INTRODUCTION:
Toxicity can affect human, animals and plants. A toxic is something poisonous to a living organism. In order to determine how this will affect a living organism, an experiment should be conducted.
In this experiment it uses Fabric softener. Downy Fabric softener is a household chemical that is full of nitrogen, constantly exposed to the environment, and also claims to be biodegradable. Nitrogen is a limiting nutrient in plants and therefore the aid of nitrogen should effect growth. Early active ingredient of fabric softener is dimethyl ammonium chloride (DHTDMAC), which belongs to the materials known as quaternary ammonium compounds, or quats (Fig. 1). Fabric Softener was observed to be toxic to the plants by inhibited production. Each day for one week, the plants effects would be recorded and the designated amounts of concentrations were added. I chose concentrations 1ml, 5ml and 9ml because it was believed that fabric softeners have a strong agent of chemicals. There were studies also conducted that showed how fabric softener affects the plants.
I preferred this kind of test using fabric softener because it is very interesting, since fabric softener is commonly used by people in the laundry works.

Fig. 1 Dimethyl Ammonium Chloride (DHTDMAC). This kind of ingredients is useful because part of the molecule has positive charge that attracts and binds it to negatively charged fabric fibers.

HYPOTHESIS: The higher the concentration, the more it inhibits the growth of the mungbean plants.

OBJECTIVES:
The effects of different amounts of concentrations of Downy fabric softener will be tested on Mungbeans to determine whether or not this certain fabric softener is affecting the plant.

MATERIALS:
Mungbean plant (120 pcs) Tissue
Plant containers (12 pcs) Measuring tools
100 ml of Distilled water (3 pcs) Notes
Fabric Softener (Downy)

METHODOLOGY: 1. Collect and soaked mungbeans, for a couple of minutes. Those will descend will be planted, those ascends will not. 2. Prepare the container with water and tissue. 3. Place each 10 mungbeans into a container. (Repeat for 12 times) 4. Mix the different desired amount of fabric softener to a 100 mL distilled water. 5. Segregate the container with mungbeans for: 3 controlled; 3 for first concentration (1ml); 3 for the second concentration (5ml) and 3 for third concentration (9ml). 6. Designate each different concentration to different areas in a plot. Record it. 7. Start adding the different concentration to the designated containers. 8. Repeat it at the same time everyday for five days. Observe changes and record.
RESULTS:

Day 1

Controlled 1ml 5ml 9ml

Day 2

Controlled 1ml 5ml 9ml

Day 3

Controlled 1ml 5ml 9ml

Day 4

Controlled 1ml 5ml 9ml

Day 5

Controlled 1ml 5ml 9ml
INDEX

Length of Mung beans | | Controlled | T1 | T2 | T3 | Day 1 | 0.5 | 0.5 | 0 | 0 | Day 2 | 2 | 1 | 1 | 0.75 | Day 3 | 3 | 1.75 | 1.5 | 1 | Day 4 | 4.5 | 3 | 2 | 1.25 | Day 5 | 8 | 6.58 | 4 | 2 |

| Number of Mung beans | Controlled | 10 | T1 (1mL) | 10 | T2 (5mL) | 8 | T3 (9mL) | 6 |

DISCUSSION: Mungbean plants with different trial and replicate number were distributed from different area of the given plot. From the 12 experiments, each 6 was divided into 3 sets. Set 1 was placed where there is abundant in sunlight, set 2 was placed on the sunlit areas and set 3 was placed where there is no much sunlight.
It represents every day’s differences of the mungbean plants. It sums up the data of the experiment that the height and number of mungbean plants that grew varies from the concentrations. In the controlled data, plants were normally growing, and grew faster. But those with concentration of the toxicant, it lessen the number of growth of the plant. There’s a big difference between the controlled plants to those plant with toxicant. It explained the decreasing level with higher concentrations.
It showed the lethal dose 50 (LD50), which causes inhibition almost on the sample group of plant. In other means, the higher its concentration the lower the chance of the mungbean plants to grow and testifies that the hypothesis is accepted.

CONCLUSION:
Based on the data obtained, Downy Fabric softener in 9ml concentrations can inhibit the normal growth of the mungbeans. The chemicals in the fabric softener break down into and ultimately dehydrate the plant and if the chemicals do enter the cells of the plant, the plant becomes overcome with toxins from the fabric softener.

RECOMMENDATIONS: I would recommend to the future experiments that they should try it with soil, since not all plants can grow through tissues. Soils have different factors that could also affect the toxicant and the plant, which is interesting to discover. The number of days should be extended to 7 days or a week to show thorough results. The 9mL concentrations are not that too much. A little higher concentration will do, but around 15 mL concentrations could kill the plant.