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Human Amniotic Cell Sheet

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I hypothesize if human amniotic membrane (AM) is placed on top of the basal side of a cell sheet before being transplanted to the heart, then the cell sheet will adhere to the heart more quickly because of AM’s biocompatible properties. The human amniotic membrane will be collected from willing donors after birth. The AM will be washed with solutions before removing the epithelial cells from the membrane. The cells with then be put into a culture to grow before being placed on top of the three layered cell sheet. I will then begin creating a multi layered cell sheet with mesenchymal stem cells, which are stem cells found in the bone marrow that can distinguish into myoblasts (muscle cells). The bone marrow will be taken from the hip bones of

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