1. What is the purpose of flaming the loop before and after use? This process sterilizes the inoculating loop before the experiment. By passing the inoculating loop through the warmest part of the flame (the tip of the inner blue flame), the heat kills any microbes and prevents contaminating microorganisms from interfering with the integrity and accuracy and contaminating your experiment. This process is known as flame sterilization. Flaming the loop after the experiment once again kills any contaminating
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OSI Seven Layer Model The Open System Interconnection model (OSI) defines internetworking in terms of a vertical stack of seven layers. The upper layers of the OSI model represent software that implements network services like encryption and connection management. The lower layers of the OSI model implement more primitive, hardware-oriented functions like routing, addressing, and flow control(Mitchell, 2011).The seven layers of the OSI model are - * Physical * DataLink * Network
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Bacteria are very small organism, called microorganisms that are present in various types of environment and can only be seen using a microscope. They are classified in different ways depending on their cell wall, shape and genetic, they are called prokaryotic cells because they do not contain a nucleus and their DNA is spread throughout its cytoplasm. Bacteria can be found in the ocean, soil, inside the human gut, air, water and most of the materials we touch. There are some types of bacteria that
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were added to a thermocycler. The thermocycler’s role is to provide strict control over the reaction temperature and duration of exposure to each distinct temperature, this ensures the amplification process. We also poured the prepared LB agar into petri dishes to be stored in the fridge as well as leaving our LB media in its container at our lab benches until further use for the upcoming weeks in lab. For this past week in lab transformation was carried out. The first thing we did was to
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The results from the experiment does not support my hypothesis about the role of the FIS binding site in the rR promoter. I believed that if an E. coli strain did not have an rR promoter sequence like Strain B, or if the FIS binding sequence was completed deleted like Strain C, it will be more difficult for transcription to begin. My hypothesis was that Strain A would be the most efficient because it had a fully intact FIS binding site. Clearly, this is not the case because from the results I collected
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The effect of glucose on the growth of mung beans as measured in percentages of glucose mixed in water Annie Spendrup I. Purpose The purpose of this lab was to examine the effect of a glucose-water mixture on the growth and development of mung beans. Different percentages of glucose (0%, 1%, 2%, 3%, and 4%) in 40 millilitres of distilled water were used to water the mung beans. The hypothesis is that seeds watered with a glucose water solution will grow faster and taller than the ones
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CHAPTER 1 INTRODUCTION 1.1 General The micro-emulsion inks have a wide range of applications in industry. The ink used earlier for printing purposes contributes to pollution in environment. The pollution is by means of emission of volatile organic compounds (VOCs) which are present in the form of an aliphatic solvent. Emission of VOCs from these solvents and their discharge in waste streams are quantifiable. For example a typical medium size cold – set web plant uses 40,000 kg per year
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This is due to the small size of the Artemia nauplii, and it would have been easier to observe their movement in smaller petri dishes. The coloured filters used to create different wavelengths of light may have caused the light intensity from the torch to have decreased, so the intensity of light may have varied for each wavelength of light. As different torches were used
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I. TITLE: GENE TRANSFER OF ANTIBIOTIC RESISTANCE AND BIOLUMINESCENCE IN ESCHERICHIA COLI II. INTRODUCTION A. Background on antibiotics 1. History of antibiotics a. Fleming’s discovery of penicillin and its early significance -antibiotic inhibition of protein synthesis, cell wall synthesis, and other cellular processes b. Antibiotic overuse led to bacterial resistance Background on the transfer of resistance genes from one bacterium to another 1. Location of
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are the steps for immobilizing the flies. Hold the vial containing the flies at an angle and place it in a refrigerator for several minutes. When the flies are immobilized, place them into a small plastic Petri dish. 3. Use the dissecting microscope to view the flies. Make sure to top the petri dish on when viewing the flies. 4. You can easily distinguish male flies from females by looking for the following characteristics: males are usually smaller than the females, males have dark blunt abdomens
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