...GOLD BUYING PROCEDURE Undocumented/treasure AU Bars PRIOR OR BEFORE IMPLEMENTATION A TABLE MEETING IS A MUST FOR M.O.A SIGNING BY BUYER & SELLER. For sponsored project (Seller is not the one paying the Contingency Fee/ Intermediary/ies paid the fee ) both Parties have agreed that a day before implementation, First Party (SELLER ) will hand over the _____ grams of sliced gold grams from subject gold bars to Second Party ( BUYER ) as “commitment” and to be given to the buyer’s verifier at the venue a day prior ( before ) to implementation as “COMMITMENT” rest in three MAJOR reasons: Assurance gold slice quantity varies as follows : METRO MANILA 100 grams LUZON 200 grams VISAYAS & MINDANAO 300 grams. 1. Avoid last minute back out. ( pag-urong ng seller sa araw ng bilihan / paglabag sa pinirmahang kasunduan ) 2. Avoid switching ( pagpalit ng item na peke ) 3. Serve as key for the Buyer as assurance for expenses incurred ( in caase of seller’s failure ) ( ang sliced grams of gold ay magsilbing pantapal sa nagastos ng BUYER, kung sakali man magkaroon ng hindi pagkatuluyan sa transakayon sa anumang dahilan nilabag ng SELLER sa araw ng buying/implementasyon. ) MIMINUM BUYING PER IMPLEMENTATION 6.2 KG. ( 5 BARS ) 12.5 KG ( 3 BARS ) 74.6 KG. ( 1 BAR ) Transaction/s wherein the Seller or intermeiaries pays for the Contingency Fee for its implementation...
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...culture, is the removal of cells from an animal or a plant, and growing the cells in a favourable artificial condition (Wiley, J.M et al, 2013). There are three types of cell culture: primary, this is cultivating the cells away from the parental tissue, secondary, which is when a primary culture is sub-cultured and cell lines, which can be continuous or finite depending on the life span of the culture. Virus titrations are used to estimate the virus concentration; it is a viral quantification technique. When detecting the virus, the cytopathic effect is looked at, whether there is lysis of the cells, vacuolation, formation of syncytia and the presence of inclusion bodies. TCID50, is the measure of the infectious titre. The end point dilution assay quantifies the amount of virus that is required to kill 50% of infected hosts or to produce a cytopathic effect in 50% of inoculated tissue culture cells (Kumar P, 2013). The purpose of the virus titration within tissue culture is to isolate and identify viruses within clinical samples, to carry out research on the viral structure, replication, genetics of the virus and the effect on the host cells, and also to prepare viruses for vaccine production. Results Table 1 Data used to determine the 50% endpoint using the Reed-Muench method Log of virus dilution | Infected test units | Cumulative infected (A) | Cumulative non-infected (B) | Ratio A/(A+B) | Percentage infected (%) | -1 | 5/5 | 37 | 0 | 38/38 | 100 | -2 | 5/5 | 32 | 0...
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...Introduction Ash is a vital ingredient in the canine diet as it is the main constituent of minerals within most diets. Ash content in food is determined by burning the food to calculate the ash percentage left behind. A deficiency in any of the minerals within ash would result in a wide range of health problems which is why it is a legal requirement to display the ash content on food packaging. This experiment is designed to recreate the process undergone by the pet food company to determine the ash content within the food. Method Weigh silica crucible and record weight prior to experiment. Grind over 5g of food sample in pestle and mortar and measure 5g of ground sample into the crucible. (W0) Place the dish in a baking oven for 24hrs to dry out the sample. Reweigh the sample in silica crucible, This is now the true weight of the sample (W1) Set up the bunsen burner on top of the heatproof mat with tripod and clay pipe triangle above to support the crucible. Ignite the bunsen burner and begin to heat the sample slowly ensuring the sample does not set on fire as this will result in a loss of material to the atmosphere in the form of particles. The sample should turn black. Once the sample reaches roughly 100°C continue to heat to 500°C until the sample turns white. Allow sample to cool to room temperature in a dessicator. Weigh the finished sample (W2) Calculate the ash content (X): (W2-W0)/(W1/W0)x100=X Materials: Silica dish Baking Oven Bunsen Burner ...
