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Strain A Case Study

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The results from the experiment does not support my hypothesis about the role of the FIS binding site in the rR promoter. I believed that if an E. coli strain did not have an rR promoter sequence like Strain B, or if the FIS binding sequence was completed deleted like Strain C, it will be more difficult for transcription to begin. My hypothesis was that Strain A would be the most efficient because it had a fully intact FIS binding site. Clearly, this is not the case because from the results I collected, no strain had faster gene expression after ONPG was added to each strain of E. coli. I can only conclude that some error occurred somewhere within the experiment because a logical conclusion would be that the unmutated DNA promoter should have had a stronger gene expression. …show more content…
From my results, the strains of E. coli that had a FIS binding site resulted in absorbance numbers that were fairly similar to the strains of E. coli that did not have a FIS binding site. This similarity reveals that the FIS binding site does not have a large effect on transcription of the E. coli strains used within the experiment.

This color change report reveals that the beta galactosidase enzyme can reveal the rate at which cells are being broken down. This indicates the level of lacZ transcription because the more yellow color that appears equates to the amount of cells being broken down. This measurement can provide a reasonable estimate of promoter activity in the bacterial strains given because the darker the yellow color equates to more enzyme activity. A promoter sequence that is fully intact should result in a darker concentration than a mutated or completely missing promoter

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