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Propanol And Haemolysis Lab Report

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The average hemolysis time for Thiourea and Triethylene Glycol appeared to be the highest out of all eight of the molecules with Triethylene Glycol at the top (figure 1). Diethylene Glycol and Ethylene Glycol came as the third and fourth highest (figure 1). Urea was the fifth highest in hemolysis time (figure 1). Lastly, Methanol, Ethanol, and Propanol, with their similar heights, had the shortest hemolysis time (figure 1).
Referring to the conventional criteria, the difference between the average hemolysis time of erythrocytes in methanol and ethanol was not statistically significant (t=0.2652, df=8, P=0.7976).
Referring to the conventional criteria, the difference between the average hemolysis time of erythrocytes in methanol and propanol …show more content…
The difference in average hemolysis time of urea and ethanol shown by Figure 1 illustrated the relationship between lipid/water partition coefficient of urea and ethanol. It illustrated that, with similar molar volumes, the greater the lipid/water coefficient, and the shorter time of hemolysis. This supported the hypothesis in which physical properties such as lipid/ water coefficient affect membrane permeability. Similarly, thiourea and propanol also illustrated the same relationship with similar volumes and different lipid/ water coefficient. Propanol, with the greater lipid/water partition coefficient, had a shorter hemolysis time. On the contrary, the lipid/partition coefficient of urea was similar to the lipid/partition coefficient of ethylene glycol, but the molar volume of urea was smaller than the molar volume of ethylene glycol. The average hemolysis time of urea was shorter than the average hemolysis time of ethylene glycol. This illustrated the relationship between molar volume and the rate of hemolysis, which supported the hypothesis that the molar volume …show more content…
2009. t-test calculator. Website: http://www.graphpad.com/quickcalcs/ttest1.cfm . February 9, 2015
Spilios K(ed).2015. Principles of Biology II. Hayden-McNeil Publishing, Plymouth, MI. Module 3, pp 41-58.
BI 108 Section E9. 2015. Principles of Biology II. Biology Department, Boston University, Boston, MA.
Sha’afi, Gary-Bobo, and Solomon 1971. Permeability of Red Cell Membranes to Small Hydrophilic and Lipophilic Solutes. The Journal of Cell Biology Vol. 58 pp238- 258.
Eckert, R., D. Randall, G. Augustine. 1988. Permeability and transport in Animal physiology (Third edition). W. H. Freeman, New York.
Oldendorf 1974. Lipid Solubility and Drug Penetration of the Blood Brain Barrier. The Society for Experimental Biology and Medicine. Vol.147

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