...Microbiology Overview Interpretation of preliminary microbiology data Gram-positive cocci Aerobic In clusters ● Coagulase (+): Staphylococcus aureus ● Coagulase (-): Staphylococcus lugdunensis and other coagulasenegative staphylococci In pairs/chains ● Optochin sensitive: Streptococcus pneumoniae ● Alpha-hemolytic: Viridans group Streptococcus, Enterococcus ● Beta-hemolytic: ○ Group A Strep (Streptococcus pyogenes) ○ Group B Strep (Streptococcus agalactiae) ○ Group C, D, G Strep Anaerobic: Peptostreptococcus spp. and many others Gram-positive rods Aerobic ● Large: Bacillus spp ● Cocco-bacillus: Listeria monocytogenes, Lactobacillus spp ● Small, pleomorphic: Corynebacterium spp ● Branching filaments: Nocardia spp, Streptomyces spp Gram-negative cocci Aerobic ● Diplococcus: Neisseria meningitidis, N. gonorrhoeae, Moraxella catarrhalis ● Cocco-bacillus: Haemophilus influenzae, Acinetobacter Anaerobic: Veillonella spp. Gram-negative rods Aerobic Lactose fermenting (Lactose positive): ● Enterobacter spp, Escherichia coli, Klebsiella spp ● Citrobacter spp*, Serratia spp* Non lactose-fermenting (Lactose negative): ● Oxidase (-): Acinetobacter spp, Burkholderia spp, E. coli, Proteus spp, Salmonella spp, Shigella spp, Serratia spp*, Stenotrophomonas maltophilia ● Oxidase (+): P. aeruginosa, Aeromonas spp. Anaerobic ● Large: Clostridium spp Anaerobic: Bacteroides spp, Fusobacterium spp, Prevotella spp. ● Small, pleomorphic: P. acnes, Actinomyces spp *Serratia and Citrobacter spp...
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...flu) pandemic. The estimated transmissibility of the current virus is also not far from the norm. Depending on the methodology used, the calculated basic reproduction number (R0; the number of secondary infections produced by a single infected individual) is 1.2-1.6. This number is similar to that seen with seasonal influenza, while comparable estimates of R0 for the 1918, 1957, and 1968 pandemics ranged from 1.4-2.0. The WHO, however, suggests that there may be a much higher secondary attack rate. ■ COMMENTARY 2. 3. 4. 5. Government Pandemic Influenza Site: http://www.PandemicFlu.gov/ U.S. Government Pandemic Influenza Site: http://www.PandemicFlu.gov/ World Health Organization http://www.who.int/csr/disease/swineflu/en/index.html Human/Swine A/H1N1 Influenza Origins and Evolution (Wiki) http://tree.bio.ed.ac.uk/groups/influenza/ http://www.thelancet.com/H1N1-flu The recognition that the initial perception of a high mortality rate was apparently spurious has led to some relaxation of concern. This may, however, be premature. While we have completed the usual influenza season in the Northern Hemisphere, the season is just beginning in the southern latitudes. Furthermore, previous pandemics have come in waves, with the second or third wave sometimes being associated with more severe disease than the original portion of the epidemic. While this was not true of the 1968 pandemic, it was true in 1957-1959 and, especially, in 1918. In fact, concern has been raised about...
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...BIO212 Microbiology Exam 3 Essay Portion Directions: Both the multiple choice portion and the essay portion of the examination will be available on Wednesday of Module 8 at 12:01 AM ET. The essay portion will close on Sunday at 11:55 PM ET. Choose 7 of the following 10 questions listed below. Your answer for each question should be a minimum of 150 words (half page). Please number your answers and print the number of the question you are answering above your answers. Identify subsections accordingly. We suggest you write or print out the essay questions for handy reference as you develop your answers. 1) Blood isolated from an emergency room patient contains antibodies against the measles virus. Please explain whether or not the patient should be quarantined immediately because they are infected with the measles virus and why? What are the other plausible explanations for anti-measles antibodies in the patient’s blood? 2) Describe how the clonal expansion theory explains the more rapid production of antigen specific antibodies, Helper T-cells and Cytotoxic T-cells, during a secondary infection. 3) Positive diagnosis of AIDS is made when a patient has antibodies against the human immunodeficiency virus (HIV). Please explain why the patient has an immune deficiency if he or she is making antibodies? 4) Characterize the differences and similarities between innate and adaptive immunity in form and function. 5) What is the effect of discharging primary-treated sewage on the...
