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Glioblastoma Personal Statement

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Biographical Information: It’s difficult to remember a time when science wasn’t an integral part to my life. In seventh grade my fascination with science commenced when cells were introduced to me. Microscopic parts of the living world that worked together to form who I was, yet could exist individually as its own self was startlingly foreign and I wanted to learn everything I could about it. I was able to cultivate this desire of discovery when I was accepted to Biotechnology High School, the public magnet school with a focus on applications of biology. It was there that I found my love of research and was able to conduct my own project annually from genetically engineering bacteria to degrade methane to testing the effect of a medicinal mushroom …show more content…
Here, a methodology is described in order to combat the growth of glioblastoma, while simultaneously re-activating the immune system to recognize and effectively terminate glioblastoma cells. Epidermal growth factor receptor (EGFR) is a gene for cell receptors that is overexpressed in glioblastoma, contributing to the proliferation of the cancer. Gene therapy, such as pre-trans splicing has been demonstrated to be an effective strategy in blocking the expression of EGFR. A pre-trans splicing RNA molecule (PTRM) was designed by reviewing the literature. Additionally, interleukin 13 receptor alpha variant 2 (IL13Rα2) has become an effective target for immunotherapy due to being selectively expressed in glioblastoma and the strong immune responses of cytotoxic T-lymphocytes (CTLs) it elicits. Cloning a portion of IL13Rα2 as an immunogen into the multiple cloning site of the PTRM would lead to greater IL13Rα2 expression and therefore, greater immunogenic potential of the tumor microenvironment. It is expected that delivery of hybrid PTM and immunogen in an adeno-associated virus plasmid vector would lead to synergistically inhibiting EGFR expression with the potential to reactivate the CTL

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