HYPOTHESIS 16 2 MATERIALS AND METHODS 17 2.1 HCV POSITIVE SERA SAMPLES 17 2.2 RNA EXTRACTION 17 2.3 CDNA SYNTHESIS 17 2.4 HCV PRIMER DESIGN AND USAGE 18 2.5 NESTED POLYMERASE CHAIN REACTION (NPCR) 21 2.5.1 REACTION AND CYCLING CONDITIONS 21 2.5.2 PCR PRODUCT PURIFICATION 22 2.6 AGAROSE GEL VISUALISATION 22 2.7 DNA SEQUENCING 22 2.8 DNA SEQUENCE AND PHYLOGENETIC ANALYSIS 23 2.9 KUNJIN VIRUS PLASMID 23 2.10 KUN PRIMER
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able to: | To achieve a distinction grade the evidence must show that the learner is able to: | P1 Compare and contrast the structure of various nucleic acids. | M1 Explain how genetic information can be stored in a sequence of nitrogenous bases in DNA. | D1 Explain the steps involved in biosynthesis of protein including the roles of
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The isolation and purification of genomic DNA requires three main processes which include, lysing of cells to release the DNA, purifying the solution by removing unwanted macromolecules, and precipitating the DNA from the solution. The process of isolation and purification requires several reagents. The Nuclei Lysis Solution contains lysozyme and EDTA. The lysozyme is an enzyme that degrades peptidoglycan, which is a rigid exoskeleton cell wall that protect the bacteria (3). Disrupting the peptidoglycan
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DNA, one of the most important molecules of all living organisms, holds the genetic imformation that determines the strucure of proteins of every individual organism. Being used in millions of organisms, those living and non-living, DNA can be formed in almost an infinite amount of combinations, making millions of unique traits. DNA has been used in the organisms living on the Earth since the beginning of life, about three or four billion years ago. The search for the chemical identitiy of genetic
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Gene Marker Identification Targeting Toll-Like Receptor 4 (TLR4), Breast Cancer 1 (BRCA1), and Adenosine Triphosphatase 1 Alpha 1 (ATP1A1) Genes: Assessing Their Association With Subclinical Mastitis Cases in Dairy Water Buffaloes, Bubalus bubalis Thesis Proposal Cyndi Candelaria Biendima Patricia Malapit Cabatit Submitted to the Department of Biology College of Arts and Sciences University of the Philippines Manila Padre Faura, Ermita, Manila In partial fulfilment of the requirements
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illnesses and deficiency (The quality or state of being defective or of lacking some necessary quality or element). A well known example of this is the golden rice, the golden rice is a new transformed rice which has been made by genetically modifying the DNA and incorporating the genes of two different rice to make a rice which has the presence of Beta carotene which is the source of vitamin A. This rice has been known to help in the aid of vitamin deficiency. In South and south east Asia vitamin A deficiency
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HYPOTHESIS 16 2 MATERIALS AND METHODS 17 2.1 HCV POSITIVE SERA SAMPLES 17 2.2 RNA EXTRACTION 17 2.3 CDNA SYNTHESIS 17 2.4 HCV PRIMER DESIGN AND USAGE 18 2.5 NESTED POLYMERASE CHAIN REACTION (NPCR) 21 2.5.1 REACTION AND CYCLING CONDITIONS 21 2.5.2 PCR PRODUCT PURIFICATION 22 2.6 AGAROSE GEL VISUALISATION 22 2.7 DNA SEQUENCING 22 2.8 DNA SEQUENCE AND PHYLOGENETIC ANALYSIS 23 2.9 KUNJIN VIRUS PLASMID 23 2.10 KUN PRIMER
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Abstract: Neuropathic pain is chronically increased pain and heat sensitivity resulting from damage to the central or peripheral nervous system. Neuropathic pain is constant, and difficult to treat, because the pain persists even after the injury has healed. Current drug treatments are ineffective, have short durations, and have unpleasant side effects. The SIP-30 gene is a candidate gene for involvement in neuropathic pain. When neuropathic pain is present, SIP-30 levels rise indicating
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material. If the plant’s DNA is isolated and experience a series of polymerase chain reaction (PCR), it is possible to identify the existence of 35s promoter by looking at a band around 162bp in an electrophoresis gel.
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International Journal of Biological Sciences Impact Factor ISSN 1449-2288 News feeds of IJBS published articles Manuscript Status/Login Contact 6 May 2015 Home Index & Ranking Current Issue Archive Cover Images Editorial Board Author Info Submission Special Issues Contact Journal of Genomics now in PubMed/PubMed Central. Submit manuscript... Top Introduction Materials and Methods Results Discussion Supplementary
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