the expression of the Lac operon when two conditions are met. First, the substrate, lactose, must be present. Second, no better substrate for example, glucose, is present (2). The three structural genes in the Lac operon are lacZ, lacY, and lacA. The gene lacZ encodes the tetramer, ß-galactosidase, which is responsible for hydrolyzing the ß-1,4 glycosidic linkage between galactose and glucose in lactose. The transport of lactose into the cell via the enzyme lactose permease is encoded by the gene lacY. The lacA gene encodes the enzyme, galactoside transacetylase, a trimer that transfers an acetyl group from acetyl-CoA to galactosides. Activation of these genes is dependent on the activity of a promoter and three operators based on the nutritional and environmental conditions available to the cell. The lac operon is a negatively controlled inducible operon that utilizes the product of the regulator gene lacI, to repress RNA polymerase from transcribing the lacZYA genes. The three operators involved in the lac operon: O1, O2, and O3 serve as binding sites for lacI and when bound repression is exhibited. Originally, the Jacob-Monod model of the Lac operon proposed only one operator existed. However, with the discovery of two additional...
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...Summer Project Report Study of MDA (malondialdehyde) as abiotic stress marker in CSV-17 variety of Sorghum bicolor. Submitted in partial fullfilement of the requirement for B.Tech. Biotechnology Semester VII AMITY INSTITUTE OF BIOTECHNOLOGY AMITY UNIVERSITY RAJASTHAN JAIPUR 2011 Supervised by: Dr Ajit Kumar Sr. Research Officer S.P. Institute of Biotechnology, Jaipur Submitted by: Ravi Pareek DECLARATION I hereby declare that the project report entitled “Study of MDA (malondialdehyde) as abiotic stress marker in CSV-17 variety of Sorghum bicolor” is a record of the work compiled by me under the supervision and guidance of Dr. Ajit Kumar, S.P. Institute of Biotechnology, Jaipur as a part of my 45 days summer training. Ravi Pareek (B.TECH-BIOTECHNOLOGY) (AUR0821094) ACKNOWLEDGEMENT First of all with due regard to my respective god with whose kindness and blessing we could be able to accomplish the task of training. Mr. Sourabh Pareek, for his kind permission to allow me to undergo my major project at S. P. Institute of Biotechnology, Jaipur. I am overwhelmed with rejoice to take this opportunity to evince my profound sense of reverence and gratitude to my esteemed supervisor respective Dr. Ajit Kumar, for giving his regular advice and excellent suggestion which have helped us for completing the study. His regular assistance and guidance really helped me to bring formidable task in successful manner. Sincere thanks to Dr. Sonali Jana and Dr. Neha Upreti for their cooperation...
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...Exp’t 112 Isolation of Casein, Lactose, and Albumin from Milk Adapted by R. Minard (Penn State Univ.) from Introduction to Organic Laboratory Techniques: A Microscale Approach, Pavia, Lampman, Kriz & Engel, Saunders, 1990. Revised 3/20/2000 PreLab Exercise: Draw a mechanism for the acid-catalyzed hydrolysis of the acetal bond in lactose; see reaction below. Hint: your textbook may be of some help; first identify the functional group that is undergoing a reaction and consider the catalyzed used. H HO OH CH2OH O O H OH H2C OH O HO HO OH OH H2C OH O HO HO + HO OH H2O OH CH2OH O HO H + D -Galactose D -Glucose OH α-Lactose: D -Galactose+ D -Glucose Introduction: The Chemistry of Milk Milk is a food of exceptional interest. Not only is milk an excellent food for the very young, but humans have also adapted milk, specifically cow’s milk, as a food substance for persons of all ages. Many specialized milk products like cheese, yogurt, butter, and ice cream are staples of our diet. Milk is probably the most nutritionally-complete food that can be found in nature. This property is important for milk, since it is the only food young mammals consume in the nutritionally significant weeks following birth. Whole milk contains vitamins (principally thiamine, riboflavin, pantothenic acid, and vitamins A, D, and K), minerals (calcium, potassium, sodium, phosphorus, and trace metals), proteins (which include all the essential amino acids), carbohydrates (chiefly lactose), and lipids...
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...reference to the human body. (LECT & LAB) Describe each of the following levels of organization of the human body: chemical, cellular, tissue, system, organism. (LECT & LAB) Identify the principal systems of the body. (LECT & LAB) Describe the standard anatomical position of the human body. (LECT & LAB) Compare common anatomical terms used to describe the various regions of the human body. (LECT & LAB) List by name and location the principal body cavities. (LECT & LAB) Define homeostasis and explain the effects of the lack of homeostasis on the body's function. (LECT & LAB) Describe the chemical level of organization of the human body. Describe the interrelationship of matter, elements, and atoms. (LECT) Identify by name and symbol the principal elements of the human body. (LECT) Diagram and label the structure of a typical atom. (LECT) Describe the function of electrons located in incomplete outer energy levels. (LECT) Define the terms isotope and radioisotope and give their functions. (LECT) Describe the three types of chemical bonding (ionic, covalent, hydrogen) and give an example of each. (LECT) Describe what happens during a chemical reaction. Give three types of chemical reactions. (LECT) Interpret the information given in chemical notations. (LECT) Define ion, oxidation, and reduction. (LECT) Distinguish between organic and inorganic molecules. (LECT & LAB) Define solution, solute, and solvent. (LECT & LAB) Define...