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...Microbiology Homework Assignment – Microbial/Bacteria/Viral Diseases Directions: Answer each question. Be sure to answer the ENTIRE question. See numbers in parentheses ( ) for point values. You must handwrite your answers in the space provided. In addition to answering the questions, you will be graded 5 points based on the neatness of your submitted work (including your handwriting). If I cannot read the answer, you will get 0 points for the question. If you use another source (website, journal article, etc.) to help you answer a question, you must cite it. 1. Name and describe the 3 types of symbiosis. (6) * Mutualism- a relationship between two species of organisms in which both benefit from the association. * Commensalism- a type of relationship between two species of a plant, animal, fungus, etc., in which one lives with, on, or in another without damage to either. * Parasitism- a relation between organisms in which one lives as a parasite on another. 2. Pick one of the types of symbiosis and find an example and describe it (using your textbook or the internet). (2) * Example of Commensalism: Remarkable cases occur of commensalism between certain crabs and sea-anemones, and they betoken much intelligence. 3. You want to prove that the Mycoplasma bacterium is the cause of tuberculosis. Describe how you would prove this using Koch’s postulates. (4) * The microorganism or other pathogen must be present in all cases of disease ...
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...the international health community is Hepatitis B. Hepatitis B is viral condition that injures the liver due to the attempt to eliminate the virus, leading to progressive liver disease and cirrhosis (Hepatitis B (HBV, Hep B), 2014). The incidence of cirrhosis developing in people with chronic HBV is 8-20% (National Institute for Health and Care Excellence, 2013; European Association for the Study of the Liver, 2012); the annual incidence of HBV-related liver cancer, hepatocellular carcinoma, is 2-5% of people with cirrhosis (EASL, 2012). A person infected with Hepatitis B can transmit the virus to another person who hasn’t been affected by Hepatitis B in several ways. People can become infected with the virus when they come into contact with contaminated blood, semen and when other body fluids mix with the uninfected person’s body fluids. Examples of transmission occur from sexual contact, blood transfusion before the year 1992, sharing of needles or syringes, and from mother to baby at birth. Hepatitis B can be an acute, or short-term, illness for some people but for others, it can often lead to a lifelong chronic infection. The risk for chronic infection is related to a person’s age at the time of infection: “Approximately 90% of infected infants become chronically infected due to being birthed from their mother who carries the virus, compared with 2%–6% of adults. Chronic Hepatitis B can lead to serious health issues, like cirrhosis or liver cancer” (National Institute for...
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...NINTH EDITION Burton’s MICROBIOLOGY FOR THE HEALTH SCIENCES Paul G. Engelkirk, PhD, MT(ASCP), SM(AAM) Biomedical Educational Services (Biomed Ed) Belton, Texas Adjunct Faculty, Biology Department Temple College, Temple, TX Janet Duben-Engelkirk, EdD, MT(ASCP) Biomedical Educational Services (Biomed Ed) Belton, Texas Adjunct Faculty, Biotechnology Department Temple College, Temple, TX Acquisitions Editor: David B. Troy Product Manager: John Larkin Managing Editor: Laura S. Horowitz, Hearthside Publishing Services Marketing Manager: Allison Powell Designer: Steve Druding Compositor: Maryland Composition/Absolute Service Inc. Ninth Edition Copyright © 2011 Lippincott Williams & Wilkins, a Wolters Kluwer business © 2007 Lippincott Williams & Wilkins, © 2004 Lippincott Williams & Wilkins, © 2000 Lippincott Williams & Wilkins, © 1996 Lippincott-Raven, © 1992, 1988, 1983, 1979 JB Lippincott Co. 351 West Camden Street Baltimore, MD 21201 Printed in the People’s Republic of China All rights reserved. This book is protected by copyright. No part of this book may be reproduced or transmitted in any form or by any means, including as photocopies or scanned-in or other electronic copies, or utilized by any information storage and retrieval system without written permission from the copyright owner, except for brief quotations embodied in critical articles and reviews. Materials appearing in this book prepared by individuals as part of their official duties as U.S. government employees...
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...Section A: Basic Microbiology 1 SCOPE AND HISTORICAL DEVELOPMENTS IN MICROBIOLOGY “Science contributes to our culture in many ways, as a creative intellectual activity in its own right, as a light which has served to illuminate man’s place in the uni-verse, and as the source of understanding of man’s own nature” —John F. Kennedy (1917–63) The President of America The bacterium Escherichia coli INTRODUCTION AND SCOPE MICROBIOLOGY is a specialized area of biology (Gr. bios-life+ logos-to study) that concerns with the study of microbes ordinarily too small to be seen without magnification. Microorganisms are microscopic (Gr. mikros-small+ scopein-to see) and independently living cells that, like humans, live in communities. Microorganisms include a large and diverse group of microscopic organisms that exist as single cell or cell clusters (e.g., bacteria, archaea, fungi, algae, protozoa and helminths) and the viruses, which are microscopic but not cellular. While bacteria and archaea are classed as prokaryotes (Gr. pro-before+ karyon-nucleus) the fungi, algae, protozoa and helminths are eukaryotes (Gr. eu-true or good+ karyon-nucleus). Microorganisms are present everywhere on earth, which includes humans, animals, plants and other living creatures, soil,water and atmosphere. Microorganisms are relevant to all of our lives in a multitude of ways. Sometimes, the influence of microorganisms on human life is beneficial, whereas at other times, it is detrimental. For example...
