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Uv Light Effect and Repair Lab Report

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Lab Report 3: Effect of UV Light on Microbial Growth
Kristin Holmes – April 2, 2013
PURPOSE: The purpose of this lab is to determine the effects of ultraviolet light on microbial growth and the effectiveness of the repair mechanisms of light repair and dark repair on UV damage.
INTRODUCTION:
Can Ultraviolet (UV) light be a viable form of sterilization and/or disinfection? This lab experiment will look to examine and answer that question. UV light is a form of electromagnetic radiation that is invisible to the human eye. It has a short wavelength and is considered high energy which allows it to pass through some materials. The biological effects are potentially devastating based on the length of exposure and the length of the wavelength exposed to. The reason UV light can be so detrimental is due to its effect on DNA and the mutations that can occur because of exposure. The absorbance of UV photons causes the formation of pyrimidine dimers; these in turn create challenges to DNA replication. While DNA repair mechanisms can remove these dimers, with increased exposure and/or repeated exposure as well as incomplete repair, DNA replication is not always exact. (Aishwariya)
UV radiation is typically placed into one of three categories. UV-A radiation is the longest wavelength and has the least damaging effect. UV-B radiation is medium length and UV-C is the shortest wavelength. (Aishwariya) UV-A radiation can have long term effects; however the most damage, on the cellular level, is caused by UV-C radiation due to the amount of radiation absorbed by the cell’s DNA. (Menard). This DNA damaging capability makes it a potentially very effective bactericidal option, especially for items which cannot be autoclaved.
The main reason that plants, humans & animals, as well as bacteria & viruses do not get annihilated by light to moderate and even sometimes severe

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