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Warm Autoimmune Disorder Case Studies

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A pretransfusion sample is received in the laboratory from a 60 year old Caucasian female admitted for anemia. Patient history reveals a diagnosis of systemic lupus erythematosus (SLE) and no transfusions since receiving two units of blood two years earlier. Laboratory findings are: Hgb = 6.9 g/dL Hct = 21% Retic = 7% Total Bilirubin 2.2 mg/dL LDH = 450 U/L
Expected Results ABO/Rh = O Pos DAT = Positive (IgG only) Antibody Screen = Positive with all cells tested (2-3+ IAGT) Antibody identification panel = Positive with all cells tested (2-3+ IAGT) Autologous control = Positive (2-3+ IAGT)
Warm Autoimmune Hemolytic Anemia (WAIHA) is the most cause of the positive DAT and autologous control.
Warm antibody hemolytic anemia is an autoimmune disorder characterized by the premature destruction of healthy red blood cells by autoantibodies. Autoimmune diseases occur when the body's natural defenses against foreign organisms examples …show more content…
The warm autoantibody is coating the patient’s red blood cells (positive DAT) and is also present in the patient’s serum (antibody screen/antibody ID reactions).

Warm Autoimmune Hemolytic Anemia includes anemia, reticulocytosis, presence of spherocytes and polychromasia on the peripheral blood smear and a positive direct antiglobulin test (DAT). Increased indirect serum bilirubin, urinary urobilinogen and serum lactate dehydrogenase (LDH),
An adsorption procedure can be performed to remove the autoantibodies by adding target antigens (RBCs) and allowing the antibody to bind to the antigen. Autologous red blood cells can be used for adsorption in patients who have not been transfused recently (in the last three months). For patients who have been transfused recently, an allogeneic adsorption procedure can be performed. The alloadsorption requires donor red blood cells of three different phenotypes be used to remove the

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