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Scoop 10 Algae Transfer Pipets

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The procedure also as follows, the experiment needs to be done at nearly exact if optimal results are wanted. First label a cuvette light and another dark. Make sure light can still reach the algae beads. Then convert a pipet into a scoop by cutting diagonally on the 100 ul mark. Label it algae transfer pipet and use it to scoop 10 algae beads into the dark and light cuvettes. Label a new pipet excess and use it to remove and discard the liquid transferred along with the beads. Next label a new transfer pipet wash, and use it to add 1 ml of distilled water to each of the cuvettes. Let the algae beads incubate for 5 minutes to allow indicator in the bead to wash out. After, use the wash pipette to remove the water from the cuvette and discard

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