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Tendon And Ligament Injury Case Study

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Specific Aims.
Tendon and ligament injuries are one of the most common orthopedic injuries. An estimated 1 in 1,000 persons injures their Anterior Cruciate Ligament (ACL) per year in the United States.
The ACL does not heal spontaneously, most likely due to the lack of vascularization, growth factors and extracellular proteins that promote healing, thus requiring surgical intervention to restore knee function. Suturing the ruptured ACL is not always sufficient to promote healing, therefore the gold standard of care is a patellar tendon graft reconstruction [1]. While this surgery allows the patient to regain knee function, even with repair, patients experience early onset osteoarthritis, donor site morbidity, and potentially graft failure [2]. …show more content…
Collagen Formation and Vascularization – Histology.
Histological results will be analyzed and compared in specific regards to collagen content, collagen type distribution, vascularity, and number of cells. Images gathered from light and fluorescence microscopy will be assessed with ImageJ for quantification of differences in parameters. Specifically, the total collagen area, ratio of Type I and Type III collagen areas, and number of cells present will be quantified and compared. Additional visualization with FT-SHG will be performed and coupled with ImageJ to quantify differences in collagen fiber orientation
[19]. Results will be compared between the two groups, as well as with healthy ACLs from the opposite knee.
TDSC Differentiation – Immunofluorescence.
Fluorescence microscopy will be used to determine the relative amount of each cell type in the tissue sample. Additionally, the relative distribution of the different cell types within the structure can also be noted. Results will be compared quantitatively using ImageJ between the two groups, as well as with healthy ACLs from the opposite knee.
Expected outcomes.
The TDSC-wrapped group is expected to experience enhanced healing compared to the …show more content…
Additionally, at the later time points, failure of the TDSC sheet may not occur directly at the suture repair site, and failure should be similar to that of the control non-surgically altered group. The TDSC wrapped ACL will have a higher ultimate strength and high Young’s modulus then the suture repair group, and in late stage healing will have a similar ultimate strength and Young’s modulus to the control non surgically altered group.
Anticipated problems and alternative approach.
It is possible that when the complex is being surgically removed or while it is being loading into the test machine the ACL will rupture. This should not be a problem because our sample size is large enough and will still be statistically significant as long as no more than 2 ruptures per group at each time point.
Future work.
Following the successful completion of the research outlined by this grant, future experiments using the method above could be performed in a larger animal model, like a canine model, to better approximate conditions experienced by humans. Long-term studies can also be performed to assess the incidence of osteoarthritis in TDSC-suture repaired ACLs compared

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