...Laboratory 3: Molarity Of Saline Solution Note: Lab reports are to be completed by each student individually and in their own words Data: Please write your observations about the appearance of the solution. 1. Normal Saline Mass of NaCl: .9/100 ml x 100= .009 .009x100=.9 Observation: clear, completely dissolved 2. Nasal Irrigation Saline Mass of NaCl: 1.1g Mass of NaHCO3: .3g Observation: clear, completely dissolved, when mixing I was a little cloudy 3. Nasal Irrigation Saline 29.7 Observation, This solution was very cloudy with bubbles, did not dissolve all the way Results: 1. Determine the molar mass of NaCl. Show the workup. Na= 23 plus C= 35 = 58 NaCl molecules 58g/m NaCl 2. Determine the molarity of the two solutions you prepared in terms of NaCl. Show the workup. (a) Normal Saline - Molarity of NaCl M= .9 mole NaCl / 0.1 L solution= 9m NaCl (b) Nasal Irrigation Saline - Molarity of NaCl 118ml = 0.118L M= 1.1g/mole NaCl / 0.118L solution = 9.322 M NaCl (c) Nasal Irrigation Saline - Molarity of NaHCO3 M= .3 mole NaHCO3 / o.118L = 2.54 M NaHCO3 3. The University of Wisconsin recommends that if stinging or burning occurs, than individuals should cut the amount of salt in half for the solution and decrease frequency of use to every other day. How does that affect the concentration of the solution? When adding less salt it make the solution to become more dilute solution...
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...department at the university of texas at dallas, RICHARDSON, TX 75080 march 2016 [Company name] | [Company address] ------------------------------------------------- γ-globin analysis by expression profiling through RT-qPCR, quantification through ELISA, and oxidative stress management analysis by FACS from KU812F cells under treatment by δ aminolevulinic acid, succinylacetone, and N-methyl mesoporphyrin Shaan Sarode, Jose Cordero, and Dr. Li Liu experiments in this paper done through instruction in graduate course: Biotechnology laboratory in the natural sciences and mathematics department at the university of texas at dallas, RICHARDSON, TX 75080 march 2016 [Company name] | [Company address] ------------------------------------------------- γ-globin analysis by expression profiling through RT-qPCR, quantification through ELISA, and oxidative stress management analysis by FACS from KU812F cells under treatment by δ aminolevulinic acid, succinylacetone, and N-methyl mesoporphyrin Shaan Sarode, Jose Cordero, and Dr. Li Liu ABSTRACT Hemoglobinopathies refer to a group of blood related disorders that encompass important disease such as thalassemia and sickle cell disease. Because many of these disease are hereditary more aggressive genetic therapies are showing promise as possible avenues of treatment. One such method is to re-express fetal hemoglobin (HbF) in hopes that it will take over the role as main functional hemoglobin. In this paper we aim to build upon previously...
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...Lab Manual Introductory Biology (Version 1.4) © 2013 eScience Labs, LLC All rights reserved www.esciencelabs.com • 888.375.5487 2 Table of Contents: Introduc on: Lab 1: Lab 2: Lab 3: Lab 4: The Scien fic Method Wri ng a Lab Report Data Measurement Introduc on to the Microscope Biological Processes: Lab 5: Lab 6: Lab 7: Lab 8: Lab 9: The Chemistry of Life Diffusion Osmosis Respira on Enzymes The Cell: Lab 10: Lab 11: Lab 12: Lab 13: Lab 14: Lab 15: Cell Structure & Func on Mitosis Meiosis DNA & RNA Mendelian Gene cs Popula on Gene cs 3 4 Lab Safety Always follow the instruc ons in your laboratory manual and these general rules: eScience Labs, LLC. designs every kit with safety as our top priority. Nonetheless, these are science kits and contain items which must be handled with care. Safety in the laboratory always comes first! Lab Prepara on • • Please thoroughly read the lab exercise before star ng! If you have any doubt as to what you are supposed to be doing and how to do it safely, please STOP and then: Double-check the manual instruc ons. Check www.esciencelabs.com for updates and ps. Contact us for technical support by phone at 1-888-ESL-Kits (1-888-375-5487) or by email at Help@esciencelabs.com. • Read and understand all labels on chemicals. If you have any ques ons or concerns, refer to the Material Safely Data Sheets (MSDS) available at www.esciencelabs.com. The MSDS lists the dangers, storage requirements, exposure treatment...