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...4110065A.qxp 9/25/2007 2:39 PM Page 1 ™ Quick Start Bradford Protein Assay Instruction Manual For technical service call your local Bio-Rad office, or in the US, 1-800-4BIORAD (1-800-424-6723) 4110065A.qxp 9/25/2007 2:39 PM Page 5 Table of Contents Section 1 Introduction 1 1.1 Principle 1 1.2 Selecting a Protein Standard 5 1.3 Product Description Section 2 Instructions 9 11 2.1 Standard Assay Protocol 11 2.2 Microassay Protocol 14 Section 3 Data Analysis 18 Section 4 FAQs and Troubleshooting 22 Section 5 Ordering Information 26 Section 6 References 28 Section 7 Appendix 30 4110065A.qxp 9/25/2007 2:39 PM Page 7 Section 1 Introduction The Quick Start Bradford protein assay is a simple and accurate procedure for determining the concentration of protein in solution. It provides ready-to-use convenience by supplying the dye reagent at 1x concentration and two protein assay standards at seven prediluted concentrations. The prediluted standards are conveniently packaged in 2 ml screwcap vials, eliminating wasteful and sharp ampoules, and ensuring protein stability over the shelf life of the product. 1.1 Principle The Bradford assay is a protein determination method that involves the binding of Coomassie 1 4110065A.qxp 9/25/2007 2:39 PM Page 8 Brilliant Blue G-250 dye to proteins (Bradford 1976). The dye exists in three...
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...Animal Nutrition Lab Reports FdSc: Animal Health and Welfare STE09288743 Practical 1 - Determination of the ash content of foods Introduction Foodstuffs are divided into five categories; crude protein – the total level of nitrogen in a feed, ether extract – the total fat content of a feed, nitrogen free extract – the soluble carbohydrate in a feed, crude fibre - cellulose, fibre with nitrogen attached & alkali insoluble lignin and total ash content – an indication of the mineral content of feeds. The aim of this report is to try and find out the total ash content of Wainwrights Adult Complete Dog Food With Duck And Rice through a food analysis method and compare the amount found with what the original packaging states. Mclements (2003) explains that “ash is the inorganic residue remaining after the water and organic matter have been removed by heating in the presence of oxidizing agents, which provides a measure of the total amount of minerals within a food.” It’s important for the correct amount of ash to be in a dog’s diet because as Professional Pet Foods (2013) explain, “excess ash will contribute excess minerals to the diet, potentially leading to health problems.” Method This method can be used for all types of food containing less than 50% fat, it allows for the determination of the mineral content of foodstuffs. In this process, the samples temperature is taken to 550°C to ensure all organic matter is...
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...Baghpat Crossing, MEERUT 250 005 UP, India 2 Department of Pharmaceutics, Laureate Institute of Pharmacy, KATHOG 177 101 The. Dehra, Dist. Kangra, HP, India Abstract: Piper betle leaves (Family: Piperaceae) have been used in Chinese and Indian folk medicine for centuries and recently been proposed to be used as a chemopreventive agent because of its anti-oxidant activity. In the present paper, Piper betle leaves were standardized for stomatal index, vein islet and vein termination numbers, palisade ratio, UV fluorescence and different ash values. The Piper betle leaves are earlier reported to possess anticancer potential. Hence, the aqueous extract of the leaves was subjected to cytotoxicity studies on Hep-2 cell line using MTT and SRB assays. The mean CTC50 was found to be 96.25 µg/mL, which proved the potent cytotoxicity and hence, the probable anticancer property of the selected extract. Key words: Betel Leaf Anticancer Phytochemical characterization and its leaves, with a strong pungent and aromatic flavour, are widely consumed as a mouth freshener [4]. The leaves are credited with wound healing, digestive and pancreatic lipase stimulant activities in the traditional medicine [5]. During our exploration of non-toxic and affordable herbal medicinal formulations, the PBL extract and its constituent phenolics were found to show impressive anticancer activities [6-8]. The deep green heart shaped leaves of betel vine are popularly known as Paan in India [9, 10]. MATERIALS AND...
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...have higher peroxidase activity levels because it was under more stress than the plant that was watered with water. Through the use of such techniques as tissue prints, dot-blot assays, electrophoretic analysis, and spectrophotometric assays, peroxidase activity levels were visualized and quantified. The results were as expected; the tissue from the plant that had been treated with Vitamin Water was more purple on the tissue prints and it was spectrophotometrically determined that the plant that had been treated with Vitamin water had a higher amount of peroxidase activity than the one that was treated with water. The results proved that the hypothesis was correct; stress on a plant, Vitamin Water in this case, causes higher levels of peroxidase activity. Introduction: Peroxidases are enzymes that use hydrogen peroxide as an electron acceptor to catalyze oxidative reactions. Peroxidase is believed to play a major role in synthesizing cell walls, preventing toxins and responding to stress. It is responsible for the purple color that is seen throughout the experiments. Peroxidase can be found in different locations in a plant in the form of isoenzymes. Many different experiments can be done to locate, visualize and quantify the activity of peroxidase enzymes. These include, tissue printing, dot-blot assays, electrophoresis, and spectrophotometry. This knowledge of what peroxidase does and how its activity levels can be detected was used to develop...
