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Unknown Lab Report

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Discussion: The purpose of this experiment is to identify an unknown organism over three weeks by performing lab exercises that were previously performed. The unknown #16 was chosen for this experiment. During the first week,
The next step in determining this unknown was to perform the streak plate method for isolation of colonies of a 24-hour period in a 37o C incubator. The unknown organism grew on the media; observed white in color, raised with a smooth margin, shiny colonies on the TSA agar plate.
The next step in identifying this unknown #16 was to steak for isolation on plated media; then incubated at 37o C for 24 hours and the stores at 4o C. The plates used for isolation were; Triple Salt Agar (TSA), Mannitol Salt Agar (MSA), Phenyl …show more content…
The process was to inoculate all biochemical tests and plates with bacteria from circled, isolated colonies on the TSA streak plate. Phenyl Red Broth (PR)-Glucose, PR broth-Lactose, Sucrose, Simmons Citrate Slant, Urea Broth, Gelatinase Stab, Triple Sugar Iron Agar (TSI) Slant, Motility, Nitrate broth, Tryptone both, and MR-VP broth test were inoculated and incubated for 24 hours at 37o C. After the 24 hour incubation period, observations were made. The Urea broth was gold and positive. Glucose broth was observed as yellow, positive and acidic. The Simmons Citrate Slant was observed blue and positive. Due to the slant changing to the color of blue it was positive. The sucrose broth observed as yellow and positive. The lactose was observed as pink, which was positive and acidic. Nitrate broth was observed cloudy light yellow or red; the nitrate reduction was performed, which includes incubation of the nitrate broth then add eight drops of reagent A and eight drops of reagent B and mix well. Tryptone (Indole) was observed as light yellow and fermented. MR-VP was not observed. The motility was observed as cloudy and moving, which made it positive. Gelatin was observed remained gelatin, which made it negative and no change. On the TSI slant was observed as Red/yellow the colors were separated, which made this positive

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