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White Eyed Fruit Flies Lab Report

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RESEARCH QUESTION: What will be the phenotypic ratio outcome of and F2 generation of red eyes fruit flies crossed with white eyed fruit flies?

BACKGROUND INFORMATION: sexually dimorphic
. Law of independent assortment Drosophila Melanogaster, the fruit fly completes its life cycle in 10-12 days, makes a large number of offspring, and it has many types of hereditary variations that can be seen with low power microscopes. Drosophila has four pairs of chromosomes, a relatively small number which are easily located in the large salivary glands. Many factors combine to affect the length of the Drosophila life cycle. Temperature affects the life cycle the most. At room temperature the average life cycle of the Drosophila is about 12 days.

HYPOTHESIS: That in a sex-lined cross between …show more content…
Begin by obtaining a vial of wild type flies.
2. Next, these are the steps for immobilizing the flies. Hold the vial containing the flies at an angle and place it in a refrigerator for several minutes. When the flies are immobilized, place them into a small plastic Petri dish.
3. Use the dissecting microscope to view the flies. Make sure to top the petri dish on when viewing the flies.
4. You can easily distinguish male flies from females by looking for the following characteristics: males are usually smaller than the females, males have dark blunt abdomens and females have lighter pointed abdomens. The males have sex combs, which are black bristles on the uppermost joint of the forelegs.
5. Next, get a vial containing experimental flies. Make sure to write down the number of the vial that you have.
6. The flies you now have are the P1generation. The females should have laid eggs. The eggs and larvae are the F1generation. Then after there are eggs present knock out remove the adult flies from the vial.
7. Sex the adult flies and write down any mutations. Place the flies in the morgue that contains alcohol. Make sure to label the vial with the symbols for the

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