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...link LAC+USC with its network of 208 primary care clinics (PPP). The purpose of this paper is to describe a product which will provide state-of –the art Electronic Medical Records. Provide an explanation on why the Epic EMR software is needed, what Epic’s EMR impact will have on LAC+USC and the network of clinics under the umbrella of LAC+USC. The writer will also discuss the social, ethical and economic impact Epic EMR software would have on LAC+USC network. LAC+USC data Los Angeles County - USC Medical Center (LAC+USC) is located in East Los Angeles, California. “In one year, the hospital will treat close to 800,000 patients, deliver 10,000 babies, treat 250,000 people in its emergency room, treat about half of all AIDS and Sickle Cell patients in Southern California, and handle 5,000 outpatient visitors per day” ("Los Angeles Almanac", 1998-2012). Due to the large volume of patients treated at LAC+USC, LAC+USC relies on a network of 208 contracted clinics known as Public Private Partnership (PPP) to provide primary care services. The 208 PPP clinics generate the most referrals to LAC+USC emergency room or specialty clinics for services. Patients referred to LAC+USC for specialty services range from homeless, mentally ill, new immigrants and drug addicts. Many...
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...Karen Bleile Genetic Report for Designer Babies Biology 111-112 / J. Thomson October 30, 2012 In the 21 century the term designer babies made the transition from sci-fi movies into the real world where it is defined as “a baby whose genetic makeup has been artificially selected by genetic engineering combined with invitro fertilization to ensure the presence or absence of particular genes or characteristics.” (Oxford University, 2005) Breeders of animals and plants have been using this technique to “produce organisms that will possess desirable characteristics, such as high crop yields, resistance to disease, high growth rate and many other phenotypical characteristics that will benefit the organism and species in the long term.” (Butler, 2012) We need to pause and ask ourselves if we start to cross breed the human race what are the moral or ethical limits, if any, and should we apply guidelines in allowing people to choose their children’s genes or characteristics when designing a baby. In many science fiction movies the subject of having genetically modified humans or creatures are often brought up. The creative author has the ability to give their characters any traits they want. They have the power to make the characters stronger, faster, more intelligent, to specify how they look, or simply be superhuman. Now imagine the scenario if the stories cease to lie in just the films we...
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...Pulmonary Problems Chapter 10 Pneumonia is inflammation of the lungs resulting in consolidation Obstruction in gas exchange on the alveolar level Aspiration most common cause 2nd most frequent cause is droplet inhalation Least likely cause is blood borne Protein rich fluid move into the alveoli- complicated by production of the organism- result is decreased alveolar surface area-resulting in elevation of pco2 and a decrease in po2 Inspection-tachypnea and central cyanosis Percussion-dullness Palpitation-tactile fremitus Auscultation-crackles, rhonchi, wheezing, or egophony Diagnosis-labs, chest xray Ct not recommended unless anthrax is suspected Bronch-immunocompromised individuals and patients who have not responded to treatment Xrays Focal- bacteria Interstitual-viral Rapid progression/ multifocal- legionella, pneumococci, staphylococci Medialstinal widening without infiltrates- inhalation anthrax Tamiflu within 48 hours to be effective HAP-received care in a health care institution for at least 2 days in the last 90 days prior to infection VAP- pneumonia within 48 hours of intubation HAP more difficult to treat compared to CAP COPD- include emphysema, chronic bronchitis, and small airway disease Emphysema-structural change via destruction and enlargement of alveoli Chronic bronchitis- chronic cough and mucous production Small airway disease-generalized narrowing of bronchioles, may include asthma COPD-4th leading cause of death in United States ...