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...Unit 1 An overview of Microbiology 1. What prevented the science of microbiolgy from developing before the era of van Leeuwenhoek? 2. How did Pasteur's swan neck flask experiment show that the concept of spontaneous generation was invalid? 3. If the Chickungunya virus epidemic had first started in Jamaica, how would you identify the cause of the disease? 4. What is a pure culture? How is this important in microbiology? 5. How would you convince a friend that microorganisms are not just agents of disease but make significant contributions to their lives? 6. Describe 5 ways humans exploit microorganisms for their benefit. 7. What factors might cause some older diseases to show an increase in number of cases. UNIT 2 Prokaryotic and Eukaryotic Cell Biology 1. Describe the structure of the cell membrane and describe its components. 2. Explain the following terms with the use of diagrams: a) isotonic b) hypertonic c) hypotonic 3. Compare diffusion, facilitated diffusion, active transport and group translocation. UNIT 3 Main Groups of Microorganisms 1. Discuss reproduction of algae and fungi. 2. Describe how protozoa are classified. Give the name of member of each group and a disease caused by that organism. 3. Outline how viruses reproduce UNIT 4 Bacteria 1. Outline the process of endospore formation 2. Draw and label the structure of flagella found in gram negative organisms 3. State the various...
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...UNIVERSITY OF CAPE COAST SCHOOL OF AGRICULTURAL ASSIGNMENT COURSE TITLE: AGRICULTURAL MICROBIOLOGY COURSE CODE: AGN201N INDEX NUMBER: AG/AGR/11/0036 QUESTION: 1. Examples of pathogenic bacteria and their corresponding diseases. 2. Examples of useful bacteria and their importance 30TH August, 2012 Bacteria are living things that belong to a group all by themselves. They are small, single cell organisms called prokaryotes that do not contain a nucleus and are usually found in very large groups because they can quickly multiply. There are many different kinds of bacteria that are all separated into different types and groups, each group having its very own unique qualities that sets it apart from all the rest. A pathogen is a microorganism – in the widest sense, such as a virus, bacterium, prion, or fungus – that causes disease in its host. The host may be an animal (including humans), a plant, or even another microorganism. Pathogenic bacteria are bacteria that cause bacterial infection. Below is a table of some pathogenic bacteria and their corresponding diseases. PATHOGENIC BACTERIA | PATHOGENIC DISEASE | Clostridium perfringens | * Gas gangrene * Acute food poisoning * Anaerobic cellulitis | Streptococcus pyogenes | * Streptococcal pharyngitis * Scarlet fever * Rheumatic fever * Impetigo and...
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...International Committee on onomy of Viruses. "The Universal Virus Database, version 4: Influenza A". http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/00.046.0.01.htm. [dead link] 1. ^ a b c d e f "Swine influenza". The Merck Veterinary Manual. 2008. ISBN 1442167424. http://www.merckvetmanual.com/mvm/index.jsp?cfile=htm/bc/121407.htm. Retrieved April 30, 2009. 2. ^ [1][dead link] 3. ^ Heinen PP (15 September 2003). "Swine influenza: a zoonosis". Veterinary Sciences Tomorrow. ISSN 1569-0830. http://www.vetscite.org/publish/articles/000041/print.html. "Influenza B and C viruses are almost exclusively isolated from man, although influenza C virus has also been isolated from pigs and influenza B has recently been isolated from seals." 4. ^ Bouvier NM, Palese P (September 2008). "THE BIOLOGY OF INFLUENZA VIRUSES". Vaccine 26 Suppl 4 (Suppl 4): D49–53. doi:10.1016/j.vaccine.2008.07.039. PMC 3074182. PMID 19230160. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=3074182. 5. ^ Kimura H, Abiko C, Peng G et al. (April 1997). "Interspecies transmission of influenza C virus between humans and pigs". Virus Research 48 (1): 71–9. doi:10.1016/S0168-1702(96)01427-X. PMID 9140195. 6. ^ a b Matsuzaki Y, Sugawara K, Mizuta K et al. (February 2002). "Antigenic and Genetic Characterization of Influenza C Viruses Which Caused Two Outbreaks in Yamagata City, Japan, in 1996 and 1998". Journal of Clinical Microbiology 40 (2): 422–9. doi:10.1128/JCM.40.2.422-429.2002. PMC...