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...IDENTIFICATION OF UNKNOWN BACTERIA It is virtually impossible to identify bacteria based on physical characteristics alone. This is due to the fact that there are only a few basic shapes and physical features commonly seen in the prokaryotic world. Instead, biochemical testing has been used to make bacterial identification down to the “species” level. These schemes are based on creating and matching biochemical profiles of the production of enzymes, acids and gases by isolated pure cultures of a given microorganism. Identification schemes and flow charts can be found in reference texts such as “Bergey’s Manual of Determinative Bacteriology” or “The Prokaryotes”. Each group of students will receive a TSA slant or broth containing a pure culture of an unknown bacterium belonging to the Family Enterobacteriaceae. It is the responsibility of the group to maintain stock cultures of the organism provided. Working stock cultures will be used to inoculate the various biochemical test media over the next several weeks and should be fresh and free from contaminants. A reserve stock culture should be made and after incubation and comparison with the original slant, kept with the original slant in the refrigerator. It is critically important that aseptic techniques are used during transfers and inoculations to prevent contamination of your cultures. If contamination is suspected, you will be able to fall back to your reserve stock. If you fail to maintain a reserve stock...
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...(Protonix) from internet media. Class: Proton Pump Inhibitor (pantoprazole, n.d.). Mechanism of Action: Binds to an enzyme in the presence of acidic gastric pH, preventing the final transport of hydrogen ions into the gastric lumen (pantoprazole, n.d.). Drug Administration and Dosage: PO (Adults): 40 mg once daily. PO (Children 5 yr): 15– 39 kg— 20 mg once daily for up to 8 wk; 40 kg— 40 mg once daily for up to 8 wk. IV (Adults): 40 mg once daily for 7– 10 days. Gastric Hypersecretory Conditions; PO (Adults): 40 mg twice daily, up to 120 mg twice daily. IV (Adults): 80 mg q 12 hr (up to 240 mg/day) (pantoprazole, n.d.). Drug Interactions: Drug-Drug: Maypabsorption of drugs requiring acid pH, including ketoconazole, itraconazole, atazanavir, ampicillin esters, and iron salts. Mayqrisk of bleeding with warfarin (monitor INR/PT). Hypomagnesemiaqrisk of digoxin toxicity. Mayqmethotrexate levels (pantoprazole, n.d.). Labs effects/ interference: May cause abnormal liver function tests, including increased AST, ALT, alkaline phosphatase, and bilirubin. May cause hypomagnesemia. Monitor serum magnesium prior to and periodically during therapy (pantoprazole, n.d.). Special considerations: Contraindicated in Hypersensitivity; OB: Should be used during pregnancy only if clearly needed; Lactation: Discontinue breast feeding due to potential for serious adverse reactions in infants. Use Cautiously in: Patients using high-doses for 1 year (qrisk of hip, wrist, or spine fractures); Pedi:...
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...Chem 200 (8/28) * Chapter 1 and 2 homework ( paper) turn in next week (wed) pg 217 in lab manual * Quiz 1 in discussion sections next week * Connect homework is live -chapter 1 and 2 due sept 6 * Know common elements and ions-lab manual/syllabus pages 14-16 * Read Chapters for exams Notes Errors in measurements * Radom error: all measurements have some level of random error they can be either positive( measure more than you actually do ) or negative errors( measure less then you usually do ) * Systematic error: always the same direction (either positive or negative) Precision and Accuracy * Precision : how reproducible each measurement is , how close each measurement is to the other measurements * Accuracy : how close the measured values are to the true value. Temperature Conversions (on the exam) Chemical Language * Elements (Na,Cl)- letters of the language * Chemical Formulas (NaCl)- words of the language Water Vs. Hydrogen Peroxide (on the exam) * The use of Hydrogen peroxide: nuclear, bomb, bleach hair,etc * Molecular formula : water: H2O, Hydrogen Peroxide : H2o2 * Empirical Formula: water HO, Hydrogen peroxide: HO * Structural Formula : water HOH, hydrogen peroxide: HOOH quetion: how many atoms of carbon, hydrogen, and oxygen are present in a single molucule of citric acid C3H5O(CO2H)3 Carbon: 6 Hydrogen :8 Oxygen: 7 Chemical Bonding * Bonds form between atoms when they share electrons *...
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