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...was twenty years ago. It is a method that involves the production of a group of identical cells or organisms that all derive from a single individual (Grolier 220). It is not known when or how cloning humans really became a possibility, but it is known that there are two possible ways that we can clone humans. The first way involves splitting an embryo into several halves and creating many new individuals from that embryo. The second method of cloning a human involves taking cells from an already existing human being and cloning them, in turn creating other individuals that are identical to that particular person. With these two methods almost at our fingertips, we must ask ourselves two very important questions: Can we do this, and should we? There is no doubt that many problems involving the technological and ethical sides of this issue will arise and will be virtually impossible to avoid, but the overall idea of cloning humans is one that we should accept as a possible reality for the future. Cloning humans is an idea that has always been thought of as something that could be found in science fiction novels, but never as a concept that society could actually experience. "It is much in the news. The public has been bombarded with newspaper articles, magazine stories, books, television shows, and movies as well as cartoons¨, writes Robert McKinnell, the author of Cloning: A Biologist Reports (24). Much of this information in these sources leads the public in the wrong direction...
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...Unit II: Genetics Brief Overview Reading: Chapters 3, 4, 9-12, 14 (Note: you have reviewed much of this already) The earth is teeming with living things. We can easily see some of the larger organisms—trees, grass, flowers, weeds, cats, fish, squirrels, dogs, insects, spiders, snails, mushrooms, lichens. Other organisms are everywhere, in the air, in water, soil and on our skin, but are too small to see with the naked eye—bacteria, viruses, protists (single celled eukaryotes such as amoebae), and tiny plants and animals. Life is remarkable in its complexity and diversity, and yet it all boils down to a very simple idea—the instructions for making all this life are written in nucleic acids, usually DNA. Most organisms have a set of DNA that contains the instructions for making that creature. This DNA contains four “letters” in which these instructions are written—A, T, G, and C. The only difference between the code for a dog and the code for a geranium is in the order of those letters in the code. If you took the DNA from a human and rearranged the letters in the right way, you could produce an oak tree—arrange them slightly differently and you would have a bumble bee—arrange them again and you would have the instructions for making a bacterium. Acting through more than two billion years, the process of evolution has taken one basic idea—a molecular code that uses four letters—and used it over and over, in millions of combinations to produce a dazzling array of life forms...
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... 1. Q: A 48 y/o alcoholic male presents to your primary care office c/o diarrhea that began 1 week ago. Patient admits to eating fast food 3 times day. During the exam you note yellow sclera and glossitis, lab findings show MCV>100. What are you suspecting to be the cause of his symptoms? a) folate deficiency b) B12 deficiency c) hereditary spherocytosis d) thalassemia e) lipid deficiency A 2. 67 yo homeless, white male patient was brought to ED by local EMTs. Pts stuporous, HR: 120, regular BP: 160/90 and RR: 10 non-labored. While inspecting the patient, you notice upper extremity peripheral jaundice, rosacea, finger clubbing and rhinophyma. On futher exam, JVD and LE edema is visible with palpable splenomegaly and hepatomegaly. As a part of your work up, you order a CBC with diff, which reveals normocytic, hypochromic red cells and a decreased reticulocyte count. Your CBC work up reveals the patient's likely cause of anemia. ANSWER: alcoholic with anemia of chronic disease. 3. Pregnant 28 year old female brought into the ER by her husband because she was exhibiting neuro symptoms of stupor, bloody diarrhea, and abdominal pain. On further observation patient is in renal failure, has microangiopathic hemolytic anemia, and severe HTN. You ran labs on your patient and discovered she has low thrombocytes, low haptoglobin, high reticulocyte count, high LDH and creatinine, schistocytes on peripheral smear. What would you diagnose the patient with? Answer:...