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...cancer [2]. Chemotherapy is restricted by both intrinsic and acquired cell resistance to drugs. [3] This has necessitated the use of natural products for the treatment of cancer. There are compelling evidences for experimental investigations on the efficacy of plant drugs against cancer [4]. It is known that there are links between the inflammatory and nociceptive, oxidative and cancer processes. The ability to inhibit any of the process will definitely lead to the inhibition of the others [5]. Based on the fact that M. calabura fruit possess antioxidant activity, the aim of the present study was to determine the in vitro anticancer activity of methanol extracts of the fruits of M. calabura against HeLa and HEp-2 cancer cell lines using MTT assay. Plant Material and Extraction Collection and authentication of fruits of M. calabura Linn were collected from the surrounding areas of Erode, Tamil Nadu state, India during 2008-2009 and the plant was identified and authenticated and deposited (voucher number: BSI/SC/5/23/09-10/Tech-132) at Tamil Nadu Agricultural...
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...The Brad assay * is one of the most common forms of measuring absorbance through a spectrometer * uses a a dye (coomassie Brilliant Blue G-250) that binds to proteins and is absorbed at a wavelength of 465- 598 nm * Fast, therefore can meaure a 96 plate in 5 minutes * Absorbance shift allows the measure the protein at the 598 nm wavelength by using the spectrometer, can be justified through the beer-lambert law * The standard curve can be used to estimate the concentration of an unknown protein in solution by * Taking in account their known absorbance levels * Use line equation from curve * The unknown protein conc. in a sample with a known absorbance level can be determined Bradford Vs. Lowry * Lowry * Uses alkaline conditions- reduction cu 2+ to Cu+ * Folin phenol reagent- yellow to blue * Peptide bonds for monocovalent copper and radicals of try and trp react with folin * Concentrations 0.1-1.0 ug/ml of protein * Can be interfered with easily * Slow 40-60 * Colour varies with proteins * Criticial timing with procedure * * Bradford * Based on absorbance shift from brilliant blue when bound to proteins * 465- 598 (when bound) * Acidic conditions * Binds tightly to protein, and inhibits binding sites (little inference) * Limitations * Strong basic buffers Detergents interfere with binding of the cosmic blue, causing...
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...4PY013 MOLECULAR BASIS OF LIFE PROTEIN ASSAY BY BIURET AND FOLIN METHODS Student Number and Name:1316740 Katerina Koupa | Name of Lab Instructor: Dr. J. Walton | Date of the experiment:06/02/2014 | Date of Report Submission:20/02/2014 | (Fig.1) Biuret Reagent Structure ABSTRACT: This report describes the use of the Biuret and Folin methods of protein assay, to verify the protein concentrations of two unknown solutions. To calculate the protein concentrations of the unknowns, nineteen samples were passed through a spectrophotometer which twelve of them had a known protein concentration and there absorbance levels were found. Then a calibration graph was plot that determines the concentration levels of the unknown samples. INTRODUCTION: One of the most typical procedures applied by lab scientist is the protein quantification. Finding protein concentrations in solutions is important in many ways. First of all detecting the levels of a protein in body fluids can result in identifying various diseases. Also protein verification is required for characterization and purification of enzymes. Because of the significance of protein assays laboratories perform this techniques on almost a daily basis. For this experimental procedure, the Biuret and Folin methods were used to determine the protein concentrations of two unknown samples. First the Biuret method (Fig.1) is based on the appearance of peptides bonds in proteins...
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...Essay - Free Essay Writing Samples Online Adwww.curriki.org/writing-essays 1. Get Samples for Teachers & Students * Support Child Education * Register With Us * Open Educational Resource ------------------------------------------------- Top of Form Bottom of Form Search Results 1. www.scribendi.com a short literary composition on a particular theme or subject, usually in prose and generally analytic, speculative, or interpretative. 2. anything resembling such a composition: a picture essay. Essay | Define Essay at Dictionary.com dictionary.reference.com/browse/essay More about Essay Feedback Essay - Wikipedia, the free encyclopedia en.wikipedia.org/?title=Essay Essays are generally scholarly pieces of writing giving the author's own argument, but the definition is vague, overlapping with those of an article, a pamphlet and a short story. Michel de Montaigne - Five paragraph essay - Application essay - Introduction Writing Tips: Essay Builder - WritingDEN www2.actden.com/writ_Den/tips/essay/index.htm Explores the parts and provides step-by-step directions for writing essays. Essay | Define Essay at Dictionary.com dictionary.reference.com/browse/essay a short literary composition on a particular theme or subject, usually in prose and generally analytic, speculative, or interpretative. 2. anything resembling such a composition: a picture essay. Images for essayReport images More images for essay Essay Structure | - Harvard Writing Center - Harvard...