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...Microbiology Exam II Fall 2001 Name: _____________________________________________ Multiple Choice: Circle the most correct answer for each of the following questions. (2 points each) 1. In a DNA molecule, which base would you expect to find paired with cytosine? a. guanine b. uracil c. adenine d. thymine e. cytosine 2. DNA serves as the template to initiate protein synthesis. What is directly formed from this template? a. protein b. mRNA c. tRNA d. ATP e. Amino acids 3. What does ligase do during replication of DNA? a. Makes copies of mRNA from DNA b. Removes damaged sections of DNA c. Joins together mRNA d. Joins together DNA segments e. Digests mRNA when it is no longer needed 4. Which is mismatched? a. Replication = formation of 2 identical DNA molecules b. Transcription = mRNA synthesized from a DNA template c. Translation = tRNA transfers amino acids to the ribosomes based on mRNA codons d. Nonsense codons = terminator codons e. Anticodon = sequence of 3 nitrogenous bases on the gene 5. A red pigment produced by a bacterial species is an example of a(n)? a. Phenotype b. Genotype c. Operon d. Promoter e. Inducible enzyme 6. Which of the following best describes a plasmid? a. A gene within the...
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...SeAnna Brantley Microbiology Case Studies 1.1-1.8 Case 1.1 1. The fertility specialist recommended the rubella vaccine because if the rubella virus is contracted early in pregnancy there is an increased risk of miscarriage or birth defects such as deafness, cataracts, mental retardation, heart defects, liver and spleen damage. There are contraindications related to conceiving within four weeks of receiving the rubella vaccination because it is a “live” vaccine and could still cause complications. 2. In the United States it is recommended that children receive the first dose of the MMR (measles, mumps rubella) vaccination between 12-15 months (not earlier) and then to receive a second dose around 4-6 years of age. 3. The client’s description does not correlate with the rubella virus. Symptoms of the rubella infection typically only last 3 days and are often referred to as the “3 day measles”. 4. In order to check the immune status of the client the test would be checking for IgG antibodies. The absence of IgG rubella antibodies indicates that the person has likely not been exposed to the rubella virus or vaccine. IgG are also the only antibodies that can cross the placenta to protect the fetus. 5. To test for a current rubella infection using only the IgG antibody test the physician should order the initial IgG for a baseline and then perform the test again in 2-3 weeks to check if there was a significant increase (titer) present. An increase would indicate recent or current...
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...M2D1: Microscopy and Differential Staining 1. What are the advantages and disadvantages of the different types of light and electron microscopes discussed in Chapter 3 that are used to study microorganisms? Focus your response in terms of the following parameters: o Range of magnification o Resolving ability o Sample preparation o Possible states of sample (e.g. whole organism, part of, living, non-living, etc Compound Light microscopes magnification is 2000X. Resolution of about 0.2μm. Can only see very small specimens and specimens are stained. Darkfield – used to study live microorganisms that cannot be stained or staining distorts the image or they are invisible using the normal light microscope. Phase-Contrast – in living microorganisms, this scope allows you to see detailed internal structures, plus you do not have to fix or stain the microbes. Differential Interference Contrast (DIC) – instead of one beam of light, 2 beams are used. Image looks almost 3-dimensional and is brightly colored. Fluorescence – used mainly as a diagnostic technique. Stained with fluorochromes and viewed with an ultraviolent light. Confocal – makes 3-dimensional images using a computer. Able to see entire cells and their components. Two-Photon – living cells can be seen up to 1mm (1000um) deep in tissues. Can also track, in real time, the activity of cells. Scanning Acoustic – living cells that are attached to cancer cells, artery plaque and biofilms can be seen through...
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...testing must involve the definition of each and by this will help to narrow the field of the type of particular microbe involved. The first is of the genera Bacillus, Gram-positive and rod-shaped bacteria, has two separate meanings, first, a bacterial shape, second, when capitalized and italicized refers to a specific genus, each one form long twisted chains of cells. The second microbe involved is Escherichia, Gram-negative, rod shaped, bacterium that is part of our normal lower GI flora. In the incident of illness, there can be relation of this particular bacterium from food contamination. Lastly, Mycoplasma, smallest type of prokaryotic bacterium, thought to be related to Archaea, each lack a cell wall and was initially mistaken for a virus due to its size. (Tortora, 2014) The formation of colonies are related to odd shaped fried eggs, this being similar to fungal type...
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...domestication of animals, cultivation of plants, and "improvements" to these through breeding programs that employ artificial selection and hybridization. Modern usage also includes genetic engineering as well as cell and tissue culture technologies. Biotechnology is defined by the American Chemical Society as the application of biological organisms, systems, or processes by various industries to learning about the science of life and the improvement of the value of materials and organisms such as pharmaceuticals, crops, and livestock.[4] In other words, biotechnology can be defined as the mere application of technical advances in life science to develop commercial products. Biotechnology also writes on the pure biological sciences (genetics, microbiology, animal cell...
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