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...University of Central Florida College of Medicine Obstetrics and Gynecology Clerkship Revised 5/4/11 lab 1 | P a g e University of Central Florida College of Medicine Obstetrics and Gynecology Clerkship Revised 5/4/11 lab 2 | P a g e TABLE OF CONTENTS GENERAL INFORMATION ‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐ 4 RECOMMENDED TEXTS/RESOURCES ‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐ 5 DIDACTIC EXPERIENCE ‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐ 6 IMPORTANT LOCATIONS ‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐ 7 OBSTETRICAL SERVICE: FLORIDA HOSPITAL AND WINNIE PALMER HOSPITAL ‐‐‐‐‐‐‐‐‐‐‐‐ 8 GYNECOLOGIC AND GYNECOLOGIC ONCOLOGY SERVICES: FLORIDA HOSPITAL AND WINNIE PALMER HOSPITAL ‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐ 11 COMMUNITY WEEK AND SPECIALTY CLINICS‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐14 TIPS FOR A SUCCESSFUL OB/GYN ROTATION ‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐ 15 LOGGING PATIENT ENCOUNTERS (THE PATIENT PASSPORT) ‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐ 16 CLERKSHIP ATTENDANCE POLICY ‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐ 19 CLERKSHIP OBJECTIVES ‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐ 20 THE CASE...
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...INSTRUCTOR GUIDE Human Anatomy & Physiology Laboratory Manual MAIN VERSION, Eighth Edition Update CAT VERSION, Ninth Edition Update FETAL PIG VERSION, Ninth Edition Update ELAINE N. MARIEB, R.N., Ph.D Holyoke Community College SUSAN T. BAXLEY, M.A. Troy University, Montgomery Campus NANCY G. KINCAID, Ph.D Troy University, Montgomery Campus PhysioEx™ Exercises authored by Peter Z. Zao, North Idaho College Timothy Stabler, Indiana University Northwest Lori Smith, American River College Greta Peterson, Middlesex Community College Andrew Lokuta, University of Wisconsin—Madison San Francisco • Boston • New York Cape Town • Hong Kong • London • Madrid • Mexico City Montreal • Munich • Paris • Singapore • Sydney • Tokyo • Toronto Editor-in-Chief: Serina Beauparlant Project Editor: Sabrina Larson PhysioEx Project Editor: Erik Fortier Editorial Assistant: Nicole Graziano Managing Editor: Wendy Earl Production Editor: Leslie Austin Composition: Cecelia G. Morales Cover Design: Riezebos Holzbaur Design Group Senior Manufacturing Buyer: Stacey Weinberger Marketing Manager: Gordon Lee Copyright © 2009 Pearson Education, Inc., publishing as Pearson Benjamin Cummings, 1301 Sansome St., San Francisco, CA 94111. All rights reserved. Manufactured in the United States of America. This publication is protected by Copyright and permission should be obtained from the publisher prior to any prohibited reproduction, storage in a retrieval system, or transmission in any form or by any means...
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...the following anemias is caused by a failure of the bone marrow to produce red blood cells and may be congenital or acquired? A. Bone marrow deficiency anemia B. Sickle-cell anemia C. Aplastic anemia D. Thalassemia 2. Which of the following terms refers to either a reduction in the quantity of hemoglobin or a reduction in the volume of red blood cells? E. Anemia F. Coagulation defect G. Thrombocytopenia H. Leukocytosis 3. When a diagnostic statement of anemia is not qualified in any way, what should the coder do? I. Review the record for a surgical procedure; and if the patient had surgery, code as anemia due to acute blood loss. J. Review the medical record before assigning an unspecified type of anemia. K. Review the medical record to determine if the patient has been prescribed iron tablets, and code as iron-deficiency anemia. L. Review the medical record to determine if the patient has received chemotherapy, and assign the code for anemia due to antineoplastic chemotherapy. 4. Which of the following statements is true regarding a patient with documented sickle-cell trait? M. The patient has sickle-cell disease. N. Both of the patient’s parents carried the sickle-cell gene. O. The code for sickle-cell disease should be assigned. P. The patient received the sickle-cell gene from only one parent and is a carrier of the trait. 5. How should hemorrhagic...