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...that, we see how Leeland through his life, tries to find a successful career, but fails consistently. He moves various times from place to place, too seek occupation and good business. But it is hard when you’re a high school dropout, without a career. Leeland have to changes his job constantly, because of his lack of luck, and since he can’t get along white his bosses. He is never able to stay at one job or place for long, which lead to problems in the family. He has a hard time supporting his wife, and their children financially. Throughout the story the author, Annie Proulx manages to show us the miserable live of Leeland Lee, without even adding any emotion to her writing. Meaning that there is no point in the story, when we hear about how Leeland feels, about his miserable life. The writing is in typical Annie Proulx fashion, with short, fragmented sentences There is not a moment in the story, where the action takes a different turn, however we keep on hearing about...
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...GUIDELINES FOR WRITING LETTERS OF RECOMMENDATION Letters of recommendation are used to gather additional information about a candidate for employment, graduate study or other opportunity. It should give an overall picture of the candidate's: personal characteristics performance experience strengths capabilities professional promise Preferably, the person writing the letter of recommendation has been in a working or academic relationship with the applicant. It is recommended that the student provide you with a copy of their resume, along with the job description they are applying for. The letter should be about one page in length and generally consist of three parts: opening, body, and closing. Two questions to ask yourself BEFORE accepting a request to write a letter of recommendation 1. Can you speak positive about this individual? If you find yourself making up half truths, or having trouble finding positives, then you need to politely decline serving as a reference, or writing a reference letter. To avoid hurting feelings, simply state you feel you are not familiar enough with their background or work ethic to provide the best recommendation. 2. Can you speak for the applicant on the position they are applying for? If an individual asks you to be a reference, or to provide a reference letter and you do not feel you can provide a well rounded background reference, then you may want to consider either politely declining, or setting up an appointment with that individual...
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...An essay is a piece of writing which is often written from an author's personal[->0] point of view[->1]. Essays can consist of a number of elements, including: literary criticism[->2], political manifestos[->3], learned arguments[->4], observations of daily life, recollections, and reflections of the author. The definition of an essay is vague, overlapping with those of an article[->5] and a short story[->6]. Almost all modern essays are written in prose[->7], but works in verse[->8] have been dubbed essays (e.g. Alexander Pope[->9]'s An Essay on Criticism[->10] and An Essay on Man[->11]). While brevity usually defines an essay, voluminous works like John Locke[->12]'s An Essay Concerning Human Understanding[->13] and Thomas Malthus[->14]'s An Essay on the Principle of Population[->15] are counterexamples. In some countries (e.g., the United States and Canada), essays have become a major part of formal education[->16]. Secondary students are taught structured essay formats to improve their writing skills, and admission essays[->17] are often used by universities[->18] in selecting applicants and, in the humanities and social sciences, as a way of assessing the performance of students during final exams. The concept of an "essay" has been extended to other mediums beyond writing. A film essay is a movie that often incorporates documentary film making styles and which focuses more on the evolution of a theme or an idea. A photographic essay[->19] is an attempt to cover a topic...
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...Value: 15% of final grade Due: April 19, 2012 for Group 2. Paper must be submitted on the appropriate day, in class. Late submissions will receive a zero; no exceptions but for proven medical reasons. For future work to be graded this assignment must be handed in. Task (place a check in each box to verify that you have completed the step) You will submit a tentative introduction (ideally a re-worked version of the intro), as well as, the second third of the paper; o Depending on the format you have opted for (stacked or blended) this will consist of either the second discipline or the second discussion point (in which each discipline must be present) Ensure that you include a reference list of sources used in writing the second third of the paper. Your reference list should consist only of those sources used in the second third of the paper. At least 2-3 of your sources should be academic. Remember that your final paper will need a minimum of 10 sources of which at least 6 must be academic and one in French. You must also have a cover page which, at a minimum, has your name and a word count Your submission must have a minimum of 850 words (excluding cover page and reference list). More words is acceptable, fewer is not Include the original copies of your proposal and outline You must submit the notes from your sources A completed version of this checklist All of this must be in a folder ...
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