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The Infectious Patient

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The infectious patient

If you have an infection how do you know from what bacteria is came from. I will discuss the genera Bacillus, Escherichia and Mycoplasma. They have many structural differences that can distinguish them. There are also staining processes that can identify each. Let’s get started. The genera Bacillus is a gram positive rod shaped bacterium. When viewed under a microscope Bacillus can be seen as a single organism or in chains. It forms endospores that are resistant to heat, cold, radiation and disinfectants. Bacillus is also aerobic, it needs oxygen to grow. Bacillus can be the cause of infections including abcesses, wound, burn and ear infections, endocardistist, meningitis, ophthalmitis, osteomyelitis, peritonitis and respiratory and urinart tract infections. The genera Escherichia is a gram-negative rod shaped bacterium. It does not form endospores. They are anerobic and can survive with or without oxygen. Escherichia can normally be found in the lower intestines. This can be the cause of gastroenteritis and urinary tract infections. The genera Mycoplasma are also rod shaped, like Bacillus and Escherichia. Mycoplasma lack a cell wall which makes staining difficult. It is also so small it makes it undetectable with a normal light microscope. This can be the cause of lung infections and urogenital infections. Infections caused by Mycoplasma are not easily treated by antibiotics. A way to distinguish these genera is by staining. This is a procedure developed by Hans Christian Joachim Graham, a Danish bacteriologistis. This process became known as Gram’s stain or the Gram stain. Gram staining is the coloring of microorganisms with dye so certain structures are seen more easily. To stain a microorganism it must be fixed or attached to the slide so it does not wash away. The microorganism is spread across a slide and

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