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...Nursing 344 Week 1: Report Writing & GFHP Report Writing Pt records are sometimes called in evidence before a court of law in order to establish events that may have contributed to a pt’s death or injury. They may also be required as evidence for an inquiry or hearing by the NMB of NSW. ▪ Frequency of documentation relies on: - physical/ mental status of the pt. - the type of care provided (self care v. intensive care) - requirements of health care agency - any legal or other obligations that the health record must meet ▪ Content of documentation needs to be: - relevant - appropriate - accurate - requirements will vary according to pt acuity - content may be guided by framework (assessment, intervention, response) ▪ Documentation framework: - assessment: conclusions reached utilising subjective and objective data - intervention: reflects the action taken - response: reflects the pt’s response to the intervention ▪ Example of using framework to case: Mrs Pat Martin, a 28y/o lady has been admitted overnight via Casualty accompanied by husband. She is 16 wks pregnant and has been diagnosed with appendicitis by Dr Chan. At the moment she is only experiencing mild pain and has a low grade fever of 37.7. IMI Pethidine 50mgs was given at 2am, and may be repeated PRN. Shes been added to the theatre list at 9.30am. She remains NMB and has IV normal saline running over 10hrs. IV Keflin QID commenced, is next due 12md...
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...Diarrhea / ORT DEFINITION | SIGNS/SYMPTOMS | Most commonly due to acute infectious diarrhea (gastroenteritis) from a variety of causative organismsAntibiotics are a common cause of acute diarrhea! | Acute inflammation of the lining of the stomach and intestines caused by viruses, bacteria or their toxins or parasitesPresents commonly with diarrhea, abdominal cramps, and vomiting.CommunicabilityOften fecal-oral route (especially viruses)Food poisoning (especially bacteria)Day care centers, crowded living conditions, poor sanitation and cleanliness | DIAGNOSTIC TESTS | MAIN TREATMENT | Stool gram stain and culture (if bloody stools)Stool for Ova & Parasites (if hx suggestive)CBC – assess for anemia/infectionUrinalysis and urine culture (r/o UTI)Electrolytes | Oral rehydration therapy (ORT) is one of the major worldwide health advances of the last decade:Safer, less painful, and less costly than IV rehydrationOral rehydration solution enhances and promotes reabsorption of H2O and NaReduces vomiting, diarrhea, and duration of illnessORT GuidelinesDiarrhea w/o dehydrationMild dehydrationMod dehydrationSevere dehydrationReplacing ongoing losses | MAJOR TEACHING POINTS | NURSING CONSIDERATIONS | Teach parents at well childcare visits in first yearKeep 24 hour supply of ORT in homeBegin with first sign of diarrheaReplace with ½ cup ORT for each diarrheal stoolSeek medical attention prn signs or dehydrationAfter re-hydration resume breast/formula feeding or normal diet...
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...DO NOT delegate what you can EAT! E - evaluate A - assess T - teach addisons= down, down down up down cushings= up up up down up addisons= hyponatremia, hypotension, decreased blood vol, hyperkalemia, hypoglycemia cushings= hypernatremia, hypertension, incrased blood vol, hypokalemia, hyperglycemia No Pee, no K (do not give potassium without adequate urine output) EleVate Veins; dAngle Arteries for better perfusion A= appearance (color all pink, pink and blue, blue [pale]) P= pulse (>100, < 100, absent) G= grimace (cough, grimace, no response) A= activity (flexed, flaccid, limp) R= respirations (strong cry, weak cry, absent) TRANSMISSION-BASED PRECAUTIONS: AIRBORNE My - Measles Chicken - Chicken Pox/Varicella Hez - Herpez Zoster/Shingles TB or remember... MTV=Airborne Measles TB Varicella-Chicken Pox/Herpes Zoster-Shingles Private Room - negative pressure with 6-12 air exchanges/hr Mask, N95 for TB DROPLET think of SPIDERMAN! S - sepsis S - scarlet fever S - streptococcal pharyngitis P - parvovirus B19 P - pneumonia P - pertussis I - influenza D - diptheria (pharyngeal) E - epiglottitis R - rubella M - mumps M - meningitis M - mycoplasma or meningeal pneumonia An - Adenovirus Private Room or cohort Mask 1 CONTACT PRECAUTION MRS.WEE M - multidrug resistant organism R - respiratory infection S - skin infections * W - wound infxn E - enteric infxn - clostridium difficile E - eye infxn - conjunctivitis SKIN INFECTIONS VCHIPS ...
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